Characterization of an understudied kinase, NEK5, in acquisition of a mesenchymaland migratory cell phenotype

一种正在研究的激酶 NEK5 在获取间充质细胞和迁移细胞表型中的表征

• 批准号: 10047560
• 负责人: Matthew E. Burow
• 金额: $ 16.68万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-08 至 2022-09-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10047560
• 关键词: Basic Science; Binding; Biological; Biological Assay; Biological Models; Biological Process; Biology; Breast Cancer Cell; Breast Cancer Model; Breast Cancer cell line; Cancer Biology; Cells; Cellular biology; Cessation of life; Chemicals; Clinic; Clinical; Communities; Data; Development; Disease; Disseminated Malignant Neoplasm; Distal; Drug Regulations; Drug Targeting; Drug resistance; Epithelial; Epithelium; FDA approved; Family; Family member; Follow-Up Studies; Foundations; Future; Gene Expression; Genome; Goals; Grant; Health; Human; Lead; Libraries; Link; Malignant Neoplasms; Mesenchymal; Mitosis; Modeling; Morphology; Nature; Neoplasm Metastasis; Oncology; Output; Pathologic; Pathway interactions; Patients; Pharmaceutical Chemistry; Phenotype; Phosphotransferases; Play; Positioning Attribute; Primary Neoplasm; Process; Proteins; Publishing; Pyrimidine; Reagent; Regulation; Reporting; Research; Resources; Role; Signal Pathway; Signal Transduction; Site; Structure-Activity Relationship; System; Techniques; Testing; Therapeutic; Tissues; Translational Research; Tumor Biology; Ursidae Family; Validation; Work; Xenograft Model; analog; cancer cell; cancer clinical trial; cancer subtypes; cancer therapy; cell motility; cellular targeting; clinical candidate; drug development; drug discovery; effective therapy; epithelial to mesenchymal transition; experience; experimental study; genetic manipulation; genome resource; human disease; human model; in vivo; in vivo evaluation; inhibitor/antagonist; interest; kinase inhibitor; malignant breast neoplasm; member; migration; multidisciplinary; novel; novel anticancer drug; novel therapeutics; scaffold; small molecule; small molecule inhibitor; success; targeted treatment; tool; triple-negative invasive breast carcinoma; tumor progression; tumorigenesis

Project Summary /Abstract Metastasis, or the spread of cancer cells from a primary tumor to distal tissue sites, is responsible for 90% of cancer-related deaths. Metastasis is a step-by-step process, with the major steps being initiation, invasion, migration, seeding, and colonization. Certain kinase families have been extensively studied as regulators of cell motility/invasion and epithelial-mesenchymal transition (EMT). Our preliminary data revealed members of the NEver-in-mitosis-related Kinase family (NEK5, NEK1 and NEK9) involved in the progression to the EMT phenotype. Previous studies demonstrate NEK9 and NEK1 are upstream regulators of PLK expression, but NEK5 remains uncharacterized. The use of kinase inhibitors to dissect and validate targetable nodes within cancer signaling pathways has revolutionized oncology drug discovery. Among the most interesting understudied IDG kinases is NEK5, a kinase linked to the critical biological process of epithelial to mesenchymal transition (EMT). Our preliminary evidence demonstrates that NEK5 is involved in pathways of significant pathological importance in difficult to treat breast cancer subtypes. There is an immediate need for potent, selective, and cell active NEK5 inhibitors that can be used to define the roles of NEK5 in EMT and evaluate the therapeutic potential of NEK5 inhibitors in relevant models of human disease. Here we propose a medicinal chemistry approach to optimize a promising inhibitor scaffold that we have identified and that has been declared an IDG tool molecule. In Aim 1 we will synthesize new analogues of the IDG tool in order to optimize cellular activity and selectivity. We will optimize cellular potency using an IDG resource assay: the NEK5 nanoBRET in cell target engagement assay. We will optimize selectivity as judged by broad kinome profile of our best molecules. The deliverable from Aim 1 will be a potent, cell active, narrow spectrum NEK5 inhibitor. These results will pave the way for further work to optimize this NEK5 scaffold into compounds that can be tested in vivo and in the clinic. In Aim 2 we will focus on delineating the biological role for NEK5 using triple negative breast cancer cell lines and patient derived xenograft model systems. To accomplish our goals, we have taken advantage of IDG published resources, and assembled a collaborative, multidisciplinary team with experience in kinase inhibitor optimization, cell biology, and tumor biology. Successful completion of this project will deliver a high quality NEK5 inhibitor, details on the roles NEK5 plays in the EMT, metastasis and tumorigenesis, and preliminary validation of NEK5 as a promising oncology drug discovery target for aggressive, hard to treat breast cancer subtypes. Results from experiments here may pave the way to new drugs for cancer treatment that target the understudied kinase NEK5. Free distribution of the NEK5 tool molecules will allow the community to determine other roles this IDG understudied kinase plays in signaling cascades in health and disease.

项目摘要/摘要