CAREER: Artificial Cell Membranes for Ultra-Pure, High Throughput Cellular Isolation

职业：用于超纯、高通量细胞分离的人造细胞膜

• 批准号: 1351531
• 负责人: Brad Berron
• 金额: $ 41.7万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-02-01 至 2020-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1351531&HistoricalAwards=false
• 关键词: CAREER; Artificial; Cell; Membranes; Ultra

CBET-1351531BerronCell-based therapies, where cells are transplanted to naturally restore biological function, represent an exciting future biomedical application. Critically, current techniques for high-purity cell sorting are time-intensive processes which lack the speed to satisfy the emerging widespread need for purified cells in research. The PI recently discovered a rapid cell isolation method in which batch size is virtually unlimited and nonspecific cells are undetected. The developed technology, Antigen Specific Lysis (ASL), selectively coats targeted cells in a temporary protective membrane, then destroys all uncoated cells to yield viable, purified cells. The proposed work will investigate fundamental mechanisms and relationships governing ASL in cellular isolation. This career development plan provides the foundation for a long term research program in the development ASL for the high speed isolation of rare biological products which yields a high-purity isolate from poorly-expressed markers. This research explores fundamental questions of cellular protection, and applies these findings to a cellular isolation technique which has the potential to reduce the presence of non-targeted cells to undetectable levels while sorting at exceptionally high rates. Bioseparations will play a critical role in the future of the US economy, yet many postsecondary institutions lack sufficient staffing and expertise to offer these courses. A distance learning Bioseparations course will be developed to reach geographically disparate institutions, enabling a robust offering of Bioseparations materials to students several hundreds of miles away. This course will serve as a platform to probe the limits of collaborative learning techniques in distance learning environments. A significant limitation to Bioseparations education is a lack of online learning tools. ASL is built upon a foundation of diverse Bioseparations principles (e.g. lysis, filtration, sedimentation, adsorption), and this career development plan uses ASL as the centerpiece to online Bioseparations screencasts - short educational videos. These videos are popular among students, yet are currently limited in Chemical Engineering to core courses. The developed screencasts will be disseminated in collaboration with the NSF-funded, LearnChemE.com site, where the existing screencasts have had 1.6 million views in the last 12 months. Further, the screencasts will be critiqued and edited by the LearnChemE.com team, ensuring the highest quality educational products.

CBET-1351531 Berron基于细胞的疗法，其中细胞被移植以自然恢复生物功能，代表了令人兴奋的未来生物医学应用。关键的是，目前的高纯度细胞分选技术是时间密集型过程，缺乏速度来满足研究中对纯化细胞的广泛需求。PI最近发现了一种快速细胞分离方法，其中批量大小几乎是无限的，并且未检测到非特异性细胞。开发的技术，抗原特异性裂解（ASL），选择性地在临时保护膜中包被靶细胞，然后破坏所有未包被的细胞以产生活的纯化细胞。拟议的工作将调查的基本机制和关系管理ASL细胞隔离。 该职业发展计划为ASL开发中的长期研究计划提供了基础，该计划用于稀有生物产品的高速分离，从而从低表达标记物中获得高纯度分离物。这项研究探讨了细胞保护的基本问题，并将这些发现应用于细胞分离技术，该技术有可能将非靶细胞的存在减少到无法检测的水平，同时以极高的速度进行分选。生物分离将在美国经济的未来中发挥关键作用，但许多高等教育机构缺乏足够的人员和专业知识来提供这些课程。将开发远程学习生物分离课程，以覆盖地理位置不同的机构，为数百英里外的学生提供强大的生物分离材料。本课程将作为一个平台，探讨远程学习环境中协作学习技术的局限性。 生物分离教育的一个重要限制是缺乏在线学习工具。ASL是建立在不同的生物分离原理（例如裂解，过滤，沉淀，吸附）的基础上，这个职业发展计划使用ASL作为在线生物分离屏幕播放的核心-简短的教育视频。这些视频在学生中很受欢迎，但目前仅限于化学工程核心课程。将与国家科学基金会资助的LearnChemE.com网站合作传播制作的屏幕录像，该网站现有的屏幕录像在过去12个月内有160万次浏览。此外，LearnChemE.com团队将对视频进行评论和编辑，以确保最高质量的教育产品。