EAGER: Assay Optimization for Highly Sensitive Detection of Human Cardiac Troponin I

该提案旨在开发和优化一种新的方法来检测与心血管疾病相关的血液标记物(肌钙蛋白I)。该生物标志物已被证明在心肌梗死的诊断中具有预测价值。因此，重要的是要在现场快速监测这种生物标志物--无论是在急救车上还是在急诊室里。这项建议通过简化用于分析肌钙蛋白生物标志物的传统夹心免疫分析/ELISA法，解决了快速检测肌钙蛋白的需要。这种简化的方法将只使用一种抗体/一步法来检测肌钙蛋白，并将涉及一种以受控方式定位于生物传感器表面的新型突变抗体。肌钙蛋白与这种荧光标记的抗体结合会导致荧光强度的变化。该项目的成功完成可能会导致生物传感器的未来发展，用于心肌梗死的快速、临床点诊断。

The proposal seeks to develop and optimize a novel approach for detection of a blood borne marker (Troponin I) associated with cardiovascular disease. This biomarker has been shown to have predictive value in diagnosis of myocardial infarctions. Therefore, it is important to monitor such biomarker rapidly and onsite - either in the first responder vehicle or in the emergency room. This proposal addresses the need for rapid detection of troponin by simplifying traditional sandwich immunoassay/ELISA used for analysis of this biomarker. This simplified assay will use just one antibody/one step for detection of troponin and will involve a novel mutant antibody oriented on the surfaces of the biosensor in a controlled manner. Troponin binding to this to this fluorescently labeled antibody will result in the change of fluorescence intensity. Successful completion of this project may lead to future development of biosensor for rapid, point of care diagnosis of myocardial infarctions.