EAGER: Assay Optimization for Highly Sensitive Detection of Human Cardiac Troponin I

该提案旨在开发和优化一种新的方法，用于检测与心血管疾病相关的血源性标志物（肌钙蛋白I）。 该生物标志物已被证明在心肌梗死的诊断中具有预测价值。因此，重要的是快速和现场监测这样的生物标志物-在第一响应者车辆中或在急诊室中。 该提案通过简化用于分析该生物标志物的传统夹心免疫测定/ELISA来解决快速检测肌钙蛋白的需求。 这种简化的测定将仅使用一种抗体/一个步骤来检测肌钙蛋白，并且将涉及以受控方式在生物传感器表面上定向的新型突变抗体。 肌钙蛋白与这种荧光标记抗体结合会导致荧光强度的变化。 该项目的成功完成可能会导致未来开发的生物传感器，用于快速，即时诊断心肌梗死。

The proposal seeks to develop and optimize a novel approach for detection of a blood borne marker (Troponin I) associated with cardiovascular disease. This biomarker has been shown to have predictive value in diagnosis of myocardial infarctions. Therefore, it is important to monitor such biomarker rapidly and onsite - either in the first responder vehicle or in the emergency room. This proposal addresses the need for rapid detection of troponin by simplifying traditional sandwich immunoassay/ELISA used for analysis of this biomarker. This simplified assay will use just one antibody/one step for detection of troponin and will involve a novel mutant antibody oriented on the surfaces of the biosensor in a controlled manner. Troponin binding to this to this fluorescently labeled antibody will result in the change of fluorescence intensity. Successful completion of this project may lead to future development of biosensor for rapid, point of care diagnosis of myocardial infarctions.