EAGER: New Approach for Biosensing in Cardiac Diagnosis

在美利坚合众国，心脏病是导致死亡的首要原因。每天有近2500名美国人死于心血管疾病，这些疾病夺走的生命比癌症、慢性呼吸系统疾病、事故和糖尿病加起来还要多。人心肌肌钙蛋白I(CTnI)具有较高的特异性，在心肌梗死的诊断中优于其他生物标志物。拟议研究的总体目标是基于荧光光谱技术，使用化学修饰的石英片和突变的单克隆鼠免疫球蛋白来检测cTnI。其具体目的是：(I)在空气-水界面上对免疫球蛋白突变体进行表面化学表征；(Ii)对适于化学连接突变体免疫球蛋白的固体底物进行表面修饰；(Iii)利用化学连接的突变体免疫球蛋白荧光团的荧光光谱技术检测cTnI。这一拟议项目的完成将为使用该生物标志物进行早期和快速诊断提供一条革命性的途径。它将开辟一条新的途径，直接检测血液样本中的cTnI，而不是通常使用表面等离子体共振光谱等光谱技术所使用的血浆和血清样本。该项目的变革性在于其使用化学结合的突变体免疫球蛋白进行检测的创新方法。

0944290LeblancIn the United States of America heart disease is the leading cause of death. Nearly 2500 Americans die of cardiovascular disease each day, which claims more lives than cancer, chronic respiratory diseases, accidents, and diabetes mellitus combined. Human cardiac troponin I (cTnI) is superior to other biomarkers in the diagnosis of myocardial infarction due to its specificity. The overall objective of the proposed research is the detection of cTnI based on the fluorescence spectroscopic technique using a chemically-modified quartz slide with a mutant monoclonal mouse IgG. The specific aims are: (i) Surface chemistry characterization of IgG mutants at the air-water interface; (ii) Surface modification of a solid substrate suitable for the chemical attachment of the mutant IgG; (iii) Detection of cTnI using fluorescence spectroscopic technique of the chemically-attached mutant IgG-fluorophore. Accomplishment of this proposed project will provide a revolutionary route for earlier and faster diagnosis using this biomarker. It will open a new avenue to directly detect the cTnI in a blood sample instead of the plasma and serum samples commonly used with spectroscopic techniques such as surface plasmon resonance spectroscopy. The transformative nature of this project lies in its innovative approach of detection using a chemically-attached mutant IgG.