Collaborative Research: Sense-and-Act Systems for Substance Release Modeling Drug Delivery Triggered by Immune-Sensing Based on Nanostructured Electrodes

提案：140328 /1403228 pi: Katz， Evgeny/Martinez-Lemus, LuisInstitution: Clarkson University/University of Missouri， columbia标题：合作研究：基于纳米结构电极的免疫传感触发的物质释放建模药物递送的感知和行为系统本研究项目将纳米结构生物传感系统与药物释放系统相结合。这项工作的变革性质在于整合电化学传感和电化学释放过程的新概念，有望在个性化药物管理方面发生革命性变化，特别是在紧急情况下以及在护理点和最终用户应用方面。具体来说，该项目旨在设计新系统，用于检测与糖尿病相关的关键疾病，检测后自动释放药物，以补偿医疗问题。这项研究最初是在模型系统中进行的，但最终将在动物研究中进行。该项目的技术描述本研究计划解决了开发用于传感非电化学活性生物分子的生物电子系统的关键挑战，例如蛋白质，以及氧化还原非活性低分子量物质。传感过程与驱动相结合，导致模拟药物释放的生物分子物质的信号触发释放，例如由酮体触发的胰岛素释放，酮体是酮症酸中毒的生物标志物。开发的生物电子系统集成了生物传感/免疫传感系统、生物燃料电池和信号触发物质释放系统的特征部件，从而代表了自主自供电操作的独特组件组合。具体来说，越来越复杂的模型系统被开发出来，可以通过利用附着的酶和免疫传感组件在纳米结构电极上产生负电位，以响应抗原。在连接电极系统的另一部分，产生的电位足以激活海藻酸盐层的溶解过程，在海藻酸盐层中，模拟药物的分子被捕获，从而触发药物释放。这种自供电和自激活的药物释放系统响应生物标志物传感，代表了“感知和行动”系统的新方法。

Proposal: 1403208/1403228PI: Katz, Evgeny/Martinez-Lemus, LuisInstitution: Clarkson University/University of Missouri, ColumbiaTitle: Collaborative Research: Sense-and-Act Systems for Substance Release Modeling Drug Delivery Triggered by Immune-Sensing Based on Nanostructured ElectrodesThis research program combines a nanostructured biosensing system with a drug-release system. The transformative nature of this work is in the novel concept of integrating the electrochemical sensing and electrochemical release processes, promising revolutionary changes in personalized drug administration, particularly in emergency situations and in point-of-care and end-user applications. Specifically, the program aims at designing new systems for detecting critical conditions associated with diabetes, with the detection followed by automated drug release to compensate for the medical problem. The research is initially carried out with model systems, but will culminate in animal study. Technical description of the projectThis research program addresses key challenges to developing bioelectronic systems for sensing non-electrochemically active biomolecules, e.g., proteins, as well as redox non-active low-molecular-weight substances. The sensing process is integrated with the actuation resulting in the signal-triggered release of biomolecular substances mimicking drug-release, for example, Insulin release triggered by ketone bodies that are biomarkers of ketoacidosis. The developed bioelectronic systems integrate parts characteristic of biosensing/immunesensing systems, biofuel cells and signal-triggered substance-releasing systems, thus representing a unique combination of components for autonomous self-powered operation. Specifically, model systems of increasing complexity are developed that can yield negative potential at a nanostructured electrode by utilizing attached enzymes and immunesensing assemblies, in response to antigens. The resulting potential suffices for activation, on another part of the connected electrode system, of the process of dissolution of an alginate layer in which the molecules mimicking drug are entrapped, triggering drug release. Such self-powered and self-activated drug-release systems operating in response to biomarker sensing represent novel approach to the "Sense-and-Act" systems.