Collaborative Research: Sense-and-Act Systems for Substance Release Modeling Drug Delivery Triggered by Immune-Sensing Based on Nanostructured Electrodes

合作研究：基于纳米结构电极的免疫传感触发的物质释放建模药物输送的感知与行动系统

• 批准号: 1403228
• 负责人: Luis Martinez-Lemus
• 金额: $ 4.96万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-15 至 2018-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1403228&HistoricalAwards=false
• 关键词: Collaborative; Research; Sense; Act; Systems

Proposal: 1403208/1403228PI: Katz, Evgeny/Martinez-Lemus, LuisInstitution: Clarkson University/University of Missouri, ColumbiaTitle: Collaborative Research: Sense-and-Act Systems for Substance Release Modeling Drug Delivery Triggered by Immune-Sensing Based on Nanostructured ElectrodesThis research program combines a nanostructured biosensing system with a drug-release system. The transformative nature of this work is in the novel concept of integrating the electrochemical sensing and electrochemical release processes, promising revolutionary changes in personalized drug administration, particularly in emergency situations and in point-of-care and end-user applications. Specifically, the program aims at designing new systems for detecting critical conditions associated with diabetes, with the detection followed by automated drug release to compensate for the medical problem. The research is initially carried out with model systems, but will culminate in animal study. Technical description of the projectThis research program address key challenges to developing bioelectronic systems for sensing non-electrochemically active biomolecules, e.g., proteins, as well as redox non-active low-molecular-weight substances. The sensing process is integrated with the actuation resulting in the signal-triggered release of biomolecular substances mimicking drug-release, for example, Insulin release triggered by ketone bodies that are biomarkers of ketoacidosis. The developed bioelectronic systems integrate parts characteristic of biosensing/immunesensing systems, biofuel cells and signal-triggered substance-releasing systems, thus representing a unique combination of components for autonomous self-powered operation. Specifically, model systems of increasing complexity are developed that can yield negative potential at a nanostructured electrode by utilizing attached enzymes and immunesensing assemblies, in response to antigens. The resulting potential suffices for activation, on another part of the connected electrode system, of the process of dissolution of an alginate layer in which the molecules mimicking drug are entrapped, triggering drug release. Such self-powered and self-activated drug-release systems operating in response to biomarker sensing represent novel approach to the ?Sense-and-Act? systems.

提案：1403208/1403228PI：Katz，Evgeny/Martinez-Lemus，路易斯安那研究所：克拉克森大学/密苏里大学，哥伦比亚省标题：合作研究：物质释放的感官与行为系统建模基于纳米结构电极的免疫传感触发的药物输送这项研究计划将纳米结构生物传感系统与药物释放系统相结合。这项工作的变革性本质在于集成了电化学传感和电化学释放过程的新概念，有望在个性化给药方面带来革命性的变化，特别是在紧急情况下以及在护理点和最终用户应用中。具体地说，该计划旨在设计新的系统来检测与糖尿病相关的危重情况，检测之后是自动药物释放，以弥补医疗问题。这项研究最初是通过模型系统进行的，但将在动物实验中达到顶峰。本研究计划致力于开发生物电子系统的关键挑战，用于检测非电化学活性生物分子，例如蛋白质以及氧化还原非活性低分子物质。传感过程与致动过程相结合，导致模拟药物释放的生物分子物质的信号触发释放，例如，由酮体触发的胰岛素释放，酮体是酮酸中毒的生物标志物。开发的生物电子系统集成了生物传感/免疫传感系统、生物燃料电池和信号触发物质释放系统的部分特性，从而代表了自主自给动力操作的独特组件组合。具体地说，开发了越来越复杂的模型系统，通过利用附着的酶和免疫传感组件来响应抗原，在纳米结构的电极上产生负电位。由此产生的电位足以在连接的电极系统的另一部分上激活海藻酸层的溶解过程，模拟药物的分子被包裹在海藻酸层中，引发药物释放。这种响应生物标记物传感的自给能和自激活药物释放系统代表了感知和行动？的新方法。系统。