Collision Cross Section Measurements Using Fourier Transform Ion Cyclotron Resonance Techniques

使用傅里叶变换离子回旋共振技术进行碰撞截面测量

• 批准号: 1412289
• 负责人: David Dearden
• 金额: $ 45.02万
• 依托单位: Brigham Young University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2018-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1412289&HistoricalAwards=false
• 关键词: Collision; Cross; Section; Measurements; Using

Professor David V. Dearden of Brigham Young University is supported by the Chemical Measurement and Imaging (CMI) Program in the Division of Chemistry and the Computational and Data-Enabled Science and Engineering Program to develop a technique to detect and characterize the shape of large molecules, commonly found in living systems and molecular-size devices. Such information is currently only available using highly specialized and expensive instruments. The new approach will require only minimal, inexpensive modifications of commercially-available instruments, thus making such measurements accessible to a wide array of scientists. The availability of this technique on affordable mass spectrometers is allowing the characterization of supramolecular assemblies and the study of peptide folding and protein conformational changes in fields such as supramolecular chemistry and biomolecular science. The results are expected to facilitate biomedical research and the design and manufacture of molecular-size devices, such as molecular switches and memory storage architectures.The method Dearden is developing is known by the acronym CRAFTI, cross sectional areas by Fourier Transform ion cyclotron resonance. It is used to characterize the shapes of supramolecular systems and large biomolecules through measurement of their collision cross sections in the gas phase. These measurements are accomplished by monitoring the signal due to the collisions of the ions with the molecules of a neutral background gas in a Fourier transform ion cyclotron resonance mass spectrometer (FTICR/MS). User-friendly software for CRAFTI data analysis is also being developed as part of this project, facilitating the transfer of the CRAFTI technology to the broader user community of FTICR and, potentially, to Orbitrap mass spectrometers. In the course of this research, graduate students are being trained in state-of-the-art mass spectrometric and analytical techniques. Dearden is recruiting talented undergraduate students and involving them in the research in preparation for future graduate studies. Results from this work are included in annual presentations to the general public as part of National Chemistry Week.

Brigham Young University的David V. Dearden教授得到了化学测量和成像（CMI）在化学部以及计算和数据支持的科学和工程计划中的支持，以开发一种技术，以检测和表征大型分子的形状，通常在生命系统和分子大小的启动中发现。 此类信息目前仅使用高度专业且昂贵的仪器可用。新方法只需要对商业上可用的工具进行最小的，廉价的修改，从而使各种各样的科学家可以访问此类测量。 该技术在负担得起的质谱仪上的可用性允许对超分子组件的表征以及对诸如超分子化学和生物分子科学等领域的肽折叠和蛋白质构象变化的研究。预计结果将促进生物医学研究以及分子大小设备的设计和制造，例如分子开关和存储存储架构。迪尔登的发展方法是由缩写的Crafti（由Fourier Transform transworce Cyclotron Resonance）所知道的。 它用于表征超分子系统的形状和通过测量其在气相中的碰撞横截面，以表征大分子的形状。这些测量方法是通过监测因离子与中性背景气体在傅立叶转化离子回旋共振质谱仪（FTICR/MS）中的碰撞引起的信号来完成的。作为该项目的一部分，还开发了用于CRAFTI数据分析的用户友好软件，从而促进了Crafti技术向FTICR的更广泛的用户社区的转移，并可能向Orbitrap质谱仪转移。在这项研究过程中，研究生正在接受最先进的质谱和分析技术的培训。迪尔登（Dearden）正在招募有才华的本科生，并让他们参与研究，以准备未来的研究生学习。作为国家化学周的一部分，这项工作的结果包括在向公众的年度演讲中。