Collision Cross Section Measurements Using Fourier Transform Ion Cyclotron Resonance Techniques
使用傅里叶变换离子回旋共振技术进行碰撞截面测量
基本信息
- 批准号:1904838
- 负责人:
- 金额:$ 49.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-07-01 至 2023-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
With support from the Chemical Measurement and Imaging program in the Chemistry Division, Professors David Dearden and Matthew Asplund of Brigham Young University and their students are devising a new approach to measuring the shapes of molecules, including the way that biological molecules like proteins are folded. While superb techniques exist for such measurements, those techniques require specialized instrumentation. This team is developing new techniques to extend these capabilities to an additional class of existing instrumentation with only minimal modification, making these measurements more widely available. Their approach may also offer enhanced performance for some applications. These measurements are important because just as a misshapen, misfolded parachute may not unfurl correctly, misfolded protein molecules (for example) do not function properly and are believed to be among the root causes of debilitating conditions like Alzheimer?s or Parkinson?s diseases. Sensitive detection of the shape is essential to understanding how molecules fit together and function, and what can go wrong at the molecular level. Students carrying out this work are trained in skills essential to the 21st century science that is the foundation of the U.S. economy.In this project, graduate and undergraduate students are developing techniques designed to measure the collision cross sections of gas phase ions, without requiring a dedicated ion mobility instrument. Two independent approaches to these measurements are taken. The first examines signal decay as ions are scattered out of a coherently-orbiting ensemble in a Fourier transform ion cyclotron resonance mass spectrometer (FTICR/MS). The decay rate (and the corresponding FTICR linewidth) depends on the collision cross section. The second approach measures the phase lag between the radiofrequency electric field used to excite the ions and the ion motion in the presence of a damping buffer gas. This approach overcomes mass-to-charge and energy limits that are inherent in the linewidth method. The utility of these techniques is demonstrated by examining problems in supramolecular chemistry and structural biochemistry. In the course of this work, graduate students are trained in advanced techniques for high performance mass spectrometry that are vital for the biotechnology industry and the emerging field of proteomics. Many of the talented undergraduate students engaged in this research subsequently pursue graduate studies. The instrumentation used in this study is also made available for undergraduate courses in general, analytical and physical chemistry at BYU, which is a significant supplier of B.S. chemistry and biochemistry students to U.S. graduate programs.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
在化学系化学测量和成像项目的支持下,杨百翰大学的大卫·迪尔登教授和马修·阿斯普伦德教授及其学生正在设计一种新的方法来测量分子的形状,包括蛋白质等生物分子的折叠方式。虽然存在用于这种测量的高超技术,但这些技术需要专门的仪器。 该团队正在开发新技术,以将这些功能扩展到另一类现有仪器,只需进行最小的修改,使这些测量更广泛地使用。 他们的方法还可以为某些应用程序提供增强的性能。 这些测量很重要,因为就像一个畸形的,错误折叠的降落伞可能无法正确展开一样,错误折叠的蛋白质分子(例如)不能正常发挥作用,并且被认为是阿尔茨海默氏症等衰弱性疾病的根本原因之一。s还是帕金森?的疾病。对形状的灵敏检测对于理解分子如何组合在一起和发挥作用以及在分子水平上可能出现什么问题至关重要。开展这项工作的学生将接受作为美国经济基础的21世纪科学所必需的技能培训。在该项目中,研究生和本科生将开发旨在测量气相离子碰撞截面的技术,而无需专用的离子迁移率仪器。 采取两种独立的方法来进行这些测量。第一个检查信号衰减的离子散射的相干轨道合奏在傅里叶变换离子回旋共振质谱仪(FTICR/MS)。衰减速率(和相应的FTICR线宽)取决于碰撞截面。第二种方法测量用于激发离子的射频电场与存在阻尼缓冲气体的离子运动之间的相位滞后。这种方法克服了线宽方法中固有的质荷比和能量限制。这些技术的效用是通过检查超分子化学和结构生物化学中的问题来证明的。在这项工作的过程中,研究生接受高性能质谱先进技术的培训,这些技术对生物技术产业和新兴的蛋白质组学领域至关重要。 许多有才华的本科生从事这项研究,随后追求研究生课程。 本研究中使用的仪器也可用于杨百翰大学的一般,分析和物理化学本科课程,杨百翰大学是BS的重要供应商。该奖项反映了NSF的法定使命,并被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。
项目成果
期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ion Mobility and Fourier Transform Ion Cyclotron Resonance Collision Cross Section Techniques Yield Long-Range and Hard-Sphere Results, Respectively
离子淌度和傅里叶变换离子回旋共振碰撞截面技术分别产生长程和硬球结果
- DOI:10.1021/jasms.2c00112
- 发表时间:2022
- 期刊:
- 影响因子:3.2
- 作者:Heravi, Tina;Arslanian, Andrew J.;Johnson, Spencer D.;Dearden, David V.
- 通讯作者:Dearden, David V.
Multi-CRAFTI: Relative Collision Cross Sections from Fourier Transform Ion Cyclotron Resonance Mass Spectrometric Line Width Measurements
Multi-CRAFTI:傅里叶变换离子回旋共振质谱线宽测量的相对碰撞截面
- DOI:10.1021/jasms.1c00297
- 发表时间:2022
- 期刊:
- 影响因子:3.2
- 作者:Pope, Brigham L.;Joaquin, Daniel;Hickey, Jacob T.;Mismash, Noah;Heravi, Tina;Shrestha, Jamir;Arslanian, Andrew J.;Anupriya;Mortensen, Daniel N.;Dearden, David V.
- 通讯作者:Dearden, David V.
Halide Size-Selective Binding by Cucurbit[5]uril–Alkali Cation Complexes in the Gas Phase
气相中葫芦[5]uril-碱金属阳离子配合物的卤化物尺寸选择性结合
- DOI:10.1021/acs.jpca.1c05060
- 发表时间:2021
- 期刊:
- 影响因子:0
- 作者:Heravi, Tina;Shen, Jiewen;Johnson, Spencer;Asplund, Matthew C.;Dearden, David V.
- 通讯作者:Dearden, David V.
Collision Cross-Section Measurements of Collision-Induced Dissociation Precursor and Product Ions in an FTICR-MS and an IM-MS: A Comparative Study
FTICR-MS 和 IM-MS 中碰撞诱导解离母离子和子离子的碰撞截面测量:比较研究
- DOI:10.1021/jasms.2c00089
- 发表时间:2022
- 期刊:
- 影响因子:3.2
- 作者:Arslanian, Andrew J.;Mismash, Noah;Dearden, David V.
- 通讯作者:Dearden, David V.
Prototypical Allosterism in a Simple Ditopic Ligand: Gas-Phase Topologies of Cucurbit[n]uril· n -Alkylammonium Complexes Controlled by Binding in the Second Site
简单双位配体中的典型变构现象:由第二位点结合控制的葫芦[n]脲·n-烷基铵配合物的气相拓扑
- DOI:10.1021/acs.jpca.2c01703
- 发表时间:2022
- 期刊:
- 影响因子:0
- 作者:Shrestha, Jamir;Porter, Savannah R.;Tinsley, Caleb;Arslanian, Andrew J.;Dearden, David V.
- 通讯作者:Dearden, David V.
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
David Dearden其他文献
David Dearden的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('David Dearden', 18)}}的其他基金
Collision Cross Section Measurements Using Fourier Transform Ion Cyclotron Resonance Techniques
使用傅里叶变换离子回旋共振技术进行碰撞截面测量
- 批准号:
1412289 - 财政年份:2014
- 资助金额:
$ 49.5万 - 项目类别:
Standard Grant
Gas Phase Supramolecular Characterization
气相超分子表征
- 批准号:
0957757 - 财政年份:2010
- 资助金额:
$ 49.5万 - 项目类别:
Standard Grant
Supramolecular Characterization via Mass Spectrometry
通过质谱法进行超分子表征
- 批准号:
0615964 - 财政年份:2006
- 资助金额:
$ 49.5万 - 项目类别:
Continuing Grant
NSF Young Investigator: Host-Guest Molecular Recognition in the Gas-Phase
NSF 青年研究员:气相中的主客体分子识别
- 批准号:
9496150 - 财政年份:1994
- 资助金额:
$ 49.5万 - 项目类别:
Continuing Grant
Molecular Recognition in the Gas Phase: Rates, Structures, and Energetics Without Solvents or Counterions
气相分子识别:无溶剂或抗衡离子的速率、结构和能量学
- 批准号:
9496141 - 财政年份:1994
- 资助金额:
$ 49.5万 - 项目类别:
Continuing Grant
NSF Young Investigator: Host-Guest Molecular Recognition in the Gas-Phase
NSF 青年研究员:气相中的主客体分子识别
- 批准号:
9357118 - 财政年份:1993
- 资助金额:
$ 49.5万 - 项目类别:
Continuing Grant
Molecular Recognition in the Gas Phase: Rates, Structures, and Energetics Without Solvents or Counterions
气相分子识别:无溶剂或抗衡离子的速率、结构和能量学
- 批准号:
9221224 - 财政年份:1993
- 资助金额:
$ 49.5万 - 项目类别:
Continuing Grant
相似国自然基金
胰岛素样生长信号介导的肺巨噬细胞和上皮细胞cross-tolk通过核自噬参与慢性气道炎症形成的机制研究
- 批准号:JCZRYB202500229
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
基于NLRP3炎性小体与自噬Cross-talk探讨心康冲剂干预心肌纤维化的机制研究
- 批准号:2025JJ80174
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
PKM2琥珀酰化修饰介导癌细胞与血小板间Cross-talk调控胆管癌侵袭转移的研究
- 批准号:JCZRYB202500379
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
基于破骨-成骨细胞Cross-Talk探讨内固定物存留对关节软骨退变的影响及补肾强骨治法的科学内涵
- 批准号:
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
三痹汤激活线粒体自噬影响免疫细胞Cross talk延缓椎间盘退变的机制研究
- 批准号:
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
多环芳烃(PAHs )介导的癌细胞和Th17细胞“cross-talk”所
致免疫抑制反应在胶质瘤增殖侵袭中的作用及毒理学机制研
究
- 批准号:
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
PGK1乙酰化修饰介导癌细胞与TAMs间Cross-talk调控胆囊癌EMT的研究
- 批准号:82373032
- 批准年份:2023
- 资助金额:49 万元
- 项目类别:面上项目
线粒体ClpP激动剂通过铁死亡-免疫调控cross-talk治疗急性髓细胞白血病的机制研究
- 批准号:82370171
- 批准年份:2023
- 资助金额:49 万元
- 项目类别:面上项目
生长激素受体通过脂肪酸介导的肝脏-脂肪Cross-talk影响脂质内稳态的研究
- 批准号:82370866
- 批准年份:2023
- 资助金额:49 万元
- 项目类别:面上项目
基于“cross-β折叠”结构形成理论解析面筋蛋白淀粉样纤维的形成机制
- 批准号:32372365
- 批准年份:2023
- 资助金额:50.00 万元
- 项目类别:面上项目
相似海外基金
Antiproton production cross section measurement of p+4He for dark matter research
用于暗物质研究的 p 4He 反质子产生截面测量
- 批准号:
23H01179 - 财政年份:2023
- 资助金额:
$ 49.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Cross Section Systematics and Neutrino Oscialltion Analysis in DUNE
DUNE 中的横截面系统学和中微子振荡分析
- 批准号:
2727053 - 财政年份:2022
- 资助金额:
$ 49.5万 - 项目类别:
Studentship
Compatibility of Sophistication of Wooden Fire-Resistant Structural Members and Cross-Section Control - Toward High-Rise Wooden Buildings
木质耐火结构构件的复杂性与截面控制的兼容性——面向高层木结构建筑
- 批准号:
21K20466 - 财政年份:2021
- 资助金额:
$ 49.5万 - 项目类别:
Grant-in-Aid for Research Activity Start-up
CAREER: A Keystone for Precision Neutrino Physics: Multi-Target Neutrino-Nucleus Cross Section Measurements with the Fermilab Short-Baseline Neutrino Program
职业:精密中微子物理的基石:利用费米实验室短基线中微子计划进行多目标中微子核横截面测量
- 批准号:
2047665 - 财政年份:2021
- 资助金额:
$ 49.5万 - 项目类别:
Continuing Grant
Visualization of vessel wall cross section by optimal coherence tomography in carotid endarterectomy
颈动脉内膜切除术中最佳相干断层扫描的血管壁横截面可视化
- 批准号:
21K08846 - 财政年份:2021
- 资助金额:
$ 49.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Improvement of prediction accuracy of advanced reactors: Precise and robust cross section adjustment method based on active subspace approach
先进反应堆预测精度的提高:基于主动子空间方法的精确鲁棒截面调整方法
- 批准号:
21K04940 - 财政年份:2021
- 资助金额:
$ 49.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study of collision cross section in lipid analysis using ion mobility-mass spectrometer
离子淌度质谱仪脂质分析中碰撞截面的研究
- 批准号:
21K06496 - 财政年份:2021
- 资助金额:
$ 49.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Measurement of the ttH production cross-section in the H to bb decay channel
H 至 bb 衰变通道中 ttH 生产横截面的测量
- 批准号:
2604920 - 财政年份:2021
- 资助金额:
$ 49.5万 - 项目类别:
Studentship
Measurement of antiproton production cross section near the threshold
阈值附近反质子产生截面的测量
- 批准号:
19K14747 - 财政年份:2021
- 资助金额:
$ 49.5万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Neutrino cross section measurements to solve the mystery of the matter-dominated universe
中微子截面测量可解开物质主导的宇宙之谜
- 批准号:
21K03588 - 财政年份:2021
- 资助金额:
$ 49.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)