MRI: Acquisition of a high resolution orbitrap liquid chromatograph tandem mass spectrometer for enhancing research and education

MRI：购买高分辨率轨道阱液相色谱串联质谱仪以加强研究和教育

• 批准号: 1428787
• 负责人: Stephen Bergmeier
• 金额: $ 39.84万
• 依托单位: Ohio University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2017-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1428787&HistoricalAwards=false
• 关键词: MRI; Acquisition; resolution; orbitrap; liquid

With this award from the Major Research Instrumentation Program (MRI) and support from the Chemistry Research Instrumentation Program (CRIF), Professor Stephen Bergmeier from Ohio University and colleagues Hao Chen, Michael Held II, Justin Holub and Marcia Kieliszewski will acquire a high resolution orbitrap liquid chromatograph tandem mass spectrometer (LC-MS/MS). In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. An instrument with a liquid chromatograph provides additional structural identification power by separating mixtures of compounds before they reach the mass spectrometer. In an orbitrap instrument there an outer section that acts as an electrode and a coaxial inner spindle-like electrode that traps ions in an orbital motion around the inner electrode. An image of the current is produced and converted to a mass spectrum using mathematical (Fourier transform) methods. This spectrometer will also be used in curricular activities involving students and researchers from the southeastern Ohio region and it will be used also by industrial partners.The proposal is aimed at enhancing research and education at all levels, especially in areas such as (a) the study of proteins involving elucidation of glycoprotein structures, probing protein redox chemistry, detection of miniature protein ligands and analyses of protein damage from oxidative stress; (b) characterizing synthesized drug candidates, natural products and their metabolites; and (c) establishing chemometric approaches for the identification of biomarkers and validation of chemometric models and algorithms.

在主要研究仪器计划（MRI）的奖励和化学研究仪器计划（CRIF）的支持下，来自俄亥俄州大学的Stephen Bergmeier教授及其同事Hao Chen，Michael Held II，Justin Holub和Marcia Kieliszewski将获得高分辨率轨道阱液相色谱串联质谱仪（LC-MS/MS）。一般来说，质谱（MS）是用于识别和表征嵌入复杂基质中的少量化学物质的关键分析方法之一。在典型的实验中，组分流入质谱仪，在那里它们被电离成母离子及其碎片离子，并测量它们的质量。这种高灵敏度的技术可以检测和确定复杂混合物中分子的结构。带有液相色谱仪的仪器通过在化合物到达质谱仪之前分离化合物的混合物来提供额外的结构鉴定能力。在轨道阱仪器中，有一个充当电极的外部部分和一个同轴的内部纺锤形电极，该电极捕获围绕内部电极轨道运动的离子。产生电流的图像，并使用数学（傅里叶变换）方法将其转换为质谱。该光谱仪还将用于涉及来自东南部俄亥俄州地区的学生和研究人员的课程活动中，也将被工业合作伙伴使用。该提案旨在加强各级研究和教育，特别是在以下领域：（a）蛋白质研究，涉及糖蛋白结构的阐明，探测蛋白质氧化还原化学，检测微型蛋白质配体和分析氧化应激引起的蛋白质损伤;（B）表征合成的候选药物、天然产物及其代谢物;和（c）建立用于鉴定生物标志物和验证化学计量学模型和算法的化学计量学方法。