SusChEM: Novel 1,2-Propanediol Biosynthesis from Renewable Feedstocks through Enzyme Discovery
SusChEM:通过酶发现从可再生原料生物合成新型 1,2-丙二醇
基本信息
- 批准号:1438332
- 负责人:
- 金额:$ 31.76万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-08-01 至 2018-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
SusChEM: Novel 1,2-Propanediol Biosynthesis from Renewable Feedstocks Through Enzyme DiscoveryAfter many years of practice, the chemical industry faces sustainability challenges that arise from both the potentially dwindling feedstocks and intermediates that are derived from fossil fuels, and from the increasingly undesirable environmental costs associated with their production. Biocatalytic syntheses, based on enzymatic conversion or microbial whole-cell synthesis, are increasingly explored as the alternate platform of chemical production. Key advantages of microbial synthesis over chemical syntheses include reaction selectivity, molecular diversity, and reduced environmental impact, and the utilization of renewable feedstocks, such as plant-based cellulosic material, instead of hydrocarbons. The successful implementation of microbial syntheses inherently addresses the sustainability challenges currently facing the chemical industry. Because natural pathways are often not suitable for large-scale chemical production, and do not lead to desired chemical products, efforts are needed into the discovery and the development of novel biosynthetic pathways for industrial chemicals. Professors Wei Niu and Jiantao Guo at the University of Nebraska-Lincoln propose to achieve sustainable production of an industrial bulk chemical, 1,2-propanediol (1,2-PDO), by developing a new biosynthetic route. 1,2 PDO may be integrated into the production of other chemicals, such as n-propanol and propylene. This study combines both the discovery and the engineering aspects of scientific research. UN-L Students participating in this work will gain perspectives on the important aspects and synergistic effects of integrating basic science and engineering. The multidisciplinary research activities will be used to support active recruitment of underrepresented undergraduate and graduate students to pursue studies and careers in STEM areas.The scientific goal of the proposed work is to establish the de novo biosynthesis of 1,2-propanediol, an industrial bulk chemical and a natural product, from renewable feedstocks through the reduction of a common fermentation product, lactic acid. Application of the two known 1,2-PDO biosynthetic routes is limited either by the scarce availability of the starting material or the involvement of cytotoxic biosynthetic intermediate. The PIs seek to overcome these limitations by establishing novel 1,2-PDO pathways using two parallel approaches. The first one is a discovery-driven approach that focuses on identifying the genetic and catalytic elements that function in the poorly understood lactic acid reduction pathway in Lactobacillus buchneri, which produces 1,2-PDO under anoxic growth conditions. The second one is a design and engineering-driven approach that focuses on the development of a novel artificial 1,2-PDO biosynthetic pathway, which entails the activation of lactic acid as lactoyl-CoA followed by two sequential reduction steps to form 1,2-PDO. This pathway would also enable the stereospecific biosynthesis of R- and S-1,2-PDO stereoisomers. Niu and Guo will apply protein engineering to improve the catalytic efficiency of the bottleneck enzyme, the CoA-dependent aldehyde dehydrogenase. To facilitate rational mutagenesis, efforts will be directed to obtain the crystal structure of the protein, of which the family is underrepresented in available protein structure database. In addition, the proposed work will establish a novel growth-coupled selection scheme to allow rapid sampling of large number of enzyme mutants. The selection scheme has the potential to be adapted for directed evolution of other enzymes that have similar cofactor requirement.
SusChEM:通过酶发现可再生原料合成新型1,2-丙二醇经过多年的实践,化学工业面临着可持续性挑战,这些挑战来自于化石燃料原料和中间体的潜在减少,以及与生产相关的日益不利的环境成本。生物催化合成,基于酶转化或微生物全细胞合成,越来越多地被探索作为化学生产的替代平台。与化学合成相比,微生物合成的主要优势包括反应选择性、分子多样性、减少对环境的影响,以及利用可再生原料,如植物基纤维素材料,而不是碳氢化合物。微生物合成的成功实施本质上解决了化学工业目前面临的可持续性挑战。由于自然途径往往不适合大规模化学生产,也不能产生理想的化学产品,因此需要努力发现和开发新的工业化学品生物合成途径。内布拉斯加大学林肯分校的牛伟教授和郭建涛教授提出,通过开发一种新的生物合成途径,实现工业散装化学品1,2-丙二醇(1,2- pdo)的可持续生产。1,2 PDO可以整合到其他化学品的生产中,如正丙醇和丙烯。这项研究结合了科学研究的发现和工程方面。参与这项工作的联合国- l学生将获得对基础科学与工程相结合的重要方面和协同效应的看法。多学科研究活动将用于支持积极招募代表性不足的本科生和研究生在STEM领域学习和就业。拟议工作的科学目标是通过减少一种常见的发酵产物乳酸,从可再生原料中建立1,2-丙二醇(一种工业散装化学品和天然产物)的从头生物合成。这两种已知的1,2- pdo生物合成途径的应用受到起始材料稀缺或细胞毒性生物合成中间体参与的限制。pi试图通过使用两种平行方法建立新的1,2- pdo途径来克服这些限制。第一个是一个发现驱动的方法,重点是鉴定在布氏乳杆菌乳酸还原途径中起作用的遗传和催化元件,该途径在缺氧生长条件下产生1,2- pdo。第二种是设计和工程驱动的方法,其重点是开发一种新的人工1,2- pdo生物合成途径,该途径需要将乳酸激活为乳酰辅酶a,然后进行两个连续还原步骤以形成1,2- pdo。该途径还可以实现R-和s -1,2- pdo立体异构体的立体特异性生物合成。牛和郭将应用蛋白质工程技术来提高瓶颈酶——辅酶a依赖性醛脱氢酶的催化效率。为了促进合理的诱变,将努力获得蛋白质的晶体结构,该家族在现有的蛋白质结构数据库中代表性不足。此外,所提出的工作将建立一种新的生长偶联选择方案,以允许快速采样大量的酶突变体。该选择方案有可能适用于具有类似辅因子需求的其他酶的定向进化。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Wei Niu其他文献
Multilayer Si shadow mask processing of wafer-scale MoS2 devices
晶圆级 MoS2 器件的多层 Si 荫罩加工
- DOI:
10.1088/2053-1583/ab6b6b - 发表时间:
2020 - 期刊:
- 影响因子:5.5
- 作者:
Haima Zhang;Xiaojiao Guo;Wei Niu;Hu Xu;Qijuan Wu;Fuyou Liao;Jing Chen;Hongwei Tang;Hanqi Liu;Zihan Xu;Zhengzong Sun;Zhijun Qiu;Yong Pu;Wenzhong Bao - 通讯作者:
Wenzhong Bao
Research on target detection method based on CNN
基于CNN的目标检测方法研究
- DOI:
10.1088/1742-6596/2252/1/012051 - 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
Wei Niu;Bo Gao;Wentao Zhan;Juan Cheng - 通讯作者:
Juan Cheng
MHC class I‐associated presentation of exogenous peptides is not only enhanced but also prolonged by linking with a C‐terminal Lys‐Asp‐Glu‐Leu endoplasmic reticulum retrieval signal
通过与 C 末端 Lys-Asp-Glu-Leu 内质网检索信号连接,MHC I 类相关的外源肽呈递不仅得到增强,而且得到延长
- DOI:
- 发表时间:
2004 - 期刊:
- 影响因子:5.4
- 作者:
Li Wang;Yuzhang Wu;An Chen;Jingbo Zhang;Zhao Yang;Wei Niu;Miao Geng;B. Ni;Wei Zhou;L. Zou;M. Jiang - 通讯作者:
M. Jiang
Approximate Analytical Solution to the Temperature Field in Annular Thermoelectric Generator Made of Temperature- Dependent Material
- DOI:
https://doi.org/10.1109/TED.2021.3122951 - 发表时间:
2021 - 期刊:
- 影响因子:
- 作者:
Wei Niu;Xiaoshan Cao;Yifeng Hu;Fangfang Wang;Junping Shi - 通讯作者:
Junping Shi
Probing the atomic-scale ferromagnetism in van der Waals magnet CrSiTe3
- DOI:
doi: 10.1063/5.0069885 - 发表时间:
2021 - 期刊:
- 影响因子:
- 作者:
Wei Niu;Xiaoqian Zhang;Wei Wang;Jiabao Sun;Yongbing Xu;Liang He;Wenqing Liu;Yong Pu - 通讯作者:
Yong Pu
Wei Niu的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Wei Niu', 18)}}的其他基金
Collaborative Research: OAC Core: CropDL - Scheduling and Checkpoint/Restart Support for Deep Learning Applications on HPC Clusters
合作研究:OAC 核心:CropDL - HPC 集群上深度学习应用的调度和检查点/重启支持
- 批准号:
2403090 - 财政年份:2024
- 资助金额:
$ 31.76万 - 项目类别:
Standard Grant
Engineering Carboxylic Acid Reductase for the Biosyntheses of Industrial Chemicals
用于工业化学品生物合成的工程羧酸还原酶
- 批准号:
1805528 - 财政年份:2018
- 资助金额:
$ 31.76万 - 项目类别:
Standard Grant
相似国自然基金
Novel-miR-1134调控LHCGR的表达介导拟
穴青蟹卵巢发育的机制研究
- 批准号:
- 批准年份:2025
- 资助金额:10.0 万元
- 项目类别:省市级项目
novel-miR75靶向OPR2,CA2和STK基因调控人参真菌胁迫响应的分子机制研究
- 批准号:82304677
- 批准年份:2023
- 资助金额:30.00 万元
- 项目类别:青年科学基金项目
海南广藿香Novel17-GSO1响应p-HBA调控连作障碍的分子机制
- 批准号:82304658
- 批准年份:2023
- 资助金额:30 万元
- 项目类别:青年科学基金项目
白术多糖通过novel-mir2双靶向TRADD/MLKL缓解免疫抑制雏鹅的胸腺程序性坏死
- 批准号:
- 批准年份:2021
- 资助金额:30 万元
- 项目类别:青年科学基金项目
novel_circ_001042/miR-298-5p/Capn1轴调节线粒体能量代谢在先天性肛门直肠畸形发生中的作用机制研究
- 批准号:
- 批准年份:2021
- 资助金额:55 万元
- 项目类别:面上项目
novel-miR-59靶向HMGAs介导儿童早衰症细胞衰老的作用及机制研究
- 批准号:
- 批准年份:2021
- 资助金额:58 万元
- 项目类别:面上项目
novel_circ_008138/rno-miR-374-3p/SFRP4调控Wnt信号通路参与先天性肛门直肠畸形发生的分子机制研究
- 批准号:82070530
- 批准年份:2020
- 资助金额:55 万元
- 项目类别:面上项目
miRNA-novel-272通过靶向半乳糖凝集素3调控牙鲆肠道上皮细胞炎症反应的机制研究
- 批准号:32002421
- 批准年份:2020
- 资助金额:24.0 万元
- 项目类别:青年科学基金项目
m6A修饰介导的lncRNA WEE2-AS1转录后novel-pri-miRNA剪切机制在胶质瘤恶性进展中的作用研究
- 批准号:
- 批准年份:2020
- 资助金额:55 万元
- 项目类别:面上项目
miRNA/novel_167靶向抑制Dmrt1的表达在红鳍东方鲀性别分化过程中的功能研究
- 批准号:31902347
- 批准年份:2019
- 资助金额:25.0 万元
- 项目类别:青年科学基金项目
相似海外基金
Understanding The Political Representation of Men: A Novel Approach to Making Politics More Inclusive
了解男性的政治代表性:使政治更具包容性的新方法
- 批准号:
EP/Z000246/1 - 财政年份:2025
- 资助金额:
$ 31.76万 - 项目类别:
Research Grant
PFI-TT: A Novel Wireless Sensor for Continuous Monitoring of Patients with Chronic Diseases
PFI-TT:一种用于持续监测慢性病患者的新型无线传感器
- 批准号:
2345803 - 财政年份:2024
- 资助金额:
$ 31.76万 - 项目类别:
Continuing Grant
NSF Postdoctoral Fellowship in Biology: Investigating a Novel Circadian Time-Keeping Mechanism Revealed by Environmental Manipulation
美国国家科学基金会生物学博士后奖学金:研究环境操纵揭示的新型昼夜节律机制
- 批准号:
2305609 - 财政年份:2024
- 资助金额:
$ 31.76万 - 项目类别:
Fellowship Award
STTR Phase I: Innovating Micro-Light Emitting Diode (LED) Manufacturing with Novel Quantum Dot Micro-Patterning Technology
STTR 第一阶段:利用新型量子点微图案化技术创新微发光二极管 (LED) 制造
- 批准号:
2335283 - 财政年份:2024
- 资助金额:
$ 31.76万 - 项目类别:
Standard Grant
A novel damage characterization technique based on adaptive deconvolution extraction algorithm of multivariate AE signals for accurate diagnosis of osteoarthritic knees
基于多变量 AE 信号自适应反卷积提取算法的新型损伤表征技术,用于准确诊断膝关节骨关节炎
- 批准号:
24K07389 - 财政年份:2024
- 资助金额:
$ 31.76万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Investigation of crosstalk between Fanconi Anemia pathway and ATM for novel therapeutic strategies of chemoresistant ALT-positive high-risk neuroblastoma
范可尼贫血通路与 ATM 之间的串扰研究,用于化疗耐药 ALT 阳性高危神经母细胞瘤的新治疗策略
- 批准号:
24K10442 - 财政年份:2024
- 资助金额:
$ 31.76万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Proof of alcoholic beverage consumption based on the quantitation of novel biomarkers
基于新型生物标志物定量的酒精饮料消费证明
- 批准号:
24K13564 - 财政年份:2024
- 资助金额:
$ 31.76万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mathematics to underpin and drive novel inertial microfluidic technologies
数学支撑和驱动新型惯性微流体技术
- 批准号:
DP240101089 - 财政年份:2024
- 资助金额:
$ 31.76万 - 项目类别:
Discovery Projects
A Novel Surrogate Framework for evaluating THM Properties of Bentonite
评估膨润土 THM 性能的新型替代框架
- 批准号:
DP240102053 - 财政年份:2024
- 资助金额:
$ 31.76万 - 项目类别:
Discovery Projects
EFR3: Novel gatekeeper of cell proliferation
EFR3:细胞增殖的新型看门人
- 批准号:
DP240102729 - 财政年份:2024
- 资助金额:
$ 31.76万 - 项目类别:
Discovery Projects