Maternal control of Pisum sativum embryo development: a role for X8CD1 and X8CD2 genes encoding small cystein-rich proteins

豌豆胚胎发育的母体控制：编码富含半胱氨酸的小蛋白的 X8CD1 和 X8CD2 基因的作用

• 批准号: 218061337
• 负责人: Dr. Ruslana Radchuk
• 金额: --
• 依托单位: Abteilung Molekulare Genetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/218061337?language=en
• 关键词: Maternal; control; Pisum; sativum; embryo

The dormant embryo in mature seeds of legumes and other species is dependent on the dry seed coat for structural protection and passive environmental sensing. During seed development, however, the maternal seed tissues play a much more vital and dynamic role. The legume embryo acquires oxygen, water, numerous phytohormones, and C and N assimilates through the seed coat. Although the particulars of this role have not been verified unequivocally, growing evidence demonstrates that seed coat nourishes and, to some extent, controls the development of the embryo. This project is designed to explore the role in embryo nutrition of specific novel genes expressed in the seed coat of pea and coding for small cystein-rich proteins (CRPs). Evidences from monocots suggest that CRPs are involved in the degradation of specific cell types, a process probably providing nutrients to the growing embryo. Transcript profiling and real-time (RT) qPCR will be used to characterize candidate genes. In order to elucidate their functions, transgenic loss-of-function pea plants will be generated and subjected to a comprehensive phenotypic and molecular analysis. The achieved results will provide new information regarding maternal control of dicot embryo development and its impact on seed development as a whole.

豆科植物和其他物种成熟种子中的休眠胚依赖于干燥的种皮进行结构保护和被动环境感知。然而，在种子发育过程中，母体种子组织起着更为重要和动态的作用。豆科植物的胚通过种皮获得氧气、水、大量的植物激素以及C和N。虽然这一作用的细节尚未得到明确证实，但越来越多的证据表明，种皮覆盖并在一定程度上控制着胚胎的发育。 本项目旨在探索豌豆种皮中表达的编码富含半胱氨酸的小蛋白（CRPs）的特定新基因在胚胎营养中的作用。来自单子叶植物的证据表明，CRPs参与了特定细胞类型的降解，这一过程可能为生长中的胚胎提供营养。转录谱分析和实时（RT）qPCR将用于表征候选基因。为了阐明它们的功能，将产生功能丧失的转基因豌豆植物并对其进行全面的表型和分子分析。所取得的成果将提供有关双子叶植物胚发育的母体控制及其对种子发育的整体影响的新信息。