CAREER: Biosensor Development for Probing Nanoscale Topology in Neurotransmission

职业:用于探测神经传递中纳米级拓扑的生物传感器开发

基本信息

  • 批准号:
    1452057
  • 负责人:
  • 金额:
    $ 50万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2015
  • 资助国家:
    美国
  • 起止时间:
    2015-01-15 至 2020-12-31
  • 项目状态:
    已结题

项目摘要

1452057 - KnowlesSensors that measure biological molecules are essential for medical diagnostics and drug discovery. These sensors often rely on the measurement of membrane proteins, which are targets for drug discovery. On the other hand, biomimetic systems provide simple platforms for incorporating membrane proteins into biosensing applications. In these applications, membrane proteins are able to sense their local environment, such as the chemical composition and membrane shape, and these factors affect protein function. Until now little attention has been given to the nanoscale structure of these sensors and how nanometer-sized features affect proteins. The main focus of this research is to design biosensors that can be used to identify how cells and biomolecules are affected by nanostructured materials. Specifically, proteins involved with the transmission of neuronal signals and the secretion of hormones will be characterized. The integration of research with education will take place through outreach to 8th-12th grade students in a summer engineering camp, training of local educators during a summer research program, and the design of novel courses at the interface of biochemistry and engineering.The engineering of materials that interface with biological systems increasingly requires an understanding of cellular and molecular responses to nanoscale topology. The focus of this CAREER Award is to design two biosensors that will be used to probe the relationship between nanostructured materials and protein function. The first sensor will mimic the intracellular plasma membrane with tunable regions of membrane curvature and chemical composition. The second will provide a template to introduce membrane curvature into live cells, where the molecular response to curvature can be assessed. Both sensors will be used to characterize the relationship between membrane shape and neurotransmission, a biological process that gives rise to extreme changes in membrane topology. Neurotransmission relies on the proper recruitment and function of SNARE proteins to facilitate the fusion of the tethered vesicle membrane with the plasma membrane. SNARE-mediated membrane fusion is essential for membrane repair, growth cone formation, axon extension, and synapse formation. Our work aspires to contribute to the field of neuroregeneration by identifying nanoscale features that drive membrane fusion. By using super-resolution fluorescence microscopy techniques, single particle tracking, and combined confocal fluorescence-atomic force microscopy, principles that govern protein sorting on nanostructured membranes will be identified. These principles will be useful in the future design of biosensors and materials that interface with cells. The integration of this research with education will take place through outreach to 8th-12th grade students in a summer engineering camp, training of local educators during a summer research program, and the design of novel courses at the interface of biochemistry and engineering.
1452057 -Knowles测量生物分子的传感器对于医学诊断和药物发现是必不可少的。这些传感器通常依赖于膜蛋白的测量,而膜蛋白是药物发现的目标。另一方面,仿生系统提供了简单的平台 为 结合 膜 蛋白 成 生物传感 应用. 在 在这些应用中,膜蛋白能够感知它们的局部环境,例如化学成分和膜形状,而这些因素影响蛋白质的功能。到目前为止,很少有人关注这些传感器的纳米结构以及纳米尺寸的特征如何影响蛋白质。这项研究的主要重点是设计可用于识别细胞和生物分子如何受到纳米结构材料影响的生物传感器。 具体而言,蛋白质参与神经元信号的传输和激素的分泌将被表征。研究与教育的整合将通过在暑期工程夏令营中对8 - 12年级学生的推广,在暑期研究计划中对当地教育工作者的培训以及生物化学和工程接口的新颖课程的设计来实现。与生物系统接口的材料工程越来越需要了解纳米级拓扑结构的细胞和分子反应。这个CAREER奖的重点是设计两个生物传感器,用于探测纳米结构材料和蛋白质功能之间的关系。第一个传感器将模拟细胞内质膜,具有膜曲率和化学组成的可调区域。第二个将提供一个模板,将膜曲率引入活细胞,在那里可以评估对曲率的分子反应。这两种传感器将用于表征膜形状和神经传递之间的关系,这是一个引起膜拓扑结构极端变化的生物过程。神经传递依赖于SNARE蛋白的适当募集和功能,以促进栓系囊泡膜与质膜的融合。SNARE介导的膜融合对于膜修复、生长锥形成、轴突延伸和突触形成至关重要。我们的工作渴望通过识别驱动膜融合的纳米级特征来为神经再生领域做出贡献。通过使用超分辨率荧光显微镜技术,单粒子跟踪,并结合共聚焦荧光原子力显微镜,管理纳米结构膜上的蛋白质分选的原则将被确定。 这些原则将在未来的生物传感器和与细胞接口的材料的设计中是有用的。这项研究与教育的整合将通过推广到8 - 12年级的学生在暑期工程营,在夏季研究计划期间培训当地教育工作者,并在生物化学和工程的界面设计新颖的课程。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Michelle Knowles其他文献

Effectiveness of a 'Green Card' Intervention for Patients Engaging in Deliberate Self-harm
“绿卡”干预对故意自残患者的有效性
  • DOI:
    10.1080/13811110301580
  • 发表时间:
    2003
  • 期刊:
  • 影响因子:
    2.8
  • 作者:
    M. Dudley;J. Beard;A. Clarke;Michelle Knowles;Richard Buss;V. Schnieden;S. Einfeld;M. Tobin;U. Dietrich
  • 通讯作者:
    U. Dietrich
Watching t-SNAREs And Their Interaction With Secretory Granules In Live Cells
  • DOI:
    10.1016/j.bpj.2008.12.3696
  • 发表时间:
    2009-02-01
  • 期刊:
  • 影响因子:
  • 作者:
    Wolfhard Almers;Michelle Knowles;Sebastian Barg;Lei Wan
  • 通讯作者:
    Lei Wan
Evidence-based practice for young people who self harm: can it be sustained and does it improve outcomes?
针对自残年轻人的循证实践:它能否持续并且是否能改善结果?
From efficacy to effectiveness: managing organisational change to improve health services for young people with deliberate self harm behaviour.
从功效到效果:管理组织变革,以改善对有故意自残行为的年轻人的健康服务。

Michelle Knowles的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Michelle Knowles', 18)}}的其他基金

Phospholipase D Regulation of Exosome Secretion
磷脂酶 D 对外泌体分泌的调节
  • 批准号:
    2325227
  • 财政年份:
    2023
  • 资助金额:
    $ 50万
  • 项目类别:
    Standard Grant
MCA - Application of quantitative imaging methods to identify molecular components of multi-vesicular body fusion sites
MCA - 应用定量成像方法识别多囊泡体融合位点的分子成分
  • 批准号:
    2122289
  • 财政年份:
    2021
  • 资助金额:
    $ 50万
  • 项目类别:
    Standard Grant
Progress Towards Understanding Neurotransmission: Temporal Mapping of Phospholipase D Activity in Exocytosis
了解神经传递的进展:胞吐作用中磷脂酶 D 活性的时间映射
  • 批准号:
    1807455
  • 财政年份:
    2018
  • 资助金额:
    $ 50万
  • 项目类别:
    Standard Grant
Collaborative Research: A Nanostructured Model of the Apoptotic Cell Surface
合作研究:凋亡细胞表面的纳米结构模型
  • 批准号:
    1033215
  • 财政年份:
    2010
  • 资助金额:
    $ 50万
  • 项目类别:
    Standard Grant

相似国自然基金

NAD+/NADH Biosensor “智能”调控好氧/厌氧耦合供给NADH产氢研究
  • 批准号:
    31970038
  • 批准年份:
    2019
  • 资助金额:
    58.0 万元
  • 项目类别:
    面上项目

相似海外基金

Enhancing Biosensor Surface Functionalization for new applications in Drug Development & Production
增强生物传感器表面功能化以实现药物开发的新应用
  • 批准号:
    10090247
  • 财政年份:
    2024
  • 资助金额:
    $ 50万
  • 项目类别:
    Collaborative R&D
Development of an apoptosis biosensor for monitoring of breast cancer
开发用于监测乳腺癌的细胞凋亡生物传感器
  • 批准号:
    10719415
  • 财政年份:
    2023
  • 资助金额:
    $ 50万
  • 项目类别:
Development of a Fully Wireless Biosensor Using Electrochemiluminescence Operated by Wireless Power Transmission
利用无线电力传输操作的电化学发光开发全无线生物传感器
  • 批准号:
    23K17853
  • 财政年份:
    2023
  • 资助金额:
    $ 50万
  • 项目类别:
    Grant-in-Aid for Challenging Research (Exploratory)
Development of A Novel Nanoparticle Biosensor for Rapid, Point-of-Care Sepsis Diagnosis and Risk Assessment
开发新型纳米颗粒生物传感器,用于快速护理点脓毒症诊断和风险评估
  • 批准号:
    10602155
  • 财政年份:
    2023
  • 资助金额:
    $ 50万
  • 项目类别:
Development of a minimally invasive optical biosensor to improve hyperphosphatemia management
开发微创光学生物传感器以改善高磷血症管理
  • 批准号:
    10742278
  • 财政年份:
    2023
  • 资助金额:
    $ 50万
  • 项目类别:
SBIR Phase I: Development of an Optical Biosensor to Facilitate Screening, Validation, and Development of Novel Drugs and their Biological Targets
SBIR 第一阶段:开发光学生物传感器以促进新药及其生物靶点的筛选、验证和开发
  • 批准号:
    2129469
  • 财政年份:
    2022
  • 资助金额:
    $ 50万
  • 项目类别:
    Standard Grant
Development of a low cost, label-free, and highly sensitive optical cavity-based biosensor for point-of-care diagnostics
开发用于即时诊断的低成本、无标记且高灵敏度的基于光学腔的生物传感器
  • 批准号:
    10439062
  • 财政年份:
    2022
  • 资助金额:
    $ 50万
  • 项目类别:
Development of a robotic directed evolution approach to whole cell biosensor production
开发用于全细胞生物传感器生产的机器人定向进化方法
  • 批准号:
    2742540
  • 财政年份:
    2022
  • 资助金额:
    $ 50万
  • 项目类别:
    Studentship
Development of a hydrogel microneedle based biosensor for diabetes management
开发用于糖尿病管理的基于水凝胶微针的生物传感器
  • 批准号:
    572998-2022
  • 财政年份:
    2022
  • 资助金额:
    $ 50万
  • 项目类别:
    University Undergraduate Student Research Awards
Portable Thermophotonic Biosensor Development for in-vivo Blood Glucose Monitoring
用于体内血糖监测的便携式热光子生物传感器的开发
  • 批准号:
    572588-2022
  • 财政年份:
    2022
  • 资助金额:
    $ 50万
  • 项目类别:
    University Undergraduate Student Research Awards
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了