IDBR: TYPE A: Quantitative Polarization and Phase Microscope for label-free imaging of live cell and tissue dynamics

IDBR：A 型：定量偏振和相位显微镜，用于活细胞和组织动力学的无标记成像

• 批准号: 1455630
• 负责人: Rongguang Liang
• 金额: $ 61.38万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1455630&HistoricalAwards=false
• 关键词: IDBR; TYPE; Quantitative; Polarization; Phase

An award is made to The University of Arizona to develop a Quantitative Polarization and Phase Microscope (QP2M). QP2M can measure the spectral dependent quantitative polarization properties and phase information of cell and tissue in real time; it is insensitive to motion and vibration because all information is acquired in a single shot. The microscope measures all four properties of light interacting with cell and tissue: intensity, wavelength, polarization, and phase. The ability to instantaneously measure live cells and follow motions and processes over time provides valuable information to the study of cellular dynamics, motility, and cell and tissue morphology. This project will bring multidisciplinary research and education in optical sciences and biology to both undergraduates and graduates students through the research in microfabrication, optical instrumentation, and bioimaging.The goal of this project is to integrate quantitative polarization and phase imaging techniques for label-free imaging of live cell dynamics. Novel color polarization sensor will be developed to detect right circular polarized light and three linearly polarized light at 0, 45, and 90 degree angles, at three wavelengths in a single shot. The sensor will be integrated with a microscope that can simultaneously capture 3 phase-shift images for quantitative phase measurement or 4 images with different polarization states for quantitative polarization measurement. The fast acquisition capacity of polarization and phase imaging will allow the coupling of spectral, phase, and polarization analysis and may reveal previously unattained vital information about the dynamics in living cells, tissues, and whole organisms, leading to new discoveries not possible before. The polarization and phase imaging techniques being developed for this project also enable new applications and experiments in cancer detection, diseases diagnosis and surgery.

授予亚利桑那大学的奖励，以开发定量极化和相显微镜（QP2M）。 QP2M可以实时测量依赖频谱依赖性的定量极化特性以及细胞和组织的相信息；它对运动和振动不敏感，因为所有信息均以单镜头获取。显微镜测量光与细胞和组织相互作用的所有四个特性：强度，波长，极化和相。 立即测量活细胞并遵循随着时间的运动和过程的能力为细胞动力学，运动能力以及细胞和组织形态的研究提供了有价值的信息。该项目将通过微型制动，光学仪器和生物成像的研究将光学科学和生物学的多学科研究和教育带给本科生和毕业生。该项目的目的是整合定量偏振和相位成像技术，以实现无效电池动力学的无标记成像。将开发新型的颜色偏振传感器，以检测右圆极化光和三个线性极化的光在0、45和90度角，单个镜头三个波长。传感器将与显微镜集成，该显微镜可以同时捕获3个相移图像以进行定量相测量或具有不同极化状态的4个图像以进行定量极化测量。极化和相成像的快速获取能力将允许光谱，相位和极化分析的耦合，并可能揭示先前未触及的有关生物细胞，组织和整个生物体动态的重要信息，从而导致以前无法发现新发现。该项目开发的极化和相成像技术还可以在癌症检测，疾病诊断和手术中进行新的应用和实验。