Molecular correlates of memory phases and development of molecular tools to modify brain physiology in the honeybee.

记忆阶段的分子相关性和改变蜜蜂大脑生理学的分子工具的开发。

• 批准号: 219171051
• 负责人: Dr. Gérard Leboulle
• 金额: --
• 依托单位: Bereich Neurobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/219171051?language=en
• 关键词: Molecular; correlates; memory; phases; development

In a previous study in the honeybee, Apis mellifera, we showed that the inhibition of the NR1 subunit of the NMDA glutamate receptor by RNA interference impairs early memory phases but leaves intact a late memory phase. This result is surprising because other studies show that the receptor is required during learning for the development of all memory phases. The inhibition induced by RNA interference was of moderate amplitude and it was limited in time and space. One goal of the project is to evaluate if these limitations explain the lack of effect on the late memory phase. However, other factors might be important for this memory phase. The target of rapamycin, the eukaryotic elongation factor 2 and voltage-gated calcium channels are possible candidates that will be considered in this project. There is evidence of an inhibitory neurotransmission mediated by glutamate chloride channels. We have shown by inducing RNA interference against the GluClα subunit that these channels of the mushroom body region are important for the retrieval of olfactory memory. Another goal of the project is to evaluate the role of antennal lobe channels in olfaction and in olfactory memory.

在以前的研究中，蜜蜂，蜜蜂，我们表明，抑制NMDA谷氨酸受体的NR1亚基的RNA干扰损害早期记忆阶段，但留下完整的晚期记忆阶段。这一结果令人惊讶，因为其他研究表明，在学习过程中，所有记忆阶段的发展都需要受体。RNA干扰抑制作用强度中等，且受时间和空间的限制。该项目的一个目标是评估这些限制是否可以解释对后期记忆阶段缺乏影响。然而，其他因素可能对这个记忆阶段很重要。雷帕霉素的靶点，真核细胞延伸因子2和电压门控钙通道是本项目可能考虑的候选者。有证据表明谷氨酸氯离子通道介导的抑制性神经传递。我们已经通过诱导RNA干扰GluClα亚基，证明蘑菇体区域的这些通道对于嗅觉记忆的恢复是重要的。该项目的另一个目标是评估触角叶通道在嗅觉和嗅觉记忆中的作用。

项目成果

Honey bee promoter sequences for targeted gene expression

用于靶向基因表达的蜜蜂启动子序列

• DOI: 10.1111/imb.12031
• 期刊: Insect Molecular Biology
• 影响因子: 2.6
• 作者: Schulte C;Leboulle G;Otte M;Grünewald B;Gehne N;Beye M.
• 通讯作者: Beye M.