DISSERTATION RESEARCH: Evolutionary Trends Within the Polemoniaceae (Ericales)

论文研究：杜鹃花科（Ericales）的进化趋势

• 批准号: 1501867
• 负责人: Kenneth Sytsma
• 金额: $ 1.71万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1501867&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Evolutionary; Trends; Within

The Polemoniaceae, the phlox family, is a textbook example of an adaptive radiation and has long been studied by biologists from diverse fields under the assumption that pollinators have played a key role in the formation of its species. However, this, hypothesis remains untested. This research explores evolution in the phlox family, a group of plants with most species found in the United States to test this this hypotheses from ecological and genetic perspectives. This research will benefit science and society by developing identification keys which will allow the public, especially members of native plant organizations, to identify and therefore have a deeper appreciation for the biological diversity of phlox. These data will also allow conservationists to make informed, cost-effective decisions as they seek to preserve endangered and threatened species at both the State and Federal level, especially in California, Nevada, Minnesota, and Wisconsin.Polemonium has been the subject of multiple molecular phylogenetic studies yet the relationships among its species remain undetermined. Species boundaries between species are also unclear, perhaps as a result of reticulate evolution. This project will generate a near species-level phylogeny of the Polemoniaceae and will also use morphometric techniques to generate trait data. This study will be the first to sample all species and utilize multiple nuclear loci to determine species relationships. These data will test the hypothesis that floral form has been selected for by pollinators and the morphometric data will determine the evolution of leaf form. Collection data from natural history museums will be used to investigate the biogeographic history of Polemonium. The use of comparative phylogenetic methods with these data sets provides a unique opportunity to reconstruct the ancestral character states at various time periods during phlox evolution. Polemonium occidentale ssp. lacustre is endangered in both states in which it occurs, but is not yet Federally listed as endangered. Next-generation sequencing approaches will be used to determine its taxonomic status and population structure to inform conservation of this rare taxon.

福禄考科植物是适应性辐射的典型代表，长期以来，来自不同领域的生物学家一直认为传粉者在其物种的形成过程中发挥了关键作用，因此对其进行了研究。然而，这一假设仍未得到检验。这项研究探索了福禄克族的进化，福禄克族是在美国发现的一组物种最多的植物，以从生态学和遗传学的角度验证这一假说。这项研究将使科学和社会受益，因为它将使公众，特别是本土植物组织的成员能够识别并因此更深入地了解福禄克斯的生物多样性。这些数据还将使自然资源保护者在寻求在州和联邦层面上保护濒危和受威胁物种时，做出明智、经济有效的决定，特别是在加利福尼亚州、内华达州、明尼苏达州和威斯康星州。Polemonium一直是多个分子系统学研究的主题，但其物种之间的关系仍未确定。物种之间的物种边界也不清楚，可能是网状进化的结果。该项目将生成一个接近物种水平的Polemoniaceae的系统发育图，并将使用形态测量技术来生成特征数据。这项研究将首次对所有物种进行采样，并利用多个核基因座来确定物种之间的关系。这些数据将检验传粉者已经选择了花形的假设，而形态测量数据将决定叶形的进化。来自自然历史博物馆的收集数据将用于调查Polemonium的生物地理史。对这些数据集的比较系统发育方法的使用提供了一个独特的机会来重建在福洛斯进化过程中不同时期的祖先特征状态。西花菜Polemonum ocicientale SSP.湖泊在发生的两个州都是濒危物种，但尚未被联邦列为濒危物种。下一代测序方法将用于确定其分类地位和种群结构，为保护这一稀有分类群提供信息。