DISSERTATION RESEARCH: Evolutionary Trends Within the Polemoniaceae (Ericales)

论文研究：杜鹃花科（Ericales）的进化趋势

• 批准号: 1501867
• 负责人: Kenneth Sytsma
• 金额: $ 1.71万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1501867&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Evolutionary; Trends; Within

The Polemoniaceae, the phlox family, is a textbook example of an adaptive radiation and has long been studied by biologists from diverse fields under the assumption that pollinators have played a key role in the formation of its species. However, this, hypothesis remains untested. This research explores evolution in the phlox family, a group of plants with most species found in the United States to test this this hypotheses from ecological and genetic perspectives. This research will benefit science and society by developing identification keys which will allow the public, especially members of native plant organizations, to identify and therefore have a deeper appreciation for the biological diversity of phlox. These data will also allow conservationists to make informed, cost-effective decisions as they seek to preserve endangered and threatened species at both the State and Federal level, especially in California, Nevada, Minnesota, and Wisconsin.Polemonium has been the subject of multiple molecular phylogenetic studies yet the relationships among its species remain undetermined. Species boundaries between species are also unclear, perhaps as a result of reticulate evolution. This project will generate a near species-level phylogeny of the Polemoniaceae and will also use morphometric techniques to generate trait data. This study will be the first to sample all species and utilize multiple nuclear loci to determine species relationships. These data will test the hypothesis that floral form has been selected for by pollinators and the morphometric data will determine the evolution of leaf form. Collection data from natural history museums will be used to investigate the biogeographic history of Polemonium. The use of comparative phylogenetic methods with these data sets provides a unique opportunity to reconstruct the ancestral character states at various time periods during phlox evolution. Polemonium occidentale ssp. lacustre is endangered in both states in which it occurs, but is not yet Federally listed as endangered. Next-generation sequencing approaches will be used to determine its taxonomic status and population structure to inform conservation of this rare taxon.

Polemoniaceae，即福禄考家族，是适应性辐射的教科书例子，长期以来，来自不同领域的生物学家一直在研究，假设传粉媒介在其物种的形成中发挥了关键作用。然而，这一假设尚未得到检验。这项研究探索了福禄考家族的进化，福禄考是在美国发现的大多数种类的植物，从生态和遗传的角度来检验这个假设。 这项研究将通过开发识别密钥来造福科学和社会，使公众，特别是本土植物组织的成员，能够识别福禄考的生物多样性，从而对福禄考的生物多样性有更深入的了解。 这些数据还将使自然资源保护主义者能够在州和联邦层面，特别是在加利福尼亚州、内华达州、明尼苏达州和威斯康星州寻求保护濒临灭绝和受威胁物种时，做出明智的、具有成本效益的决策。钋一直是多项分子系统发育研究的主题，但其物种之间的关系仍未确定。物种之间的物种界限也不清楚，这可能是网状进化的结果。该项目将生成 Polemoniaceae 近物种水平的系统发育，并将使用形态测量技术生成性状数据。 这项研究将是第一个对所有物种进行采样并利用多个核基因座来确定物种关系的研究。这些数据将检验传粉媒介选择花形的假设，而形态测量数据将确定叶形的演变。 自然历史博物馆的收藏数据将用于调查 Polemonia 的生物地理历史。 使用这些数据集进行比较系统发育方法为重建福禄考进化过程中不同时期的祖先特征状态提供了独特的机会。 Polemium occidentale ssp. lacustre 在其发生的两个州都处于濒危状态，但尚未被联邦列为濒危物种。 下一代测序方法将用于确定其分类地位和种群结构，以便为这种稀有分类群的保护提供信息。