Development of a highly sensitive detection platform based on metallic nanoparticle hydrogel composites in a microfluidic device

微流控装置中基于金属纳米颗粒水凝胶复合材料的高灵敏度检测平台的开发

• 批准号: 576645-2022
• 负责人: Hwang, DaeKunDK
• 金额: $ 1.82万
• 依托单位: Ryerson University
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=747039
• 关键词: Development; highly; sensitive; detection; platform

Diagnostics are an essential part of the healthcare system. Early and accurate detection of disease indicators are vital for reducing transmission and successful treatments. Building upon Dr. Dae Kun Hwang's previous work, Dr. Hwang has invented a highly rapid and simultaneous multiplexed immunoassay platform based on a hydrogel membrane to address this challenge. This will detect and quantify various biomarkers concurrently in the same sample. Microfluidics also requires smaller samples which are cheaper and easier to automate. These advantages will significantly reduce testing and detection time. However, this hydrogel membrane microfluidic platform suffers from low sensitivity compared to highly ultra-sensitive detection devices, such as electrospray mass spectrometry. Via the proposed international collaboration with Dr. Younghum Kim's research team, Dr. Hwang will encapsulate metallic nanoparticles such as silver, synthesized by Dr. Kim's team, into the hydrogel to overcome the low sensitivity limitation. This nanoparticle encapsulation will significantly enhance the detection signals of target biomolecules. Dr. Kim's and Dr. Hwang's research teams will investigate the effects of the size, shape, and concentration of metallic nanoparticles on signal enhancement. Dr. Hwang's innovative and versatile biomedical tools will allow him to improve the quality of healthcare and wellbeing on a large scale.

诊断是医疗保健系统的重要组成部分。及早准确地检测疾病指标对于减少传播和成功治疗至关重要。在Dae Kun Hwang博士之前工作的基础上，Hwang博士发明了一种基于水凝胶膜的快速和同步多重免疫分析平台来应对这一挑战。这将同时检测和量化同一样本中的各种生物标志物。微流体还需要更小的样品，更便宜，更容易自动化。这些优势将显著减少测试和检测时间。然而，与电喷雾质谱仪等高灵敏度检测设备相比，这种水凝胶膜微流控平台的灵敏度较低。通过拟议的与Young ghum Kim博士研究团队的国际合作，Hwang博士将把Kim博士团队合成的银等金属纳米颗粒封装到水凝胶中，以克服低灵敏度的限制。这种纳米颗粒的包裹将显著增强目标生物分子的检测信号。Kim博士和Hwang博士的研究团队将研究金属纳米颗粒的大小、形状和浓度对信号增强的影响。黄博士的创新和多功能生物医学工具将使他能够大规模提高医疗保健和福祉的质量。