Collaborative Research: Injectable, Biocompatible, Programmed-Bioresorbable Nanosensor Array for In-Vivo Continuous Glucose Monitoring

合作研究：用于体内连续血糖监测的可注射、生物相容性、程序化生物可吸收纳米传感器阵列

• 批准号: 1506966
• 负责人: Hanchen Huang
• 金额: $ 9.7万
• 依托单位: Northeastern University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-15 至 2018-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1506966&HistoricalAwards=false
• 关键词: Collaborative; Research; Injectable; Biocompatible; Programmed

Diabetes is a metabolic disorder and major world health problem. As stated by the International Diabetes Federation, there are over 350 million diabetics worldwide causing over 3.2 million deaths annually. Consequently, glucose detection is of paramount importance in the diagnosis and treatment of diabetes. This project will illustrate how nanotechnology, biomaterials, and biosensing technologies can be meshed together for injectable, biocompatible, bioresorbable nanosensor array for continuous glucose monitoring. The work will advance in vitro and/or in vivo monitoring technology for continuous glucose monitoring in general and, if successful, will not only have an enormous impact on public health, but also provide a demonstration from basic science to applications. This project will impact education of the graduate, undergraduate and high school students by integrating advanced biosensing into their educational and laboratory training, and actively interact with the general public and industry companies through various outreach activities within and outside of the University of Connecticut and Northeastern University. Moreover, a new course of Bioanalytical Sensor will be offered to students. This multidisciplinary proposal aims to develop an injectable biocompatible and programmed-bioresorbable surface-enhanced Raman scattering sensor for long-term, subcutaneous, continuous glucose monitoring. A number of novel features crossing bio-, nano-, material- and sensing technologies will be seamlessly meshed into this novel glucose sensing system. A sandwich-like glucose sensing material, including programmed-bioresorbable glucose-responsive nanosensor array covered by biocompatible and biodegradable hydrogel with entrapped anti-inflammatory drug, will be fabricated. Then a sterilized indwelling needle will be used to deliver the as-prepared sensor subcutaneously for long-term, continuous glucose sensing. The sensing performance will be systematically investigated. The synergistic effects of (1) good biocompatibility and biodegradability of hydrogel, together with (2) good biocompatibility and stability of nanosensor array, (3) programmable bioresorbability of sensing materials after long-term use, (4) specific and reversible binding of glucose with sensing material and its unique and strong glucose-responsive optical signals distinguishable from those of skin tissue and hydrogel, (5) non-invasive monitoring through Raman scattering, (6) anti-inflammatory effect provided by anti-inflammatory drug entrapped in hydrogel, and (7) minimally invasive delivery of sensor through convenient skin piercing, will accomplish the goal of reliable long-term continuous glucose monitoring.

糖尿病是一种代谢性疾病，也是主要的世界性健康问题。正如国际糖尿病联合会所指出的那样，全世界有超过3.5亿糖尿病患者，每年导致超过320万人死亡。因此，血糖检测在糖尿病的诊断和治疗中至关重要。该项目将说明如何将纳米技术、生物材料和生物传感技术结合在一起，形成可注射、生物兼容、可生物吸收的纳米传感器阵列，用于连续监测血糖。这项工作将推动血糖连续监测的体外和/或体内监测技术的发展，如果成功，不仅将对公众健康产生巨大影响，而且将为从基础科学到应用提供示范。该项目将通过将先进的生物传感整合到他们的教育和实验室培训中，影响研究生、本科生和高中生的教育，并通过康涅狄格大学和东北大学内外的各种外联活动，积极与普通公众和行业公司互动。此外，还将开设一门新的生物分析传感器课程。这项多学科的建议旨在开发一种可注射、生物相容和可编程生物可吸收的表面增强拉曼散射传感器，用于长期、皮下、连续的血糖监测。许多跨越生物、纳米、材料和传感技术的新特征将无缝地融入这个新的葡萄糖传感系统。将制备一种三明治状的葡萄糖传感材料，包括可编程生物可吸收葡萄糖响应型纳米传感器阵列，表面覆盖有生物相容和可生物降解的水凝胶，并包裹抗炎药物。然后，使用消毒的留置针将所制备的传感器输送到皮下，进行长期、连续的血糖检测。系统地研究了该传感器的传感性能。(1)水凝胶良好的生物相容性和生物降解性，(2)纳米传感器阵列良好的生物相容性和稳定性，(3)传感材料长期使用后的可编程生物吸收，(4)葡萄糖与传感材料的特异性和可逆性结合，以及其独特而强烈的葡萄糖响应光学信号与皮肤组织和水凝胶的区别，(5)通过拉曼散射进行无创监测，(6)抗炎药在水凝胶中提供的抗炎作用，(7)通过方便的皮肤穿孔以微创的方式输送传感器，将达到可靠的长期连续血糖监测的目的。