UNS: Non-Optical Detection of Biomolecular Binding Events at Electrical Liquid Interfaces

UNS：电液界面生物分子结合事件的非光学检测

• 批准号: 1511185
• 负责人: Zachary Gagnon
• 金额: $ 7万
• 依托单位: Johns Hopkins University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1511185&HistoricalAwards=false
• 关键词: UNS; Non; Optical; Detection; Biomolecular

The requested funding is for instruments that will enable development of medical assays inexpensively and accurately. The approach is new and novel, and has the potential to reduce health care cost.This is a request for two pieces of instrument and equipment that will augment his on going research funded via CAREER. The PI's CAREER proposal was about developing an electrokinetic portable multiplexed biosensing system that would detect biomolecules at liquid-liquid interfaces. The plan is - to understand how biomolecular interactions influence the electrical response of a liquid interface; to understand how the physical characteristics of the liquid interface determine the biosensing specificity and sensitivity; and, to develop multiplexed biosensing capabilities and validate the liquid biosensors for disease biomarkers in practical samples. The core idea is to examine a biomolecular-binding event as a perturbing source interfacial polarization, which we can then be detect by measuring interfacial position. The requested instrument will enable the PI to quantify liquid interfacial position non-invasively in real-time The Broader Impact of the PI's CAREER is to engage awareness in high school students, and via Science Academy program in Baltimore City Middle schools as well as engage underrepresented students in his research lab.

所要求的资金是用于能够开发廉价和准确的医学分析的仪器。这种方法是新颖的，并有可能降低医疗成本。这是一个请求的两件仪器和设备，将加强他正在进行的研究通过职业资助。PI的CAREER提案是关于开发一种电动便携式多路复用生物传感系统，该系统将检测液-液界面处的生物分子。该计划是-了解生物分子相互作用如何影响液体界面的电响应;了解液体界面的物理特性如何决定生物传感的特异性和灵敏度;以及，开发多路复用生物传感能力，并验证液体生物传感器在实际样品中的疾病生物标志物。其核心思想是将生物分子结合事件作为扰动源界面极化进行研究，然后通过测量界面位置来检测。 所要求的仪器将使PI能够实时非侵入性地量化液体界面位置。PI职业生涯的更广泛影响是通过巴尔的摩市中学的科学学院项目，吸引高中生的认识，并吸引他的研究实验室中代表性不足的学生。