UNS: Non-Optical Detection of Biomolecular Binding Events at Electrical Liquid Interfaces

申请的资金将用于能够以低廉和准确的成本开发医学分析的仪器。这种方法是新的和新颖的，并有可能降低医疗保健成本。这是对两件仪器和设备的请求，这将增加他通过职业生涯资助的正在进行的研究。PI的职业计划是关于开发一种电动便携式多路生物传感系统，该系统将检测液-液界面上的生物分子。该计划是-了解生物分子相互作用如何影响液体界面的电响应；了解液体界面的物理特性如何决定生物传感的特异性和灵敏度；以及开发多路生物传感能力并在实际样品中验证用于疾病生物标志物的液体生物传感器。其核心思想是将生物分子结合事件作为微扰源的界面极化，然后我们可以通过测量界面位置来检测。所要求的仪器将使PI能够非侵入性地实时量化液体界面位置PI的职业生涯的更广泛影响是通过巴尔的摩市中学的科学学院计划在高中生中提高认识，以及在他的研究实验室中吸引代表性不足的学生。

The requested funding is for instruments that will enable development of medical assays inexpensively and accurately. The approach is new and novel, and has the potential to reduce health care cost.This is a request for two pieces of instrument and equipment that will augment his on going research funded via CAREER. The PI's CAREER proposal was about developing an electrokinetic portable multiplexed biosensing system that would detect biomolecules at liquid-liquid interfaces. The plan is - to understand how biomolecular interactions influence the electrical response of a liquid interface; to understand how the physical characteristics of the liquid interface determine the biosensing specificity and sensitivity; and, to develop multiplexed biosensing capabilities and validate the liquid biosensors for disease biomarkers in practical samples. The core idea is to examine a biomolecular-binding event as a perturbing source interfacial polarization, which we can then be detect by measuring interfacial position. The requested instrument will enable the PI to quantify liquid interfacial position non-invasively in real-time The Broader Impact of the PI's CAREER is to engage awareness in high school students, and via Science Academy program in Baltimore City Middle schools as well as engage underrepresented students in his research lab.