UNS : Nanoplasmonic interferometric sensor array for dynamic multiplexed functional assay of white blood cells

[12417] Bartoli, Filbert J.研究人员将制造一种新的传感器阵列，用于分析单个细胞上免疫细胞的分泌功能。科学家们相信，这种理解对于理解人体生理学的一个重要组成部分——免疫反应是很重要的。该项目成功完成的影响将是关于免疫细胞行为的新数据，并可能用于了解疾病。本研究将开发一个传感平台，该平台具有在单细胞水平上动态、多路感知免疫细胞分泌功能所需的时间和空间分辨率。知识优势：纳米等离子干涉传感器阵列将在微流控平台的预定位置设计和制造，并仔细对齐功能化表面，用于多路生物分子检测和单细胞的动态多路传感。纳米等离子体干涉传感器的空间分辨率预计将比现有方法提高两个数量级，而在相同的空间分辨率下，其检测极限将比纳米孔阵列等离子体传感器低两个数量级。微流控生物传感平台将与干涉传感器阵列集成，用于监测来自16个lps刺激的巨噬细胞的三种细胞因子（IL-10， MMP-9和ifn - γ）的单细胞动态分泌模式。这些数据有助于理解免疫反应的动力学和异质性。它将促进对免疫功能的基本理解，并可能影响感染、炎症和自身免疫性疾病的新诊断和治疗方法的发展。更广泛的影响：研究和教育的整合是拟议研究的重中之重，这将为本科生和研究生培训提供机会，使他们能够获得跨学科实验室体验的全新仪器。这些研究将有助于新课程的开发，并直接影响到本科课程的设置。调查人员将积极参与正在进行的K-12外展计划，重点关注代表性不足的群体。

1512417 Bartoli, Filbert J. The researchers will fabricate a new sensor array that will analyze secretory function of immune cells at a single cell. It is believed by scientists that such understanding is important in understanding an important part of human physiology, the immune response. The impact of a successful complete of the project will be new data on how immune cells behave, and that could potentially be used in understanding diseases. This research will develop a sensing platform that possesses the temporal and spatial resolution required for dynamic, multiplexed sensing of secretory function of immune cells at the single-cell level. Intellectual Merit: Nanoplasmonic interferometric sensor arrays will be designed and fabricateed at predefined locations in a microfluidic platform, with careful alignment of functionalized surfaces for multiplexed biomolecular detection and for dynamic multiplexed sensing of single cells. Nanoplasmonic interferometric sensors is expected to exhibit two orders of magnitude greater spatial resolution than currentmethods, and two orders of magnitude lower detection limit than nanohole array plasmonic sensors with comparable spatial resolution. The microfluidic biosensing platform will be integrated with the interferometric sensor arrays for monitoring the single cell dynamic secretion pattern of three cytokines (IL-10, MMP-9 and IFN-gamma) from sixteen LPS-stimulated macrophages. These data are useful in the understanding of the dynamics and heterogeneity of immune response. It will advance the fundamental understanding of immunological function, and potentially impact development of new diagnostics and therapeutics for infection, inflammation, and autoimmune disease. Broader Impacts: Integration of research and education is a high priority of the proposed research, which will provide opportunities for undergraduate and graduate student training, giving them access to new entirely instrumentation for interdisciplinary laboratory experiences. The research will contribute to new course development and directly impact the undergraduate curriculum. The investigators will actively participate in ongoing K-12 outreach programs focused on underrepresented groups.