UNS: Mechanisms of UV inactivation of viruses for tailored disinfection applications
UNS:用于定制消毒应用的病毒紫外线灭活机制
基本信息
- 批准号:1512616
- 负责人:
- 金额:$ 33万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-09-01 至 2018-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1512616RodriguezThe goal of this proposal is to gain fundamental insights into the action of ultraviolet light on viruses. The study will examine the molecular level viral responses to ultraviolet irradiation to improve current ultraviolet light disinfection and design of future ultraviolet light technologies. The research aims to answer a number of fundamental questions around virus inactivation with ultraviolet light that can inform engineering practice, public health, and subsequent regulatory policy and thus has broad reaching impacts.The proposed activity will advance knowledge in a number of key areas. New fundamental knowledge will be developed into the molecular-level response of viruses to ultraviolet light irradiation during disinfection practice. Specifically, in addition to adenoviruses, new studies will be performed on another double stranded DNA virus - JC polyomavirus - to test the generalizability of the findings with adenoviruses in response to low-pressure and medium-pressure ultraviolet light. This research will move beyond the traditional evaluations of laboratory strains of adenoviruses and isolate and test environmental strains to determine if there are any differences in the ultraviolet light -response that may be related to acclimated responses (such as DNA repair) from repeated exposure to and propagation in laboratory cell culture lines. A number of new areas in molecular microbiology methods, some of which we have developed, will be exploited including early stage infection inhibition due to specific ultraviolet light wavelengths, DNA repair from trans-lesion synthesis, protein damage assessment using electrophoresis and mass spectrometry techniques, and DNA damage using a new long range PCR technique we recently published. These fundamental findings will be used to develop a tailored wavelength ultraviolet-LED based energy efficient irradiation system to optimize disinfection at lower cost than conventional ultraviolet sources. The work proposed herein will: (1) evaluate inactivation kinetics and wavelength sensitivity of two double stranded DNA viruses, (2) extend the adenovirus studies to adenoviruses isolated from the environment, (3) evaluate the fundamental role of DNA repair in the cell culture infectivity outcomes, (4) incorporate newly developed molecular biology-based assays to assess DNA damage and protein damage from ultraviolet light irradiation at varying wavelengths, and, (5) understand the implications for disinfection using tailored wavelength approaches made possible by the development of nanometer-specific ultraviolet light emitting diodes (LEDs). Regulators and public health officials need information to help make sound decisions for the public sector. The issue of virus disinfection for small systems is currently contentious as the Groundwater Rule specifically forbids the use of UV for any virus inactivation credit due to the high doses required for adenovirus inactivation that cannot currently be field-validated in UV reactors. Therefore, the timing of this research is critical if UV disinfection is going to play any role for virus disinfection in groundwater. Thus, small-systems wanting to use UV for protection of public health, may needlessly suffer.
1512616 Rodriguez该提案的目标是获得紫外线对病毒作用的基本见解。该研究将检查分子水平的病毒对紫外线照射的反应,以改进当前的紫外线消毒和未来紫外线技术的设计。该研究旨在回答围绕紫外线灭活病毒的一些基本问题,这些问题可以为工程实践、公共卫生和随后的监管政策提供信息,从而产生广泛的影响。拟议的活动将在一些关键领域推进知识。新的基础知识将发展到分子水平的病毒响应紫外线照射消毒实践中。具体来说,除了腺病毒之外,还将对另一种双链DNA病毒- JC多瘤病毒进行新的研究,以测试腺病毒对低压和中压紫外线的反应的发现的普遍性。这项研究将超越腺病毒实验室菌株的传统评估,并分离和测试环境菌株,以确定是否有任何差异,在紫外线反应,可能与驯化反应(如DNA修复)从重复暴露于实验室细胞培养系和繁殖。分子微生物学方法的一些新领域,其中一些我们已经开发,将被利用,包括早期感染抑制由于特定的紫外光波长,DNA修复从跨损伤合成,蛋白质损伤评估使用电泳和质谱技术,和DNA损伤使用一个新的远程PCR技术,我们最近出版。这些基本发现将用于开发一种定制的波长紫外线LED为基础的能源效率的照射系统,以优化消毒比传统的紫外线源更低的成本。本文件提议的工作将:(1)评估两种双链DNA病毒的灭活动力学和波长敏感性,(2)将腺病毒研究扩展到从环境中分离的腺病毒,(3)评估DNA修复在细胞培养感染性结果中的基本作用,(4)结合新开发的基于分子生物学的测定来评估来自不同波长的紫外线照射的DNA损伤和蛋白质损伤,以及,(5)了解通过开发纳米特定的紫外发光二极管(LED),使用定制波长方法进行消毒的含义。监管机构和公共卫生官员需要信息来帮助公共部门做出合理的决策。小型系统的病毒消毒问题目前存在争议,因为地下水规则特别禁止使用紫外线进行任何病毒灭活,因为腺病毒灭活所需的高剂量目前无法在紫外线反应器中进行现场验证。因此,如果紫外线消毒将在地下水中的病毒消毒中发挥任何作用,这项研究的时机至关重要。因此,希望使用紫外线保护公众健康的小型系统可能会受到不必要的影响。
项目成果
期刊论文数量(0)
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Roberto Rodriguez其他文献
Process Management in IoT Operating Systems: Cross-Influence between Processing and Communication Tasks in End-Devices
物联网操作系统中的流程管理:终端设备中处理和通信任务之间的交叉影响
- DOI:
10.3390/s19040805 - 发表时间:
2019 - 期刊:
- 影响因子:0
- 作者:
Roberto Rodriguez;Ramiro Utrilla;A. Rozas;Á. Araujo - 通讯作者:
Á. Araujo
Aortic versus axillary cannulation in acute type A aortic dissection repair: A meta-analysis
主动脉插管与腋窝插管在急性 A 型主动脉夹层修复中的比较:一项荟萃分析
- DOI:
10.1177/02184923241232008 - 发表时间:
2024 - 期刊:
- 影响因子:0.7
- 作者:
Yoshiyuki Yamashita;Serge Sicouri;Aleksander Dokollari;Roberto Rodriguez;Scott M. Goldman;B. Ramlawi - 通讯作者:
B. Ramlawi
Randomized Controlled Trial for Microsoft Security Copilot
Microsoft Security Copilot 的随机对照试验
- DOI:
- 发表时间:
2023 - 期刊:
- 影响因子:0
- 作者:
Benjamin G. Edelman;James Bono;Sida Peng;Roberto Rodriguez;Sandra Ho - 通讯作者:
Sandra Ho
TCT-8 Long-Term Outcomes in Patients Undergoing Thoracic Endovascular Aortic Repair: Single Center Experience
TCT-8 接受胸主动脉腔内修复术患者的长期结果:单中心经验
- DOI:
10.1016/j.jacc.2023.09.013 - 发表时间:
2023-10-24 - 期刊:
- 影响因子:22.300
- 作者:
Aleksander Dokollari;Serge Sicouri;Roberto Rodriguez;Robert Meisner;Henry Hirsch;Vincent DiGiovanni;Basel Ramlawi - 通讯作者:
Basel Ramlawi
AORTIC VALVE REPAIR/REPLACEMENT SHORTENS THE PROLONGED QT INTERVAL IN PATIENTS WITH ACQUIRED LONG QT SYNDROME ASSOCIATED WITH AORTIC VALVE STENOSIS
- DOI:
10.1016/s0735-1097(16)30835-x - 发表时间:
2016-04-05 - 期刊:
- 影响因子:
- 作者:
Li Zhang;Amanulla Khaji;Zakir Shaik;Scott Goldman;Roberto Rodriguez;Lisa Igidbashian;Hafiz Khan;Eric Gnall;Paul Coady;Katie Hawthorne;Konstadinos Plestis;Peter Kowey - 通讯作者:
Peter Kowey
Roberto Rodriguez的其他文献
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{{ truncateString('Roberto Rodriguez', 18)}}的其他基金
Collaborative Research: RAPID: Assessment of Water Quality in Puerto Rico after Hurricane Maria
合作研究:RAPID:飓风玛丽亚后波多黎各水质评估
- 批准号:
1812521 - 财政年份:2018
- 资助金额:
$ 33万 - 项目类别:
Standard Grant
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