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RAPID: The membrane-bound structure of fusion loops of the Ebola virus envelope glycoprotein

RAPID：埃博拉病毒包膜糖蛋白融合环的膜结合结构

基本信息

批准号：
1515890
负责人：
Themis Lazaridis
金额：
$ 10.25万
依托单位：
CUNY City College
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2015
资助国家：
美国
起止时间：
2015-01-01 至 2016-12-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1515890&HistoricalAwards=false
关键词：
RAPID membrane bound structure fusion

项目摘要

This NSF Rapid response Research (RAPID) project will support a computational approaches to understand the structure of Ebola virus proteins important in fusing to cells and causing infection. Currently there are no effective treatment method against Ebola outbreaks. If the investigators are successful, results of this proposal would provide a target for a drug design. Once the structure of the target protein is determined, then computational drug screening approaches can be used to examine large databases of compounds that are already available, that could be used to prevent the action of the virus on infected individuals. In this way, new inhibitors to treat the virus can be identified. The researchers will use computational approaches to identify the conformation of the segment of the envelope glycoprotein of Ebola virus that inserts into host membrane and facilitates fusion of the viral membrane with the host membrane enabling the entry of virus into the host cell. This segment of the envelope glycoprotein forms a loop to anchor the virus to host cell membrane. This project is aimed to determine the conformation of this loop in the prehairpin intermediate state, which can be target for designing small molecule and peptide inhibitors as means of preventing the membrane fusion between the virus and the host cell. The work should lead to new targets for drug discovery associated with the treatment and/or prevention of Ebola infection.

NSF的快速反应研究（RAPID）项目将支持一种计算方法，以了解埃博拉病毒蛋白质的结构，这些蛋白质在融合细胞和引起感染方面很重要。目前还没有针对埃博拉疫情的有效治疗方法。如果研究人员成功，这一提议的结果将为药物设计提供一个目标。一旦确定了靶蛋白的结构，就可以使用计算药物筛选方法来检查已经可用的化合物的大型数据库，这些化合物可以用于防止病毒对感染个体的作用。通过这种方式，可以识别治疗病毒的新抑制剂。研究人员将使用计算方法来识别埃博拉病毒包膜糖蛋白片段的构象，该片段插入宿主膜并促进病毒膜与宿主膜的融合，从而使病毒能够进入宿主细胞。这段包膜糖蛋白形成一个环，将病毒锚在宿主细胞膜上。本项目旨在确定该环在前发夹中间状态下的构象，这可以作为设计小分子和肽抑制剂的目标，作为防止病毒与宿主细胞之间的膜融合的手段。这项工作将为与治疗和/或预防埃博拉感染相关的药物发现带来新的目标。