Site-directed spin labeling EPR spectroscopy in vivo

体内定点自旋标记 EPR 光谱

• 批准号: 221211233
• 负责人: Professor Dr. Heinz-Jürgen Steinhoff
• 金额: --
• 依托单位: Arbeitsgruppe Makromolekülstruktur
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/221211233?language=en
• 关键词: Site; directed; spin; labeling; EPR

Site-directed spin labeling (SDSL) EPR spectroscopy has emerged as a powerful technique to study the structure and conformational dynamics of biomolecules in vitro; however, the application of this technique in live cells still remains challenging. The goal of this proposal is the development and application of SDSL EPR spectroscopy in vivo, which allows addressing problems of cell biology not accessible by in vitro experiments. Orthogonal spin labeling techniques including enzymatic labeling and click chemistry approaches, which have been developed in the preceding funding period, will be applied to study the interaction and conformational changes of the bacterial toxin colicin A and the corresponding immunity protein Cai in live Escherichia coli cells.

定点自旋标记（SDSL）EPR光谱已成为一种强大的技术，在体外研究生物分子的结构和构象动力学;然而，这种技术在活细胞中的应用仍然具有挑战性。本提案的目标是开发和应用SDSL EPR光谱在体内，这使得解决问题的细胞生物学无法访问的体外实验。正交自旋标记技术，包括酶标记和点击化学方法，这已经在前一个资助期内开发的，将被应用于研究细菌毒素colicin A和相应的免疫蛋白质Cai在活大肠杆菌细胞中的相互作用和构象变化。