Biological Basis for Methane Synthesis in Oxic Lake Waters

含氧湖水中甲烷合成的生物学基础

• 批准号: 1529461
• 负责人: Timothy McDermott
• 金额: $ 20万
• 依托单位: Montana State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2018-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1529461&HistoricalAwards=false
• 关键词: Biological; Basis; Methane; Synthesis; Oxic

Lakes and rivers are part of the terrestrial landscape, but their contributions to the terrestrial greenhouse gas (GHG) balance have typically not been explicitly included in global GHG budgets. CO2 is the dominant GHG, however CH4 is a more potent long-lived atmospheric GHG, and viewed to account for up to 20-30% of the global warming effect. Atmospheric CH4 levels are on the rise again after a hiatus of several years, yet attributing this rise to specific changes in sources/sinks has so far proved elusive. Accurately predicting how freshwater contributions to the global CH4 budget may be altered by changes to environmental conditions requires a better mechanistic understanding of all the biological processes that form CH4. Research in this project has the potential to contribute to a paradigm shift in our understanding of biological CH4 production. To date, this microbiological activity has been known to be a strictly anaerobic process, yet recent scientific literature has described CH4 synthesis in oxygen saturated aquatic environments. This phenomenon was also observed in Yellowstone Lake, a pristine lake that is largely protected from anthropogenic inputs and thus represents an aquatic system that is natural in composition and function. The research component of this award will focus on identifying the biological basis for CH4 synthesis in an oxic environment, and promises to provide novel contributions to the field of microbial ecology as well as an enhanced understanding of CH4 cycling in the biosphere. This project is collaborative, where both principal investigators will provide state-of-the-art technical guidance to a postdoctoral scholar (human resource development) who will gain experience in both field and lab activities. These students will be recruited from underrepresented groups from the state of Montana (Native Americans). The investigators will continue their broader impacts efforts by reaching out public media and K-12 school teachers.CH4 is a biogenic gas that is an important component of global carbon cycling. While viewed to be produced strictly by methanogens, it nevertheless is found at supersaturating concentrations in oxic marine and freshwaters. Known as the "methane paradox" this has been the focus of significant efforts aimed at understanding its biological basis. Generation by methanogens in benthic environments and aerobic metabolism of methylated compounds may contribute at some level, but cannot account for the bulk of this CH4. Rather, recent reports point to methanogens in oxygen saturated environments, and even more paradoxically, the involvement of oxygenic phototrophs. The preliminary efforts by the investigators on Yellowstone Lake are consistent with these novel observations and imply methanogenesis under oxygen saturating conditions. This proposed research aims to unveil the biology that underlies the paradoxical formation of the pelagic methane enriched zone (PMEZ) in O2 saturated water column of Yellowstone Lake by 1) characterization of methane distribution and cycling rates, and identification of the C substrate involved in methanogenesis; and 2) isolation and characterization of the organisms involved. Research funded by this award promises to shed considerable light on the identity of the underlying biology, interesting microbial synergistic relations and contribute to a more complete understanding of carbon cycling in aquatic environments.

湖泊和河流是陆地景观的一部分，但它们对陆地温室气体平衡的贡献通常没有明确列入全球温室气体预算。 CO2是主要的温室气体，而CH 4是一种更有效的长期大气温室气体，被认为占全球变暖效应的20-30%。大气CH 4水平在中断几年之后再次上升，但迄今为止，将这种上升归因于源/汇的具体变化还很难。要准确预测淡水对全球CH 4收支的贡献如何因环境条件的变化而改变，就需要对形成CH 4的所有生物过程有更好的机械理解。 该项目的研究有可能有助于我们对生物甲烷生产的理解发生范式转变。迄今为止，这种微生物活动被认为是一个严格的厌氧过程，但最近的科学文献描述了在氧饱和的水生环境中甲烷的合成。黄石湖也观察到这种现象，这是一个原始湖泊，基本上不受人为输入的影响，因此代表了一个在组成和功能上都是自然的水生系统。该奖项的研究部分将侧重于确定甲烷合成的生物学基础，并承诺为微生物生态学领域提供新的贡献，以及加强对生物圈中甲烷循环的理解。该项目是合作的，其中两个主要研究者将提供最先进的技术指导，以博士后学者（人力资源开发）谁将获得在现场和实验室活动的经验。这些学生将从蒙大拿州（美洲原住民）代表性不足的群体中招募。研究人员将通过接触公共媒体和K-12学校教师来继续他们更广泛的影响工作。CH 4是一种生物源气体，是全球碳循环的重要组成部分。 虽然被认为是严格由产甲烷菌产生的，但它在好氧的海洋和淡水中以过饱和浓度存在。 这被称为“甲烷悖论”，一直是旨在了解其生物学基础的重大努力的焦点。 底栖环境中的产甲烷菌和甲基化化合物的有氧代谢可能在一定程度上有所贡献，但不能解释这一CH 4的大部分。 相反，最近的报告指出，产甲烷菌在氧饱和的环境中，甚至更矛盾的是，参与产氧光合生物。 研究人员对黄石湖的初步研究与这些新的观察结果一致，并暗示在氧饱和条件下发生甲烷生成。 这项拟议的研究旨在揭示黄石湖O2饱和水柱中浮游甲烷富集区（PMEZ）矛盾形成的生物学基础：1）甲烷分布和循环速率的表征，以及参与甲烷生成的C底物的鉴定; 2）所涉及的生物体的分离和表征。该奖项资助的研究有望揭示潜在生物学的身份，有趣的微生物协同关系，并有助于更全面地了解水生环境中的碳循环。

项目成果

Methylphosphonate metabolism by Pseudomonas sp. populations contributes to the methane oversaturation paradox in an oxic freshwater lake: Methylphosphonate and aquatic CH 4 oversaturation

假单胞菌的甲基膦酸代谢。

• DOI: 10.1111/1462-2920.13747
• 发表时间: 2017
• 期刊: Environmental Microbiology
• 影响因子: 5.1
• 作者: Wang, Qian;Dore, John E.;McDermott, Timothy R.
• 通讯作者: McDermott, Timothy R.