MRI: Development of a High-Resolution Charge Detection Mass Spectrometer with MS/MS Capabilities

在主要研究仪器（MRI）、化学研究仪器和设施（CRIF）以及化学测量和成像（CMI）项目的资助下，印第安纳大学的Martin Jarrold教授将开发一种具有串联功能的高分辨率电荷检测质谱仪（MS/MS）。质谱法是一种强大的分析工具，用于识别混合物或物质的组成。它通过测量组分产生的离子的质量电荷比（m/z）来确定组分的质量。它被广泛用于分析化学、生物和环境样品。将建造第二代电荷检测质谱仪（CD/MS），该质谱仪将纳入性能升级并增加新的功能。质量分辨率将至少提高一个数量级，并且将增加进行质谱联用（其中选定的离子被分解并通过CD/MS进行分析）的能力。开发完成后，该谱仪将用于研究项目，包括研究(a)乙型肝炎病毒组装中的动力学捕获中间体，(B)噬菌体P22原衣壳中的支架蛋白，(c)腺相关病毒中的DNA包装，以及(d)土鼠肝炎病毒（WHV）衣壳自组装中的非二十面体多态性。

With this award from the Major Research Instrumentation (MRI), the Chemistry Research Instrumentation and Facilities (CRIF) and the Chemical Measurement and Imaging (CMI) programs, Professor Martin Jarrold from Indiana University will develop a high resolution charge detection mass spectrometer (MS) with tandem capabilties (MS/MS). Mass spectrometry is a powerful analytical tool used to identify the composition of a mixture or material. It determines the mass of the components by measuring the mass to charge ratio (m/z) of ions produced from the components. It is broadly used to analyze chemical, biological and environmental samples. A second generation charge detection mass spectrometer (CD/MS) will be built which will incorporate performance upgrades and add new capabilities. The mass resolution will be improved by at least an order of magnitude and the ability to perform MS/MS (where selected ions are broken up and analyzed by CD/MS) will be added.Upon development, the spectrometer will be used in research including projects that study (a) kinetically trapped intermediates in the assembly of hepatitis B virus, (b) scaffolding protein in bacteriophage P22 procapsids, (c) DNA packaging in adeno-associated virus, and (d) non-icosahedral polymorphs in the self-assembly of Woodchuck hepatitis virus (WHV) capsids.