Ecological impacts and drivers of double-stranded DNA viral communities in the global oceans

全球海洋中双链 DNA 病毒群落的生态影响和驱动因素

• 批准号: 1536989
• 负责人: Matthew Sullivan
• 金额: $ 85.15万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1536989&HistoricalAwards=false
• 关键词: Ecological; impacts; drivers; double; stranded

Ocean microbes produce half of the oxygen that humans breath and drive much of the energy and nutrient transformations that fuel ocean ecosystems. Viruses of microbes alter these microbial impacts through mortality, moving genes from one organism to another, and reprogramming a host cell's metabolism during infection. However, current understanding of ocean viruses is limited to just a few specific model systems or study sites. This project will produce a foundational dataset - the Global Ocean Virome (GOV) - for contextualizing newly discovered viruses, and use this dataset to evaluate the environmental conditions that structure viral communities or how viral communities change over time and space. Additionally, the project will illuminate 'viral dark matter' by experimentally identifying viral structural proteins and generating and investigating single-cell genomic datasets to link novel and abundant viruses to their host cells. The project will train 6 researchers, as well as lead to curriculum, seminars and a public exhibit at The Wellington School and the Columbus Center Of Science and Industry that will together reach approximately 500 students and 250,000 members of the public.The GOV dataset is comprised of 104 viral metagenomes from around the world's surface and deep oceans. This project seeks to analyze the GOV to identify and quantify viral populations globally, then evaluate these data for ecological patterns to determine the ecological drivers of surface and deep ocean viral community structure. These patterns and drivers will be interpreted in the context of (i) viral metaproteomic experiments to maximally annotate unknown viral proteins that are structural, (ii) paired microbial sequence datasets (metagenomes and metatranscriptomes) that will enable assessment of how biotic factors impact viral community structure and (iii) single-cell amplified genomes and phageFISH experiments that will enable identification of hosts for abundant and novel viruses identified in the GOV. In total, this project will further optimize recently developed genome- and population-based viral ecology methods to establish a first available global map of surface and deep ocean viral populations from both free viromes and infected microbes. These analyses will help evaluate and establish myriad hypotheses about viral roles in marine microbial ecology and biogeochemistry, and the dataset will be a foundational resource for microbial and viral ecologists to contextualize new viruses, identify viruses in microbial datasets, and explore virus-host interactions both phenomenologically and through ecosystem modeling. The GOV will be made publicly available through the NSF-funded iPlant Cyberinfrastructure and metaVIR (http://metavir-meb.univ-bpclermont.fr/).

海洋微生物产生人类呼吸的氧气的一半，并驱动为海洋生态系统提供燃料的大部分能量和营养转化。微生物的病毒通过死亡改变这些微生物的影响，将基因从一个生物体转移到另一个生物体，并在感染期间重新编程宿主细胞的代谢。然而，目前对海洋病毒的了解仅限于几个特定的模型系统或研究地点。该项目将产生一个基础数据集-全球海洋病毒组（GOV）-用于将新发现的病毒置于背景中，并使用该数据集评估构建病毒群落的环境条件或病毒群落如何随时间和空间变化。此外，该项目还将通过实验识别病毒结构蛋白，并生成和研究单细胞基因组数据集，将新型和丰富的病毒与其宿主细胞联系起来，从而阐明“病毒暗物质”。该项目将培训6名研究人员，并在惠灵顿学校和哥伦布科学与工业中心举办课程、研讨会和公开展览，这些活动将共同吸引大约500名学生和25万名公众。GOV数据集由来自世界各地的表面和深海的104个病毒宏基因组组成。该项目旨在分析GOV以识别和量化全球病毒种群，然后评估这些数据的生态模式，以确定表面和深海病毒群落结构的生态驱动因素。这些模式和驱动因子将在以下背景下进行解释：（i）病毒元蛋白质组学实验，以最大限度地注释结构上未知的病毒蛋白质，（ii）配对的微生物序列数据集（宏基因组和元转录组），这将使生物因素如何影响病毒群落结构的评估和（iii）单-细胞扩增的基因组和噬菌体FISH实验，这将能够鉴定GOV中鉴定的大量和新型病毒的宿主。总之，该项目将进一步优化最近开发的基于基因组和种群的病毒生态学方法，以建立第一个可用的全球地图，显示来自游离病毒组和受感染微生物的表层和深海病毒种群。这些分析将有助于评估和建立关于病毒在海洋微生物生态学和海洋地球化学中的作用的无数假设，并且数据集将成为微生物和病毒生态学家的基础资源，以了解新病毒，识别微生物数据集中的病毒，并通过现象学和生态系统建模探索病毒-宿主相互作用。GOV将通过NSF资助的iPlant Cyberinfrastructure和MetaVIR（http：//metavir-meb.univ-bpclermont.fr/）公开提供。

项目成果

Marine DNA Viral Macro- and Microdiversity from Pole to Pole

• DOI: 10.1016/j.cell.2019.03.040
• 发表时间: 2019-05-16
• 期刊: CELL
• 影响因子: 64.5
• 作者: Gregory, Ann C.;Zayed, Ahmed A.;Sullivan, Matthew B.
• 通讯作者: Sullivan, Matthew B.