RUI: Growing Up on Ice: Physiological Adaptations and Developmental Plasticity in Weddell Seal Pups Across Two Extreme Physical Environments

RUI：冰上成长：威德尔海豹幼崽在两种极端物理环境中的生理适应和发育可塑性

• 批准号: 1543539
• 负责人: Heather Liwanag
• 金额: $ 101.59万
• 依托单位: California Polytechnic State University Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-15 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1543539&HistoricalAwards=false
• 关键词: RUI; Growing; Up; Ice; Physiological

The transition of young from parental care to independence is a critical stage in the life of many animals. Surviving this stage can be especially challenging for polar mammals where the extreme cold requires extra energy to keep warm, rather than using the majority of energy for growth, development and physical activities. Young Weddell seals (Leptonychotes weddellii) have only weeks to develop the capabilities to survive both on top of the sea ice and within the -1.9°C seawater where they can forage for food. The project seeks to better understand how Weddell seal pups rapidly develop (within weeks) the capacity to transition between these two extreme environments (that differ greatly in their abilities to conduct heat) and how they budget their energy during the transition. Though the biology and physiology of adult Weddell seals is well studied, the energetic and physiological strategies of pups during development is still unclear. Understanding factors that may affect survival at critical life history events is essential for better understanding factors that might affect marine mammal populations. Weddell seals are the southernmost breeding mammal and are easily recognizable as quintessential Antarctic seals. Determining potential vulnerabilities at critical life stages to change in the Antarctic environment will facilitate the researchers' ability to not only gain public interest but also communicate how research is revealing ways in which changes are occurring at the poles and how these changes may affect polar ecosystems. By collaborating with the Marine Mammal Center, the project will directly reach the public, through curricular educational materials and public outreach that will impact over 100,000 visitors annually.To elucidate the physiological strategies that facilitate the survival of Weddell seal pups from birth to independence, the proposed study examines the development of their thermoregulation and diving capability. To achieve this, the project will determine the mechanisms that Weddell seal pups use to maintain a stable, warm body temperature in air and in water and then examine the development of diving capability as the animals prepare for independent foraging. The researchers will take a fully integrative approach- making assessments from proteins to tissues to the whole-animal level- when investigating both these objectives. To assess the development of thermoregulatory capability, researchers will quantify body insulation, resting metabolic rates in air and in water, muscle thermogenesis (shivering), and body surface temperatures in the field. The project will also assess the development of dive capability by quantifying oxygen storage capacities and measuring early dive behavior. To identify possible cellular mechanisms for how Weddell seals navigate this trade-off during development, the program will quantify several key developmental regulators of increased hypoxic capacity (HIF, VEGF and EPO) using qPCR, as well as follow the proteomic changes of adipose and muscle tissue, which will include abundance changes of metabolic, antioxidant, cytoskeletal, and Ca2+-regulating proteins. The study of the physiological development leading up to the transition to independence in pinnipeds will help researchers better predict the effects of climate change on the distribution and abundance of this species and how this will affect other trophic levels. Environmental changes that alter habitat suitability have been shown to decrease population health, specifically because of declines in juvenile survival.

在许多动物的一生中，幼崽从父母的照顾到独立的过渡是一个关键阶段。对于极地哺乳动物来说，生存在这一阶段尤其具有挑战性，因为极端寒冷需要额外的能量来保暖，而不是将大部分能量用于生长、发育和身体活动。年幼的威德尔海豹（Leptonychotes weddellii）只有几周的时间来培养在海冰顶部和-1.9°C的海水中生存的能力，在那里它们可以觅食。该项目旨在更好地了解威德尔海豹幼崽是如何（在几周内）迅速发展出在这两种极端环境（它们传导热量的能力有很大差异）之间转换的能力，以及它们在过渡期间如何分配能量。虽然成年威德尔海豹的生物学和生理学研究得很好，但幼崽在发育过程中的能量和生理策略仍不清楚。了解在关键生活史事件中可能影响生存的因素对于更好地了解可能影响海洋哺乳动物种群的因素至关重要。威德尔海豹是最南端的繁殖哺乳动物，很容易被识别为典型的南极海豹。确定南极环境变化的关键生命阶段的潜在脆弱性，将促进研究人员的能力，不仅能获得公众的兴趣，还能交流研究如何揭示极地发生变化的方式，以及这些变化如何影响极地生态系统。通过与海洋哺乳动物中心的合作，该项目将直接接触公众，通过课程教育材料和公众宣传，每年将影响超过10万名游客。为了阐明威德尔海豹幼崽从出生到独立生存的生理策略，本研究考察了它们的体温调节和潜水能力的发展。为了实现这一目标，该项目将确定威德尔海豹幼崽在空气和水中保持稳定、温暖体温的机制，然后在动物准备独立觅食时检查潜水能力的发展。在研究这两个目标时，科学家们将采取一种完全综合的方法——从蛋白质到组织再到整个动物水平进行评估。为了评估体温调节能力的发展，研究人员将量化身体绝缘、空气和水中的静息代谢率、肌肉产热（颤抖）和体表温度。该项目还将通过量化氧气储存能力和测量早期潜水行为来评估潜水能力的发展。为了确定威德尔海豹在发育过程中如何进行这种权衡的可能细胞机制，该计划将使用qPCR量化几种增加缺氧能力的关键发育调节因子（HIF， VEGF和EPO），并跟踪脂肪和肌肉组织的蛋白质组学变化，其中包括代谢，抗氧化，细胞骨架和Ca2+调节蛋白的丰度变化。对鳍足类动物向独立过渡的生理发育的研究将有助于研究人员更好地预测气候变化对该物种分布和丰度的影响，以及这将如何影响其他营养水平。改变栖息地适宜性的环境变化已被证明会降低种群健康，特别是由于幼鱼存活率下降。