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Evolutionary potential in functional traits of a wetland macrophyte (Juncus effusus) relevant for natural degradation of contaminants

与污染物自然降解相关的湿地大型植物（灯心草）功能性状的进化潜力

基本信息

批准号：
222302821
负责人：
Dr. Stefan Michalski
金额：
--
依托单位：
Institut für Informatik
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2012
资助国家：
德国
起止时间：
2011-12-31 至 2016-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/222302821?language=en
关键词：
Evolutionary potential functional traits wetland

项目摘要

An important ecosystem service of wetland ecosystems is the natural degradation and retention of contaminants. The remediation potential of plants and associated microorganisms is used also as cost-effective and sustainable biotechnology. Hence, for many plant species, functional traits with relevance for the degradation of contaminants are well characterized. However, species are mostly treated as constant entities, neglecting intraspecific genetic variation and resulting in a significant gap in the understanding of the microevolutionary background for this ecosystem service. Applying a combination of molecular and quantitative genetics, the project aims to quantify intra-specific variability in functional traits relevant for the degradation of nitrogen compounds, to investigate its genetic basis and its response to selection. A representative wetland plant (Juncus effusus) will be established as a genetic model system by characterizing its root transcriptome and identifying alleles in putative candidate genes relevant for nitrogen degradation. Furthermore, natural populations of the species along a gradient in concentration of nitrogen compounds will be studied by two approaches. First, nonneutral variation in putative candidate genes will be assessed and the observed patterns will be compared with that of neutral genetic markers and environmental conditions. Second, it will be tested for local adaptation and differentiation in quantitative traits relevant for the degradation potential, such as uptake of nitrogen compounds, root morphology, decomposability of plant biomass and composition of the microbial community in the rhizosphere by reciprocal transplant and common garden experiments. The project will help to understand how intra-specific genetic variability affects a community based regulating ecosystem service.

湿地生态系统的一个重要生态系统服务是污染物的自然降解和保留。植物和相关微生物的补救潜力也被用作具有成本效益和可持续的生物技术。因此，对于许多植物物种来说，与污染物降解相关的功能性状得到了很好的表征。然而，物种大多被视为恒定的实体，忽略了种内的遗传变异，并导致一个显着的差距，在理解微进化的背景下，这种生态系统服务。该项目将分子遗传学和数量遗传学相结合，旨在量化与氮化合物降解有关的功能性状的种内变异，以研究其遗传基础及其对选择的反应。一个具有代表性的湿地植物（灯心草）将被建立作为一个遗传模型系统，其根转录组的特征和识别等位基因在假定的候选基因相关的氮降解。此外，自然种群的物种沿着梯度浓度的氮化合物将研究两种方法。首先，将评估假定候选基因的非中性变异，并将观察到的模式与中性遗传标记和环境条件进行比较。第二，将通过相互移植和普通花园实验，测试与降解潜力有关的数量性状的当地适应性和差异，如氮化合物的吸收、根系形态、植物生物量的可分解性和根际微生物群落的组成。该项目将有助于了解种内遗传变异如何影响基于社区的调节生态系统服务。