Collaborative Research: IDBR Type A: QSTORM-AO-Wavefront-shaping light-sheet microscopy with photoswitchable quantum dots for superresolution imaging in thick tissue

授予格鲁吉亚大学一个奖项，以开发一种用于生物研究的新型超分辨率显微镜。对于学生和早期职业研究人员来说，这种在生物学，物理学，光学和化学工程交叉点的密切合作将提供宝贵的培训经验，并将通过跨学科科学交流技能的专业发展来丰富。所有团队成员将致力于开发在线团队博客，实验室笔记本和公共网站，这些网站将在更广泛的历史，科学和视觉背景下捕捉研究进展，由波士顿科学博物馆策划。科学博物馆团队还将向数千名科学博物馆参观者、学生和更广泛的社区传播这项工作，包括现场演示、演示、播客和一部NOVA风格的短片，以更清晰、更深入地了解生命的奥秘。这部电影将由一位屡获殊荣的NOVA制片人执导。这台仪器将结合使用高分辨率空间光调制器的联合收割机全息成像和校正厚生物组织中光散射引起的失真的新技术，以实现对现代生物研究重要的生物体内分子尺度的分辨率，如果蝇和斑马鱼。在俄亥俄州州立大学，半导体纳米技术将与DNA“折纸”相结合，创造出非常明亮和可切换的光发射器，可单独瞄准生物系统内运行的选定分子。 这种高精度技术的新组合将为研究人员提供重要的亚细胞生物过程的前所未有的视角，例如完整生物体中的神经元信号传导，可能有助于分子和系统生物学，生物工程，医学和人类健康领域的突破。

An award is made to the University of Georgia to develop a novel super-resolution microscope for biological research. For students and early-career researchers, this close collaboration at the intersection of biology, physics, optics, and chemical engineering will provide an invaluable training experience, and it will be enriched with professional development in interdisciplinary science communication skills. All team members will contribute to the development of an online team blog, lab notebook, and public website that will capture the progress of the research within a broader historical, scientific, and visual context, curated by the Boston Museum of Science. The Museum of Science team will also disseminate this work to thousands of science museum visitors, students, and the broader community with live presentations, demonstrations, podcasts, and a short NOVA-style film on the quest to see more clearly and deeply into the mysteries of life. The film will be directed by a multiple award-winning NOVA producer.This instrument will combine holographic imaging using high resolution spatial light modulators and new techniques for correcting distortion induced by light scattering in thick biological tissue to achieve molecular-scale resolution inside organisms important for modern biological research, such as fruit flies and zebrafish. At The Ohio State University, semiconductor nanotechnology will be combined with DNA origami to create intensely bright and switchable light emitters, individually targetable to selected molecules operating within biological systems. This novel combination of high-precision technologies will provide investigators an unprecedented view of important subcellular biological processes, such as neuronal signaling in intact organisms, potentially contributing to breakthroughs in areas of molecular and systems biology, biological engineering, medicine, and human health.