Synoptic analysis of adaptive immune reactions in very early multiple sclerosis: Repertoires and target antigens of B and T cells (A05)

为了探讨多发性硬化症(MS)的早期免疫学变化，我们将平行比较不符合多发性硬化症(MS)的单卵双胞胎(一例亚临床放射隔离综合征(RIS)和另一例确诊多发性硬化症(MS))的CD4+和CD8+T细胞的谱系和靶抗原，以及产生Ig G和Ig M的B细胞，通过使用下一代测序、单细胞分析和重组抗体和T细胞受体，我们希望在非常早期就获得对适应性免疫系统不同组成部分的全面、概要的看法。

To investigate very early immunological changes of multiple sclerosis (MS), we will compare, in parallel, the repertoires and target antigens of CD4+ and CD8+ T cells, as well as IgG- and IgM-producing B cells in selected monozygotic twins who are discordant for MS, i.e., one twin has subclinical radiologically isolated syndrome (RIS) and the other established MS. By employing next generation sequencing, single-cell analysis, and recombinant antibodies and T cell receptors, we hope to obtain a comprehensive, ¿synoptic¿ view of different components of the adaptive immune system in very early as compared to established MS.