PFI:AIR-TT: Preclinical evaluation of bioactive tailored amorphous multiporous (TAMP) powder for the treatment of dentin hypersensitivity

这个PFI：空气技术翻译项目专注于翻译最近开发的用于修复受损牙本质和牙髓组织的技术，这是治愈牙本质过敏症所需的技术。牙病是一种牙齿疾病，在大多数人一生中的某个时候会造成严重的疼痛。目前可用的产品只尝试暂时治疗症状，没有持久的解决问题的方法。本项目致力于纳米微孔玻璃的制备技术，并将一类新型生物活性材料应用于牙科组织的再生。由此产生的玻璃具有定制的无定形多孔(TAMP)结构，促进了骨骼和软组织的生长。该项目将产生新的夯实组合物，并建立其潜在的再生受损的牙本质和牙髓组织，以治愈牙周炎。选定的夯实粉末由相互连接的纳米宏观孔隙率组成，可以根据需要的表面积进行定制，具有从源头上解决问题的独特潜力。因此，当放置在体内时，适当成分的捣固粉可以以受控的方式提供治疗离子，提供一个有望堵塞牙本质小管的微环境，以比目前可用的产品更有效地阻止疼痛症状。更重要的是，新的捣固粉还有望刺激细胞的组织再生，从而获得持久的治愈。相比之下，在这个市场领域，目前的产品可以在柜台上买到或通过处方购买，不会试图再生受损的组织。该项目解决了以下技术差距(S)，因为它从研究发现转化为商业应用。一种基于硅酸钙的简单夯实粉末在实验室实验中显示了骨形成细胞的增殖，在动物体内实验中也显示了骨和软组织的再生。然而，其再生牙齿组织(牙本质和牙髓)的能力一直缺乏证明。该项目试图获得夯实粉末用于牙齿组织再生的治疗能力的证据，然后为临床试验做准备。它还在开发新的成分，将不同治疗方法的好处结合到一个产品中。例如，在硅酸盐玻璃的分子结构中嵌入氟离子、锌离子和钾离子的新型捣固粉正在被制造出来，以在水解酶部位提供持久的氟离子供应，促进再生的锌离子，以及使痛觉神经减敏的钾离子。此外，正在进行测试，以评估牙髓干细胞的体外分化，以及在小鼠模型中植入的夯实硅酸盐上牙髓-牙本质复合体的体内形成。这项从研究发现到商业现实的技术转化是由一位玻璃科学家领导的团队努力完成的，他在之前的NSF支持的研究下开发了捣固物制造技术组合，还有一名具有牙齿组织再生专业知识的牙髓医生、一名具有夯实材料细胞响应专业知识的细胞生物学家，以及一名具有生物医学产品早期商业化经验的医疗行业专家。在其课程期间，该项目直接培训三名研究生进行应用启发的研究，并通过结合材料工程、细胞生物学和牙科科学的跨学科方法解决实际问题。创新的夯实技术及其潜在的社会影响的结合吸引了许多学生的普遍兴趣，这一兴趣将通过该项目更明确的结果进一步扩大。

This PFI: AIR Technology Translation project focuses on translating recently developed technology for the regeneration of damaged dentin and pulp tissue, which is needed for the cure of dentin hypersensitivity (DH). DH is a dental condition that inflicts severe pain in majority of people sometime during their lifetime. The currently available products attempt only to treat the symptoms temporarily, and there is no lasting cure of the problem. This project focuses on nano-macro porous glass fabrication technology and applying a new class of bioactive materials to the regeneration of dental tissue. The resulting glasses, which have tailored amorphous multiporous (TAMP) structure, promote the growth of bone as well as soft tissue. The project will result in new TAMP compositions and establish their potential for the regeneration of damaged dentin and pulp tissue to cure DH. The selected TAMP powders that comprise of interconnected nano-macro porosity, which can be tailored to desired surface area, have the unique potential to cure the problem at its source. Consequently, when placed inside the body, a TAMP powder of appropriate composition can supply therapeutic ions in a controlled manner, providing a microenvironment that is expected to occlude dentin tubules to stop the painful symptoms more effectively than currently available products. More importantly, the new TAMP powders are also expected to stimulate tissue regeneration by the cells for a lasting cure. In contrast, the present products in this market space, available over the counter or by prescription, make no attempt to regenerate damaged tissue. The project addresses the following technology gap(s) as it translates from research discovery toward commercial application. A simple TAMP powder based on calcium silicate has shown proliferation of bone forming cells in laboratory tests and also regeneration of bone and soft tissue regeneration under in vivo tests in animals. However, a demonstration of its ability to regenerate dental tissue (dentin and pulp) has been lacking. The project attempts to obtain evidence for the therapeutic capabilities of TAMP powder for dental tissue regeneration, and thereafter prepare it for clinical trials. It is also developing new compositions that combine the benefits of different treatments in one product. For example, novel TAMP powders with fluoride, zinc and potassium ions embedded within the molecular structure of silicate glass are being fabricated, to provide a lasting supply of fluoride ions at the site of DH, zinc ions that promote regeneration, and potassium ions that desensitize pain-sensing nerves. Further, tests are being performed to assess in vitro differentiation of dental pulp stem cells, and in vivo formation of pulp-dentin complex on TAMP silicates implanted in a mouse model. With this knowledge compositions are optimized for more adherent and faster developing occlusion layer as well as pulp-dentin tissue regeneration.This technology translation from research discovery toward commercial reality is a team effort led by a glass scientist who developed the TAMP fabrication technology portfolio under prior NSF supported research, together with an endodontist with expertise in dental tissue regeneration, a cell biologist with expertise in cell response to TAMP materials, and a healthcare industry expert with experience in the early stage commercialization of biomedical products. During its course the project is directly training three graduate students in use-inspired research and practical problem-solving through a cross-disciplinary approach that combines materials engineering, cell biology and dental science. The combination of innovative TAMP technology and its potential societal impact has drawn much general student interest, which will be further expanded through more definitive results of this project.