Multimodal signal amplification by collaborative plasmonic intensification and catalytic multiplication (c-PI/CM)

非常灵敏的体液中疾病指示分子的测量对于提供高质量的医疗保健至关重要。该项目旨在开发一种既灵敏又可靠的新方法。这种新方法是基于光激发电子振荡和化学反应同时发生在特定纳米结构上的协同作用。在生物传感中，未满足的需求是超灵敏检测（如聚合酶链反应，PCR）和准确定量（如酶联免疫吸附测定，ELISA）。这种能力对于无法进行pcr扩增或受时间、样品数量或资源限制的蛋白质生物标记物是非常需要的。为了满足这一需求，提出了一种通过协同等离子体增强和催化倍增来放大多模态信号的新概念（c-PI/CM）。核心的知识价值是利用PI和CM之间的协作协同作用。协作PI/CM不是简单地通过一种模式增强另一种模式，而是两种模式之间的协作和积极强化。因此，PI/CM与等离子体增强ELISA有本质的不同。为了验证这一假设，PI将设计一系列实验，通过研究c-PI/CM在纳米光刻制备的标准等离子体纳米结构上的相互作用，来阐明c-PI/CM的基本机制。第二个假设是测试c-PI/CM是否会在最近开发的纳米孔金（NPG）磁盘上表现更好，该磁盘具有内在的催化功能，高密度，三维分布的等离子体场增强位点（也称为热点），数量级更大的表面积，以及出色的结构完整性和环境稳定性，所有这些都指向更合理的高性能c-PI/CM平台。几种原位监测技术将用于评估传感性能：LSPR位移，表面增强拉曼散射（SERS）和表面增强荧光（SEF）。

PI: Shih, Wei-Chuan Proposal No: 1605683Very sensitive measurement of disease-indicating molecules in body fluids is essential for delivering quality healthcare. This project aims to develop a new approach that is expected to be both sensitive and reliable. The new approach is based on synergy of light-excited electron oscillation and chemical reaction occuring simultaneously on specialized nanostructures. In biosensing, an unmet need is ultrasensitive detection (like polymerase chain reaction, PCR) with accurate quantitation (like enzyme-linked immunosorbent assay, ELISA). This ability is sorely needed for protein biomarkers which cannot be PCR-amplified, or constrained by time, sample quantity, or resources. To meet this need, a novel concept of multimodal signal amplification by collaborative plasmonic intensification and catalytic multiplication (c-PI/CM) is proposed. The central intellectual merit is to exploit the collaborative synergy between PI and CM. Collaborative PI/CM is not simply enhancing one modality by the other, but the collaboration and positive reinforcement between the two modalities. Thus, PI/CM is fundamentally different from, for example, plasmon-enhanced ELISA. To test this hypothesis, the PI will design a series of experiments to elucidate the fundamental mechanisms of c-PI/CM by investigating their interplay on standard plasmonic nanostructures fabricated by nanolithography. A second hypothesis is to test whether c-PI/CM would perform better on recently developed nanoporous gold (NPG) disks which exhibits intrinsic catalytic functions, high-density, three dimensionally distributed plasmonic field enhancement sites (also known as, hot spots), order-of-magnitude larger surface area, and excellent structural integrity and environmental stability, all pointing to a more plausible high-performance c-PI/CM platform. Several in situ monitoring techniques will be used to assess sensing performance: LSPR shift, surface-enhanced Raman scattering (SERS), and surface-enhanced fluorescence (SEF).