Multimodal signal amplification by collaborative plasmonic intensification and catalytic multiplication (c-PI/CM)

建议编号：1605683非常灵敏地测量体液中的疾病指示分子对于提供高质量的医疗保健是必不可少的。该项目旨在开发一种预计既敏感又可靠的新方法。这种新的方法是基于光激发电子振荡和化学反应在特殊纳米结构上同时发生的协同作用。在生物传感方面，一个尚未得到满足的需求是超灵敏的检测(如聚合酶链式反应，PCR)和准确的定量(如酶联免疫吸附试验，ELISA)。对于不能用PCR扩增或受时间、样本量或资源限制的蛋白质生物标记物来说，这种能力是非常必要的。为了满足这一需求，提出了协同等离子体增强催化倍增多峰信号放大(c-PI/CM)的新概念。核心的智力优势是利用PI和CM之间的协作协同作用。协作性的PI/CM不是简单地加强一种模式和另一种模式，而是两种模式之间的合作和积极的加强。因此，PI/CM从根本上不同于例如血浆酶增强的酶联免疫吸附试验。为了验证这一假设，PI将设计一系列实验，通过研究c-PI/CM与标准等离子体纳米结构的相互作用来阐明c-PI/CM的基本机制。第二个假设是测试c-PI/CM在最近开发的纳米多孔金(NPG)磁盘上是否会表现得更好，这些磁盘具有内在的催化功能、高密度、三维分布的等离子激元增强部位(也称为热点)、数量级更大的表面积、以及优异的结构完整性和环境稳定性，所有这些都指向更合理的高性能c-PI/CM平台。几种现场监测技术将用于评估传感性能：LSPR漂移、表面增强拉曼散射(SERS)和表面增强荧光(SEF)。

PI: Shih, Wei-Chuan Proposal No: 1605683Very sensitive measurement of disease-indicating molecules in body fluids is essential for delivering quality healthcare. This project aims to develop a new approach that is expected to be both sensitive and reliable. The new approach is based on synergy of light-excited electron oscillation and chemical reaction occuring simultaneously on specialized nanostructures. In biosensing, an unmet need is ultrasensitive detection (like polymerase chain reaction, PCR) with accurate quantitation (like enzyme-linked immunosorbent assay, ELISA). This ability is sorely needed for protein biomarkers which cannot be PCR-amplified, or constrained by time, sample quantity, or resources. To meet this need, a novel concept of multimodal signal amplification by collaborative plasmonic intensification and catalytic multiplication (c-PI/CM) is proposed. The central intellectual merit is to exploit the collaborative synergy between PI and CM. Collaborative PI/CM is not simply enhancing one modality by the other, but the collaboration and positive reinforcement between the two modalities. Thus, PI/CM is fundamentally different from, for example, plasmon-enhanced ELISA. To test this hypothesis, the PI will design a series of experiments to elucidate the fundamental mechanisms of c-PI/CM by investigating their interplay on standard plasmonic nanostructures fabricated by nanolithography. A second hypothesis is to test whether c-PI/CM would perform better on recently developed nanoporous gold (NPG) disks which exhibits intrinsic catalytic functions, high-density, three dimensionally distributed plasmonic field enhancement sites (also known as, hot spots), order-of-magnitude larger surface area, and excellent structural integrity and environmental stability, all pointing to a more plausible high-performance c-PI/CM platform. Several in situ monitoring techniques will be used to assess sensing performance: LSPR shift, surface-enhanced Raman scattering (SERS), and surface-enhanced fluorescence (SEF).