Positioning, association and maturation of subunits during 20S proteasome assembly in pro- and eukaryotes (A02)

蛋白质水解降解是一个重要的细胞过程，主要由20S蛋白酶体核心颗粒（CP）完成。仔细调节CPs的组装对于确保每个亚单位的正确定位和功能至关重要。我们提出的A02项目将发现早期和晚期蛋白酶体组装中间体的亚基定位和构象开关，这将有助于更好地理解从原核生物到真核生物的复杂蛋白酶体生物发生。

Proteolytic degradation is an essential cellular process, which is primarily carried out by the 20S proteasome core particle (CP). Carefully regulated assembly of CPs is essential to ensure correct positioning and functioning of each subunit. Our proposed project A02 will discover subunit positioning and conformational switches in early and late proteasome assembly intermediates, which will contribute to a better understanding of sophisticated proteasome biogenesis from prokaryotes to eukaryotes.