Exploiting the Bifunctional Properties of Zinc Oxide as a Smart Biomimetic Material

非技术性：该奖项由材料研究部的生物材料项目授予新泽西理工学院，旨在研究一种用于组织工程应用的智能材料。该奖项由材料研究部的BioMaPS基金和化学，生物工程，环境和运输系统部的生物医学工程项目共同资助。氧化锌具有机电特性，是一种生长因子模拟物，将被制成一种机械柔性的聚合物复合支架，以促进干细胞分化和软骨修复的组织生长。骨关节炎是一种使人衰弱的退行性关节疾病，其中软骨和底层骨组织受损，并可随时间恶化。目前还没有技术可以恢复完全功能的软骨组织。氧化锌支架将被制造并充分表征其双功能特性。间充质干细胞分化为软骨和骨细胞将在体外支架上进行评价。拟议的工作将对可能的组织再生和修复智能材料领域产生重大影响。这里的研究工作也将在教学，培训和教育中传播。主要研究者的实验室通过各种社区和大学方案，积极参与培训、指导和招聘初中一级代表性不足的少数民族和女学生，这些活动因该奖项而得到加强。技术：本项目将研究用于组织工程策略的双功能智能材料。氧化锌（ZnO），这是压电和生长因子模拟物，将被利用，为第一次，其功能特性在组织工程应用。ZnO将以复合形式与聚己内酯（PCL）一起用作支架，以促进干细胞分化。将讨论两个具体目标。该奖项的目的1是制造和充分表征ZnO-聚合物复合支架的压电性能。 ZnO纳米颗粒将与PCL（一种缓慢降解的生物材料）结合，形成复合纤维支架，并测定氧化锌的控释/溶解。复合材料还将在更接近模拟生物环境的条件下表征电输出。然后，压电支架将被表征为局部纳米级机电行为以及本体性质，以将生物反应与机电活性相关联。该奖项的第二个目的是研究间充质干细胞在ZnO-PCL复合支架上体外成骨和软骨分化。本研究基于以下假设：使用具有与天然细胞外基质相似的物理化学性质的纤维支架将刺激间充质干细胞的分化。因此，除了开发用于修复软骨缺损的新型组合疗法外，本研究还将提高对压电或机电效应对细胞分化的作用的科学理解，以及在软骨修复和再生中的潜在应用。在教学、培训和外联活动方面，研究人员将在该奖项支持的研究活动中招募和指导代表性不足的少数群体和妇女。

Nontechnical: This award by the Biomaterials program in the Division of Materials Research to the New Jersey Institute of Technology is to investigate a smart material for tissue engineering applications. This award is co-funded by BioMaPS funds in the Division of Materials Research, and the Biomedical Engineering program in the Division of Chemical, Bioengineering, Environmental, and Transport Systems. Zinc oxide, which has electromechanical properties and is a growth factor mimetic, will be fabricated into a mechanically flexible, polymer composite scaffold to promote stem cell differentiation and tissue growth for cartilage repair. Osteoarthritis is a debilitating, degenerative joint disease where cartilage and the underlying bone tissue becomes damaged and can worsen with time. No technology exists that can restore fully functional cartilage tissue. Zinc oxide scaffolds will be fabricated and fully characterized for their bifunctional properties. Mesenchymal stem cell differentiation into cartilage and bone cells will be evaluated on the scaffolds in vitro. The proposed work would have a significant impact on the fields of smart materials for possible tissue regeneration and repair. The research efforts here also will be disseminated in teaching, training, and education. The principal investigator's laboratory is actively involved in the training, mentoring and recruitment of underrepresented minority and female students starting at the junior high school level through various community and university programs, and these activities are enhanced and strengthened with this award. Technical: This project will investigate a bifunctional smart material for tissue engineering strategies. Zinc oxide (ZnO), which is piezoelectric and a growth factor mimetic, will be utilized, for the first time, for its functional properties in tissue engineering applications. ZnO will be used in composite form with polycaprolactone (PCL) as a scaffold to promote stem cell differentiation. Two specific aims will be addressed. Aim 1 of this award is to fabricate and fully characterize the piezoelectric properties of ZnO-polymer composite scaffolds. Nanoparticles of ZnO will be combined with PCL, a slow-degrading biomaterial, to form composite fibrous scaffolds, and controlled release/dissolution of zinc oxide will be determined. Composites will also be characterized for electrical output in conditions that more closely mimic a biological setting. The piezoelectric scaffolds will then be characterized for localized nanoscale electromechanical behavior as well as bulk properties in order to correlate biological response with electromechanical activity. The second aim of this award is to investigate the osteogenic and chondrogenic differentiation of mesenchymal stem cells on the ZnO-PCL composite scaffold in vitro. This study is based on the hypothesis that the use of a fibrous scaffold having similar physicochemical properties as the native extracellular matrix will stimulate the differentiation of msenchymal stem cells. Therefore, in addition to developing a novel combination therapy for the repair of cartilage defects, this study will enhance the scientific understanding of the role of piezoelectric or electromechanical effects on cell differentiation, and potential applications in cartilage repair and regeneration. With respect to teaching, training and outreach activities, the researchers will recruit and mentor underrepresented minorities and women in the research activities supported by this award.