A Biologically Inspired Material for Stem-Cell Induced Cartilage Repair

用于干细胞诱导软骨修复的生物启发材料

• 批准号: 1207173
• 负责人: Treena Livingston
• 金额: $ 33万
• 依托单位: New Jersey Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1207173&HistoricalAwards=false
• 关键词: Biologically; Inspired; Material; Stem; Cell

This award by the Biomaterials program in the Division of Materials Research to New Jersey Institute of Technology will investigate a novel, semi-synthetic derivative of cellulose, which is one of the most abundant natural materials, for use as a tissue engineering scaffold for cartilage repair. Articular cartilage has a limited capacity to heal. Specialized constructs incorporating cells as well as materials that more closely mimic the structure of the cartilage extracellular matrix during early development may be a promising strategy for promoting the formation of functional cartilage tissue. Sodium cellulose sulfate is water soluble and mimics the structure of glycosaminoglycans. It is a linear polysaccharide that can be synthesized with varying degrees of sulfation for improved bioactivity. Sodium cellulose sulfate could impart functional qualities that are similar to glycosaminoglycans, direct chondrogenesis and cartilage tissue formation. Sodium cellulose sulfate will be combined with gelatin in a fibrous form to more closely mimic the natural extracellular matrix of cartilage in both structure and function. The first aim of the project will be the fabrication and characterization of sodium cellulose sulfate -gelatin fibrous scaffolds with the goal of determining construct(s) that best support cell growth and infiltration. Aim 2 will determine sodium cellulose sulfate constructs that promote mesenchymal stem cell-induced cartilage formation and integration with surrounding host cartilage in vitro. The goals of this project are to develop a novel combination therapy for the repair of cartilage defects and enhance the scientific understanding of the role of biologically inspired materials and their effect on cell differentiation. The research efforts here will also be disseminated in teaching, training, and education. Of particular interest is the inclusion of underrepresented minorities and women in the scope of its impact. The principal investigator's laboratory is actively involved in the recruitment, training and mentorship of underrepresented minority and female students starting at the junior high school level through various community and university programs.This project will develop a biologically inspired material for cartilage tissue repair. Joint repair continues to be a significant challenge that demands the use of innovative materials that can provide biological function. Specialized constructs incorporating cells as well as materials that more closely mimic the structure of cartilage tissue during early development may be a promising strategy for promoting the formation of functional cartilage tissue. Sodium cellulose sulfate mimics the structure of glycosaminoglycans present in cartilage tissue and can be prepared with varying degrees of functional groups to improve its biological performance. Sodium cellulose sulfate containing materials will be evaluated with stem cells for the formation of cartilage tissue. The goals of this project are to develop a novel combination therapy for the repair of cartilage defects and enhance the scientific understanding of the role of biologically inspired materials and their effect on cell function. The research efforts here will also be disseminated in teaching, training, and education. Of particular interest is the inclusion of underrepresented minorities and women in the scope of its impact. The principal investigator's laboratory is actively involved in the recruitment, training and mentorship of underrepresented minority and female students starting at the junior high school level through various community and university programs.

该奖项由新泽西理工学院材料研究部生物材料项目颁发，将研究一种新型半合成纤维素衍生物，纤维素是最丰富的天然材料之一，可用作软骨修复的组织工程支架。关节软骨的愈合能力有限。在早期发育过程中，结合细胞和材料的特殊结构更接近地模拟软骨细胞外基质的结构，可能是促进功能性软骨组织形成的有前途的策略。纤维素硫酸钠是水溶性的，模仿糖胺聚糖的结构。它是一种线性多糖，可以合成不同程度的硫酸化以提高生物活性。 纤维素硫酸钠可以赋予与糖胺聚糖相似的功能品质，直接软骨形成和软骨组织形成。纤维素硫酸钠将与纤维形式的明胶结合，在结构和功能上更接近地模仿软骨的天然细胞外基质。该项目的首要目标是纤维素硫酸钠-明胶纤维支架的制造和表征，目的是确定最能支持细胞生长和浸润的构建体。目标 2 将确定在体外促进间充质干细胞诱导的软骨形成以及与周围宿主软骨整合的硫酸纤维素钠构建体。该项目的目标是开发一种用于修复软骨缺陷的新型联合疗法，并增强对生物启发材料的作用及其对细胞分化的影响的科学理解。这里的研究成果也将传播到教学、培训和教育中。 特别令人感兴趣的是，将代表性不足的少数群体和妇女纳入其影响范围。 首席研究员的实验室通过各种社区和大学项目，积极参与从初中阶段开始招募、培训和指导代表性不足的少数族裔和女学生。该项目将开发一种用于软骨组织修复的生物启发材料。关节修复仍然是一项重大挑战，需要使用能够提供生物功能的创新材料。包含细胞和材料的专门结构在早期发育过程中更接近地模拟软骨组织的结构可能是促进功能性软骨组织形成的有前途的策略。纤维素硫酸钠模仿软骨组织中存在的糖胺聚糖的结构，并且可以制备成具有不同程度的官能团以改善其生物性能。将使用干细胞评估含有纤维素硫酸钠的材料以形成软骨组织。该项目的目标是开发一种用于修复软骨缺陷的新型联合疗法，并增强对生物启发材料的作用及其对细胞功能影响的科学理解。这里的研究成果也将传播到教学、培训和教育中。 特别令人感兴趣的是，将代表性不足的少数群体和妇女纳入其影响范围。 首席研究员实验室通过各种社区和大学项目，积极参与从初中阶段开始招募、培训和指导代表性不足的少数族裔和女学生。