Studies of translation initiation in bacteria
细菌翻译起始的研究
基本信息
- 批准号:1614990
- 负责人:
- 金额:$ 64.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2016
- 资助国家:美国
- 起止时间:2016-07-15 至 2020-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This project will reveal the distinct way in which proteins are made in the Bacteroidetes, a family of bacteria that are found in the healthy human intestine as well as in many natural habitats. Bacteroidetes lacks the Shine-Dalgarno sequence, a well-known control elelment that is required for translation of RNA into proteins in most other bacteria, and this research is aimed at determining what takes its place in this bacterial lineage. This project will also provide (1) research training for undergraduate, graduate, and postdoctoral students, (2) summer research opportunities for local high school students (Columbus Public Schools), and (3) faculty mentorship for students of the Louis Stokes Alliance for Minority Participation (LSAMP) program, which aims to boost the success rate of underrepresented minority students in STEM (Science, Technology, Engineering, and Mathematics) disciplines.Initiation of translation involves assembly of a ribosome complex in which the initiator tRNA is paired to the start codon of mRNA. The fidelity of translation depends on recognition of the correct start codon among all AUG and similar trinucleotide sequences in the mRNA. In E. coli and many bacteria, recognition of the start codon is facilitated in part by base pairing between the Shine-Dalgarno (SD) sequence of the mRNA (which lies just upstream from the start codon), and the anti-Shine-Dalgarno (ASD) sequence of the 30S subunit (contained near the 3? end of the 16S rRNA). Recent genome sequencing studies have revealed that whole taxonomic groups of bacteria completely lack SD sequences, despite that the ASD sequence remains intact and conserved in these organisms. These include the phyla Bacteroidetes and a subset of Cyanobacteria. The primary goals of this project are to uncover the sequence determinants of the translation initiation regions (TIRs) in the Bacteroidetes and understand how these TIR sequences govern the rate of initiation. Modern techniques such as ribosome profiling will be used in conjunction with biochemical approaches to achieve these goals. Features of the TIR aside from the SD, such as secondary structure and S1 binding sites, are also thought to impact initiation rate in bacteria. By studying the Bacteriodetes system, the roles of these features may be clarified, as the complication of SD-ASD pairing is effectively removed.
这个项目将揭示类杆菌中蛋白质制造的独特方式,类杆菌是在健康的人类肠道和许多自然栖息地中发现的一类细菌。拟杆菌缺乏Shine-Dalgarno序列,这是一种众所周知的控制元件,在大多数其他细菌中是将RNA翻译成蛋白质所必需的,这项研究的目的是确定它在这一细菌谱系中的位置。该项目还将为本科生、研究生和博士后学生提供(1)研究培训,(2)为当地高中生(哥伦布公立学校)提供暑期研究机会,以及(3)为路易斯·斯托克斯少数民族参与联盟(LSAMP)计划的学生提供教师指导,该计划旨在提高STEM(科学、技术、工程和数学)学科中未被充分代表的少数族裔学生的成功率。翻译的启动涉及组装核糖体复合体,其中启动子tRNA与信使核糖核酸的起始密码子配对。翻译的保真度取决于在所有AUG和类似的三核苷酸序列中识别正确的起始密码子。在大肠杆菌和许多细菌中,起始密码子的识别部分是通过mRNA的Shine-Dalgarno(SD)序列(位于起始密码子的上游)与30S亚基的反Shine-Dalgarno(ASD)序列(包含在3?16S rRNA的末端)。最近的基因组测序研究表明,尽管ASD序列在这些生物中保持完整和保守,但整个细菌分类群完全缺乏SD序列。这些细菌包括拟杆菌门和蓝藻的一个亚群。该项目的主要目标是揭示拟杆菌中翻译起始区(TIR)的序列决定因素,并了解这些TIR序列如何控制起始率。核糖体图谱等现代技术将与生化方法结合使用,以实现这些目标。除了SD外,TIR的特征,如二级结构和S1结合位点,也被认为影响细菌的起始率。通过研究细菌系统,这些特征的作用可能被阐明,因为SD-ASD配对的复杂性被有效地消除了。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Kurt Fredrick其他文献
Errors rectified in retrospect
回顾时纠正的错误
- DOI:
10.1038/457157a - 发表时间:
2009-01-07 - 期刊:
- 影响因子:48.500
- 作者:
Kurt Fredrick;Michael Ibba - 通讯作者:
Michael Ibba
Protein L38 facilitates L6 incorporation during 50S subunit assembly in <em>Flavobacterium johnsoniae</em>
- DOI:
10.1016/j.bpj.2023.11.2209 - 发表时间:
2024-02-08 - 期刊:
- 影响因子:
- 作者:
Md Siddik Alom;Dominic Arpin;Brenna Hay;Zakkary McNutt;Leonard Foster;Joaquin Ortega;Kurt Fredrick - 通讯作者:
Kurt Fredrick
Kurt Fredrick的其他文献
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{{ truncateString('Kurt Fredrick', 18)}}的其他基金
Studies of protein synthesis in the Bacteroidia
拟杆菌中蛋白质合成的研究
- 批准号:
2344534 - 财政年份:2024
- 资助金额:
$ 64.5万 - 项目类别:
Standard Grant
Studies of protein synthesis in the Bacteroidetes
拟杆菌门蛋白质合成的研究
- 批准号:
2029502 - 财政年份:2020
- 资助金额:
$ 64.5万 - 项目类别:
Standard Grant
Studies of Translation Initiation in Bacteria
细菌翻译起始的研究
- 批准号:
1243997 - 财政年份:2013
- 资助金额:
$ 64.5万 - 项目类别:
Standard Grant
Studies of Translation Initiation in Bacteria
细菌翻译起始的研究
- 批准号:
0840996 - 财政年份:2009
- 资助金额:
$ 64.5万 - 项目类别:
Continuing Grant
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Studies of Translation Initiation in Bacteria
细菌翻译起始的研究
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