Role of RHAMNOSE BIOSYNTHESIS 1 in cell growth and patterning

鼠李糖生物合成 1 在细胞生长和模式形成中的作用

• 批准号: 1615387
• 负责人: Vivian Irish
• 金额: $ 78万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-15 至 2021-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1615387&HistoricalAwards=false
• 关键词: Role; RHAMNOSE; BIOSYNTHESIS; cell; growth

The study of surface patterning of plant cells is critical to understanding their function. These range from helping pollinating insects grip petals to decreasing the wettability of plant cell surfaces. In addition, understanding how this cell architecture is achieved can provide new approaches in nanotechnology and the design of novel bioinspired materials. This project combines genetics, biochemistry, and mathematical approaches to investigate how cell wall associated pectin molecules affect plant cell shape and surface pattern and contribute to the mechanical properties of plant cells. Through this project, undergraduates from underrepresented backgrounds, a graduate student and a postdoctoral associate will receive interdisciplinary training at the interface of experimental biology and mathematical modeling. In addition, the results from this project will be communicated to the public through outreach programs aimed at high school students and at the New Haven area community. Recent work from the PI's lab has shown that mutations in the gene encoding Rhamnose Biosynthesis 1 (RHM1) affect pectin composition and show striking patterning defects in cell morphology, resulting in cells with a left-handed helical twist and a reduction in anisotropic growth. While pectins have been shown to play important roles in regulating cell expansion, this is the first described helical mutant that affects pectin biosynthesis, and so provides an exciting new inroad into elucidating the potential roles of rhamnose-containing pectins in modulating plant cell shape. Through a combination of genetic and biochemical approaches with micro-mechanical testing and mathematical modeling, the role of rhamnose in controlling cell architecture will be elucidated. The results obtained through this project will have the potential to shed new light on how pectin composition can affect cell wall patterning and growth, providing an exciting link between enzyme activity, cell wall architecture, and supracellular patterning events. The combination of experimental approaches with testable mathematical models will lay the groundwork for understanding how the pectin gel matrix can feed back on cellular and subcellular processes necessary to regulate cell growth and form.

植物细胞表面图案的研究对于理解其功能至关重要。这些范围从帮助授粉昆虫抓住花瓣到降低植物细胞表面的润湿性。此外，了解这种细胞结构是如何实现的可以提供纳米技术和新型仿生材料设计的新方法。该项目结合了遗传学、生物化学和数学方法，研究细胞壁相关果胶分子如何影响植物细胞形状和表面图案，以及如何影响植物细胞的机械特性。通过这个项目，来自弱势群体的本科生、研究生和博士后将接受实验生物学和数学建模的跨学科培训。此外，该项目的结果将通过针对高中生和纽黑文地区社区的外展计划向公众传达。 PI 实验室最近的工作表明，编码鼠李糖生物合成 1 (RHM1) 的基因突变会影响果胶成分，并在细胞形态上显示出显着的图案缺陷，导致细胞呈左旋螺旋状，并减少各向异性生长。虽然果胶已被证明在调节细胞扩张中发挥重要作用，但这是第一个被描述的影响果胶生物合成的螺旋突变体，因此为阐明含鼠李糖果胶在调节植物细胞形状中的潜在作用提供了令人兴奋的新进展。通过将遗传和生化方法与微机械测试和数学模型相结合，鼠李糖在控制细胞结构中的作用将得到阐明。通过该项目获得的结果将有可能为果胶成分如何影响细胞壁图案和生长提供新的线索，提供酶活性、细胞壁结构和细胞上图案事件之间令人兴奋的联系。实验方法与可测试的数学模型的结合将为理解果胶凝胶基质如何反馈调节细胞生长和形成所需的细胞和亚细胞过程奠定基础。