SBIR Phase I: Genetic improvement of loblolly pine wood for increased density

SBIR 第一阶段：火炬松木材的遗传改良以增加密度

• 批准号: 1621939
• 负责人: Michael Harrington
• 金额: $ 22.5万
• 依托单位: Genoverde Biosciences, Incorporated
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1621939&HistoricalAwards=false
• 关键词: SBIR; Phase; Genetic; improvement; loblolly

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) project will be to increase wood production of managed loblolly pine tree farms by 20%. Global demand for forest industry products, e.g., pulp for paper, saw timber, and wood pellets for fuel, is expected to rise 60-100% by 2060. As the world's 4th largest exporter of forest products, the U.S. is well positioned to help meet these growing needs. To do so, managed loblolly pine tree farms will play a large role in supplying the demand. Using a biotechnology gene trait approach to introduce cell wall gene technology, the goal is to engineer pine trees with 20% more wood density over conventional crops. This may result in more wood material per tree, per acre with no added cost to production processing, i.e., no increase use of land, water, or fertilizers. As an added benefit, bioengineered trees with cell wall gene technologies aid in protecting the environment by sequestering more atmospheric CO2 thus helping to combat the negative effects of global climate change. Ultimately, this work may lead to the development of renewable materials needed to meet societal needs while helping to protect the environment. This Small Business Innovation Research Phase (SBIR) I project proposes to evaluate the use of a biotechnology gene trait approach to bioengineer loblolly pine for high wood density by modifying secondary cell wall gene regulation. The proposed research would utilize a newly adapted pine transformation protocol to introduce cell wall gene platform technologies, together with ubiquitously expressed and secondary wall-specific promoters, to significantly and selectively increase cell wall density in loblolly pine. Bioengineered plants will be selected using herbicide resistance and the effects of transgene incorporation analyzed through gene expression and histochemical analysis. The anticipated outcome of this project will be 400 bioengineered pine seedlings with greater strength and increased value to be tested in greenhouse and field trials once approved. This project will serve as proof-of-concept testing for select cell wall gene technologies in commercial tree crops towards technology commercialization.

这个小企业创新研究（SBIR）项目的更广泛的影响/商业潜力将是将火炬松管理农场的木材产量提高20%。全球对森林工业产品的需求，到2060年，用于造纸的纸浆、锯材和用于燃料的木屑颗粒预计将增长60-100%。作为世界第四大林产品出口国，美国有能力帮助满足这些日益增长的需求。要做到这一点，有管理的火炬松农场将在满足需求方面发挥重要作用。利用生物技术基因性状方法引入细胞壁基因技术，目标是设计出比传统作物木材密度高20%的松树。这可以导致每棵树、每英亩更多的木材，而不增加生产加工的成本，即，不增加土地、水和肥料的使用。作为一个额外的好处，具有细胞壁基因技术的生物工程树木有助于通过隔离更多的大气二氧化碳来保护环境，从而有助于应对全球气候变化的负面影响。最终，这项工作可能会导致可再生材料的开发，以满足社会需求，同时有助于保护环境。 这个小企业创新研究阶段（SBIR）I项目建议通过修改次生细胞壁基因调控来评估生物工程火炬松高木材密度的生物技术基因性状方法的使用。拟议的研究将利用一种新的适应性松树转化方案，引入细胞壁基因平台技术，以及普遍表达和次生壁特异性启动子，以显着和选择性地增加火炬松的细胞壁密度。将使用除草剂抗性选择生物工程植物，并通过基因表达和组织化学分析分析转基因掺入的影响。该项目的预期成果将是400株具有更大强度和更高价值的生物工程松树幼苗，一旦获得批准，将在温室和田间试验中进行测试。该项目将作为商业树木作物中选择细胞壁基因技术的概念验证测试，以实现技术商业化。