MRI:Acquisition of a Zeiss LSM 880 confocal microscope with Airyscan for research and training at North Carolina State University.

MRI：购买配备 Airyscan 的 Zeiss LSM 880 共焦显微镜，用于北卡罗来纳州立大学的研究和培训。

• 批准号: 1624613
• 负责人: Marcela Pierce
• 金额: $ 53.91万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1624613&HistoricalAwards=false
• 关键词: MRI; Acquisition; Zeiss; LSM; 880

An award is made to North Carolina State University (NCSU) to acquire a state-of the art confocal microscope in order train a well-prepared and knowledgeable workforce, as well as to conduct world-class research. This instrument will be used to train undergraduates, graduate students and postdocs in quantitative fluorescence microscopy applications and single molecule counting. The microscope will be located within the Cellular and Molecular Imaging Facility (CMIF), a University-administered core research facility that serves researchers from six NCSU colleges and from neighboring institutions and local industries. This system will expose students to state-of-the-art imaging applications via a new course (Quantitative Imaging and Dynamics in Biology), other established courses (BIT595 Confocal Microscopy and BIT 478/578 Mapping the Brain), and during direct undergraduate research experiences. In addition, a new and innovative Light Microscopy Workshop will serve under-represented students from local Historically Black Colleges and Universities (HBCUs) and a local women's college in North Carolina. By exposing traditionally underrepresented students to cutting edge research and technology, NCSU intends to broaden interest in and to improve STEM (Science, Technology, Engineering, Mathematics) education. This innovative program will i) expose students across the region to advanced imaging technologies that are not available at their home institutions; ii) introduce students at institutions lacking comprehensive research programs to ongoing research at NCSU; and iii) integrate and strengthen existing collaborations between NCSU and partnering institutions. NCSU will partner with the NC Museum of Natural Sciences to share imaging results via presentations and hands-on educational activities led by Workshop participants as well as students and postdocs from NCSU laboratories.The state of the confocal microscope will impact research and training programs across six NCSU colleges and numerous disciplines, including Cell and Molecular Biology, Biochemistry, Animal and Plant Developmental Biology, Plant Pathology, and Bio-Engineering, among others. This instrument will enable NCSU faculty and students to i) perform measurements with increased sensitivity and discrimination; ii) obtain fast temporal and spatial data; and iii) image live biological samples for longer times with reduced phototoxicity. These capabilities will impact, among others, research programs in plant vacuole biogenesis and dynamics, stem cell maintenance in plants, long-range signaling in the Drosophila embryo, and cell signaling during animal cell migration.

北卡罗莱纳州立大学（NCSU）获得了一项奖项，以获得最先进的共聚焦显微镜，以培养一支准备充分、知识渊博的员工队伍，并进行世界级的研究。该仪器将用于培养本科生，研究生和博士后定量荧光显微镜应用和单分子计数。该显微镜将位于细胞和分子成像设施（CMIF）内，这是一个由大学管理的核心研究设施，为来自NCSU六所学院以及邻近机构和当地工业的研究人员提供服务。该系统将通过新课程（定量成像和生物学动力学）、其他已建立的课程（BIT595共聚焦显微镜和bit478 /578大脑测绘）以及直接的本科研究经历，让学生接触到最先进的成像应用。此外，一个新的创新光学显微镜研讨会将为来自当地传统黑人学院和大学（HBCUs）和北卡罗莱纳州当地女子学院的代表性不足的学生提供服务。通过让传统上代表性不足的学生接触到前沿研究和技术，NCSU打算扩大对STEM（科学、技术、工程、数学）教育的兴趣并改善STEM教育。这一创新项目将i)让该地区的学生接触到本国学校所没有的先进成像技术；ii)向缺乏综合研究项目的院校的学生介绍我校正在进行的研究；整合和加强NCSU与伙伴机构之间的现有合作。NCSU将与NCSU自然科学博物馆合作，通过研讨会参与者以及NCSU实验室的学生和博士后领导的演示和实践教育活动来分享成像结果。共聚焦显微镜的现状将影响NCSU六所学院和众多学科的研究和培训计划，包括细胞和分子生物学、生物化学、动植物发育生物学、植物病理学和生物工程等。该仪器将使NCSU的教师和学生能够i)以更高的灵敏度和分辨力进行测量；Ii)获取快速的时空数据；iii)在降低光毒性的情况下，延长活体生物样品的成像时间。这些能力将影响植物液泡生物发生和动力学、植物干细胞维持、果蝇胚胎的远程信号传导以及动物细胞迁移过程中的细胞信号传导等研究项目。