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Molecular principles and control of cellular stress responses upon temperature and oxygen stress in Daphnia

水蚤温度和氧应激下细胞应激反应的分子原理和控制

基本信息

批准号：
225900048
负责人：
Professor Dr. Rüdiger J. Paul
金额：
--
依托单位：
Institut für Integrative Zellbiologie und Physiologie (IIZP)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2012
资助国家：
德国
起止时间：
2011-12-31 至 2015-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/225900048?language=en
关键词：
Molecular principles control cellular stress

项目摘要

Knowledge of causal connections between severe environmental changes (stressors), stress signaling, and stress-induced gene expressions is particularly important for an understanding of environmental acclimatization and evolutionary adaptation. Consequently, this application focuses on the nature of stress signaling in conjunction with stress-induced gene expressions in Daphnia. The connections between stressors, stress signals, transcription factor levels, and cellular stress responses (gene expressions on transcript and protein levels) will be investigated upon temperature and oxygen stress in a time-resolved manner in order to analyze and understand the molecular principles and control of cellular stress responses in Daphnia. As experimental animals, two Daphnia species (D. pulex and D. magna) will be used, which offer exceptional experimental advantages as genetic (http://daphnia.cgb.indiana.edu), biochemical or physiological model organisms, and for which differences in stress response dynamics have been proven. As add-on projects, effects of the stressor ‚starvation‘ on stress signals and responses will be studied with the main focus on iron metabolism and homeostasis, and the relationship between de novo glutathione and protein syntheses will be investigated to test for possible negative effects of elevated protein synthesis rate on antioxidant buffering capacity.

严重的环境变化（压力），压力信号和压力诱导的基因表达之间的因果关系的知识是特别重要的环境适应和进化适应的理解。因此，本申请的重点是压力信号的性质与压力诱导的基因表达水蚤。应激源，应激信号，转录因子水平和细胞应激反应（转录本和蛋白质水平上的基因表达）之间的联系将在温度和氧胁迫下以时间分辨的方式进行研究，以分析和理解水蚤细胞应激反应的分子原理和控制。作为实验动物，两种水蚤（D. pulex和D. magna），其作为遗传（http：//www.example.com）、生物化学或生理学模式生物提供了特殊的实验优势，并且已经证明了其在应激反应动力学方面的差异。daphnia.cgb.indiana.edu作为附加项目，将研究应激源“饥饿”对应激信号和反应的影响，主要关注铁代谢和体内平衡，并将研究从头谷胱甘肽和蛋白质合成之间的关系，以测试蛋白质合成速率升高对抗氧化缓冲能力可能产生的负面影响。