EAGER: Non-invasive Sensing of Superficial Organ Tissue via Conforming Multi-parametric Microfluidic Organ Biosensors (MMOBs): Shifting the Paradigm for Organ Assessment

PI: Johnson， Blake提案号：1650601用于移植的器官，如肾脏，供不应求。目前还没有预测移植目标器官健康状况的良好措施。目前的做法并不客观。该项目将使用当前最先进的方法来制造设备，这些设备将连接到一个特殊的设备中，用于使用传感器获得重要的测量结果，这些传感器将作为项目的一部分制造。目标是这样的测量将消除器官移植过程中健康评估的猜测工作。该项目的目标是制造一种符合要求且灵活的传感器，该传感器将适应离体器官表面（实验中的猪肾），用于获取电阻抗数据，并使用微流控装置测量灌注液体中的生物标志物。该项目将使用3D结构光扫描和基于挤压的增材制造方法的先进制造方法来制造保形（合形）微流体器官生物传感器。这种基于传感器的浅表组织基质中生物标志物和阻抗特征的识别有望与器官健康相关。该项目将举办为期两周的讲习班，培训学生制造生物医学设备，从而提供教育机会。教育效益将通过教师评议的BIO-Maker博览会进行评估。

PI: Johnson, Blake Proposal Number: 1650601 Organs for transplantation, such as kidneys, are in short supply. Good measures that predict the health status of organs targeted for transplantation are not available. The current approach is not objective. This project will use current state of the art methods to make devices that will be attached to live kidneys in a special apparatus for obtaining important measurements using sensors that will be fabricated as part of the project. The goal is that such measurements will take the guess work out of the health assessment of the organ transplantation procedure. The goal of the proposed project is to fabricate a conforming and flexible sensor that will be adaptive to ex vivo organ surfaces (the porcine kidney in the experiments) for obtaining electrical impedance data, and measure biomarkers in perfused fluids using a microfluidic arrangement. The project will use advanced fabrication approach of 3D structured light scanning and extrusion-based additive manufacturing methodology for fabricating conformal (form-fitting) microfluidic organ biosensors. This sensor-based identification of biomarkers and impedance signatures in superficial tissue matrix is expected to correlate with organ health. The project will provide an educational opportunity by creating a two-week workshop which will train students in biomedical device manufacturing. Educational benefits will be assessed through a faculty-judged BIO-Maker fair.