Collaborative Research: Microbicidal Carbon Dots for Combating Anti-Biotic Resistance and Beyond

合作研究：用于对抗抗生素耐药性等的杀菌碳点

• 批准号: 1701424
• 负责人: Ya-Ping Sun
• 金额: $ 18万
• 依托单位: Clemson University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1701424&HistoricalAwards=false
• 关键词: Collaborative; Research; Microbicidal; Carbon; Dots

Non-Technical Abstract: Since the 1990s, infectious diseases caused by antibiotic-resistant bacteria have been increasing globally and becoming a serious threat from these diseases. Antibiotic resistance is mostly a consequence of bacterial response to excessive uses of antibiotics in humans or agriculture and the widespread usage of disinfectants in a variety of other settings. Noticeably, new resistance mechanisms emerge and spread globally as fast as new drugs being developed, which has posted serious challenges to the realm of traditional antibiotics/antimicrobial agents, but has also motivated a global search for alternative antimicrobial strategies. Among the many strategies that have been pursued in combating the resistance, the development of antibacterial agents that are mechanistically different from traditional antibiotics is a more effective option. Specifically, on the technology explored in this project, carbon dots are developed as a new class of antimicrobial agents which kill bacterial cells through reactive species generated under visible/natural light. This project, funded by the Biomaterials Program within the National Science Foundation's Division of Materials Research, focuses on the evaluation and validation of the potential of carbon dots for killing drug-resistant bacteria and developing an understanding of related working mechanisms, with the goal of establishing an effective alternative antimicrobial technology for combating drug-resistant bacteria. This project will also have significant broader impacts in stimulating the development and transfer of new technologies for the knowledge-driven economy, and other areas such as applications in food, water safety and national defense, education and human resource development. With more than 85% of the student population from underrepresented minority groups, the proposed multidisciplinary research is expected in providing a great research training environment for these undergraduate students in a variety of ways, including direct participation in research, student exchange programs (between the collaborators' labs), and summer workshops. Technical Abstract: This Collaborative Research project is to explore the light-activated microbicidal functions of newly developed carbon dots (CDots) against drug-resistant bacterial pathogens. CDots may be considered as a special kind of "core-shell" nanostructures, each with a small carbon nanoparticle core and a thin shell of soft materials. They are strongly absorptive over the entire visible spectrum, and their photoexcited state properties and redox processes resemble those typically found in semiconductor quantum dots, but with unique advantages. The interdisciplinary project team will develop and validate CDots as a new class of visible/natural light-activated microbicidal agents against multi-drug resistant bacteria, with the selected species for testing including Salmonella enterica, Listeria monocytogenes, and Campylobacter jejuni and Campylobacter coli isolated from farm animals. The scientific objectives of the project are: (1) systematic evaluation of CDots' antibacterial function to the drug-resistant bacteria in relation to several major factors including Cdot surface charge, size, and synthesis approach; (2) mechanistic elucidation and understanding of CDots' antimicrobial function, focusing on correlations of the structural and photochemical parameters of CDots with the observed bacteria-killing performances; and (3) exploration of Carbon/TiO2 hybrid dots of different configurations for enhanced antimicrobial performance against drug-resistant microbes and viruses. The intellectual merits of the project include the advancement of the biomaterial field for antimicrobial applications and the establishment of the photo-activated antimicrobial functions of CDots for combating the increasing threat of antibiotic resistance. In addition to addressing the critical issues in national healthcare, these materials could find applications in food, water safety and national defense. The broad impacts of the project include providing excellent research-for-training opportunities for participating students in the interdisciplinary field at the interface of material science and biological applications at the two collaborating institutions, especially for undergraduate and K-12 students from underrepresented groups.

非技术摘要：自20世纪90年代以来，全球范围内由抗生素耐药细菌引起的传染病不断增加，并成为这些疾病的严重威胁。抗生素耐药性主要是细菌对人类或农业中过度使用抗生素以及在各种其他环境中广泛使用消毒剂产生反应的结果。值得注意的是，新的耐药机制的出现和全球传播的速度与新药的开发速度一样快，这对传统抗生素/抗菌药物领域提出了严峻的挑战，但也促使全球寻找替代的抗菌策略。在对抗耐药性的众多策略中，开发与传统抗生素在机制上不同的抗菌剂是更有效的选择。具体来说，在该项目探索的技术中，碳点被开发为一类新型抗菌剂，它通过可见光/自然光下产生的活性物质杀死细菌细胞。该项目由美国国家科学基金会材料研究部生物材料计划资助，重点评估和验证碳点杀死耐药细菌的潜力，并了解相关工作机制，目标是建立一种有效的替代抗菌技术来对抗耐药细菌。该项目还将在刺激知识驱动型经济的新技术开发和转让以及食品、水安全和国防、教育和人力资源开发等其他领域的应用方面产生更广泛的影响。由于超过 85% 的学生来自代表性不足的少数群体，拟议的多学科研究预计将以多种方式为这些本科生提供良好的研究培训环境，包括直接参与研究、学生交流计划（合作者实验室之间）和夏季研讨会。技术摘要：该合作研究项目旨在探索新开发的碳点（CDots）对耐药细菌病原体的光激活杀菌功能。 CDots可以被认为是一种特殊的“核-壳”纳米结构，每个结构都有一个小的碳纳米颗粒核和一个软材料薄壳。它们在整个可见光谱范围内具有很强的吸收性，其光激发态特性和氧化还原过程类似于半导体量子点中常见的那些，但具有独特的优势。跨学科项目团队将开发和验证 CDots 作为一种新型可见光/自然光激活的杀菌剂，对抗多重耐药细菌，测试的选定物种包括肠沙门氏菌、单核细胞增生李斯特氏菌、从农场动物中分离出的空肠弯曲杆菌和大肠杆菌。该项目的科学目标是：（1）系统评价CDots对耐药菌的抗菌功能，与Cdot表面电荷、尺寸和合成方法等几个主要因素相关； （2）对CDots抗菌功能的机理阐明和理解，重点关注CDots的结构和光化学参数与观察到的杀菌性能的相关性； (3)探索不同结构的碳/TiO2杂化点以增强对耐药微生物和病毒的抗菌性能。该项目的智力优势包括抗菌应用生物材料领域的进步以及建立CDots的光激活抗菌功能以应对日益严重的抗生素耐药性威胁。除了解决国家医疗保健中的关键问题外，这些材料还可以在食品、水安全和国防方面得到应用。该项目的广泛影响包括为两个合作机构的材料科学和生物应用交叉学科跨学科领域的参与学生提供极好的研究培训机会，特别是为来自代表性不足群体的本科生和 K-12 学生。

项目成果

Synergistic photoactivated antimicrobial effects of carbon dots combined with dye photosensitizers

• DOI: 10.2147/ijn.s183086
• 发表时间: 2018-01-01
• 期刊: INTERNATIONAL JOURNAL OF NANOMEDICINE
• 影响因子: 8
• 作者: Dong, Xiuli;Bond, Ambrose E.;Yang, Liju
• 通讯作者: Yang, Liju

Carbon Dots Incorporated Multi-walled Carbon Nanotube Coated Filters for Bacterial Removal and Inactivation.

碳点采用多壁碳纳米管涂层过滤器，用于去除和灭活细菌。

• 发表时间: 2018
• 期刊: RSC advances
• 影响因子: 3.9
• 作者: Xiuli Dong1, Mohamad Al

Chemical Reactions in Thermal Carbonization Processing of Citric Acid-Urea Mixtures

• DOI: 10.21127/yaoyigc20210011
• 发表时间: 2021
• 期刊: General Chemistry
• 作者: Weixiong Liang;Ping Wang;Liju Yang;Christopher M. Overton;B. Hewitt;Ya‐Ping Sun

Photoexcited state properties and antibacterial activities of carbon dots relevant to mechanistic features and implications

• DOI: 10.1016/j.carbon.2020.08.025
• 发表时间: 2020-12-01
• 期刊: CARBON
• 影响因子: 10.9
• 作者: Dong, Xiuli;Ge, Lin;Sun, Ya-Ping
• 通讯作者: Sun, Ya-Ping

Optical and photodynamic properties of carbon/TiO2 hybrid dots in different nanoscale configurations

• DOI: 10.1016/j.cplett.2020.137208
• 发表时间: 2020-03
• 期刊: Chemical Physics Letters
• 影响因子: 2.8
• 作者: Nengyu Pan;Peter A. Okonjo;Ping Wang;Yongan Tang;Ya‐Ping Sun;Liju Yang