DISSERTATION RESEARCH: Role of non-structural carbohydrate dynamics in legacy effects of drought in Southwestern forests

论文研究：非结构碳水化合物动态在西南森林干旱遗留影响中的作用

• 批准号: 1702017
• 负责人: Kiona Ogle
• 金额: $ 1.98万
• 依托单位: Northern Arizona University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-01 至 2019-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1702017&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Role; non; structural

Drought is becoming more common in the southwestern US, resulting in higher tree mortality. Surviving trees may experience legacy effects of drought: decreases in growth rates for multiple years. These trees may not fully recover before the next drought arrives, increasing the risk that more will die. One potential mechanism driving these legacy effects is related to how trees store and use sugars. Drought may change trees' ability to use stored sugars, because they are moved by water in tree tissues. This Doctoral Dissertation Improvement Grant (DDIG) will provide funds to compare trees under different levels of drought stress to understand how drought changes the availability of sugars in trees, and how these changes are related to tree growth rates and legacy effects, using tree rings and the age of sugars in different rings. This research is important because it will improve understanding of how trees respond physiologically to drought, which could improve predictions of how environmental change will affect forests. Accurate predictions about drought effects on US forests is critical for management of associated local (fire, flooding, etc.) and global (altered carbon cycling) negative impacts.This study will address: (1) what are the effects of drought stress on storage dynamics of non-structural carbohydrates (NSCs) in foundation tree species, and, (2) how are these dynamics related to legacy effects of drought in southwestern US forests? This project focuses on two foundational Populus species, because angiosperms exhibit complex drought legacy effects and are more reliant on NSC storage. Tree cores from 14 genotypes of Populus fremontii experiencing varying levels of apparent drought stress at the Palo Verde Common Garden were collected in 2015. Tree cores and respired CO2 will also be collected from Populus tremuloides individuals at four sites differing in drought stress across the Four Corners region. This study will analyze tree-ring wood and gas samples for 14C content to assess carbon fixation date, age, and availability of NSCs in tree rings of different ages, and for delta13C to assess drought stress. 14C and delta13C data will be related to ring widths and water stress/use indices using hierarchical models to understand the role of NSC dynamics in drought legacy effects. This study will develop an inexpensive, novel incubation method for obtaining CO2 for 14C analysis, bypassing existing difficulties with NSC extraction procedures. These isotopic methods will help elucidate links between drought stress and NSC storage, with implications for how foundation tree species will respond to climate change.

干旱在美国西南部变得越来越普遍，导致树木死亡率上升。幸存的树木可能会经历干旱的遗留影响：多年的生长率下降。这些树木在下一次干旱到来之前可能无法完全恢复，这增加了更多树木死亡的风险。驱动这些遗留效应的一个潜在机制与树木如何储存和使用糖有关。干旱可能会改变树木利用储存的糖的能力，因为它们是由树木组织中的水移动的。该博士论文改进补助金（DDIG）将提供资金，用于比较不同干旱胁迫水平下的树木，以了解干旱如何改变树木中糖的可用性，以及这些变化如何与树木的生长速度和遗产效应相关，使用树木年轮和不同年轮中糖的年龄。这项研究很重要，因为它将提高对树木对干旱的生理反应的理解，这可以改善对环境变化如何影响森林的预测。准确预测干旱对美国森林的影响对于管理相关的地方（火灾，洪水等）至关重要。这项研究将解决：（1）干旱胁迫对基础树种非结构性碳水化合物（NSC）储存动态的影响，以及（2）这些动态与美国西南部森林干旱的遗留影响有何关系？该项目的重点是两个基本的杨属物种，因为被子植物表现出复杂的干旱遗留效应，更依赖于NSC存储。2015年，在帕洛韦尔迪公共花园收集了14种经历不同程度明显干旱胁迫的费氏杨基因型的树芯。树芯和呼吸的二氧化碳也将收集从胡杨个人在四个站点不同的干旱胁迫在四个角落地区。这项研究将分析树木年轮木材和气体样品的14 C含量，以评估碳固定日期，年龄和不同年龄的树木年轮中神经干细胞的可用性，并为delta 13 C评估干旱胁迫。14 C和delta 13 C数据将与环宽度和水压力/使用指数使用层次模型，以了解干旱遗留效应的NSC动态的作用。这项研究将开发一种廉价的，新颖的培养方法，以获得CO2的14 C分析，绕过现有的困难与NSC提取程序。这些同位素方法将有助于阐明干旱胁迫和NSC储存之间的联系，并对基础树种如何应对气候变化产生影响。