Direct Observation of Vesicle Dynamics, Collision, and Adhesion

直接观察囊泡动力学、碰撞和粘附

• 批准号: 1704668
• 负责人: Charles Schroeder
• 金额: $ 30万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1704668&HistoricalAwards=false
• 关键词: Direct; Observation; Vesicle; Dynamics; Collision

CBET - 1704668PI: Schroeder, CharlesThis award will support an experimental and numerical study of the dynamics of vesicles, particles composed of liquid or gas that is surrounded by a membrane, suspended in flow. The vesicles in this project are liquid-filled and are surrounded by a lipid bilayer membrane, which makes them similar to vesicles found in biological systems. They are also similar to synthetic vesicles that are used in a variety of consumer products and foods. The goals of the project are to characterize dynamics of the vesicles in flow, to use the behavior of the vesicles to characterize their mechanical properties, and to study collisions between vesicles and their adhesion. To accomplish these goals, vesicles of known composition will be placed in a microfluidic cell that has multiple fluidic channels arranged to keep the vesicle stationary in the surrounding flow. The deformation of single vesicles and collisions among vesicles will be viewed through a microscope. The results of the project will enable practitioners to design robust and stable vesicles for use in products. The project team will participate in the Illinois iRise program, which works with teachers to develop hands-on experimental labs for K-12 and high school students. The team will also develop hands-on demonstrations for students from underrepresented groups participating in local Boys and Girls Clubs.The dynamics of single vesicles will be studied in precisely controlled steady and oscillatory extensional flow. Parameters that affect vesicle shapes and the transition from stable to unstable shapes will be examined, including flow rate, membrane bending energy, membrane elasticity and viscosity. A phase diagram for vesicle stability and deformation in extensional flow will be constructed. Results from the project will help resolve discrepancies between experiments and simulations regarding the onset of vesicle shape instabilities such as pearling. The oscillatory flow used in this project will expose vesicles to time-dependent strains, which will expose the effect of unsteadiness on shape instabilities. Collisions and adhesion of freely-suspended vesicles will be characterized. Most prior work has focused on the weak interaction limit, wherein the membrane bending energy plays a dominant role and membrane elasticity is not relevant. However, vesicle membranes deform and stretch upon collision, which has been predicted to give rise to enhanced interactions and unexpected aggregation. The experiments in this project will study collision and adhesion under these conditions.

CBET - 1704668PI：Schroeder，Charles 该奖项将支持对囊泡动力学的实验和数值研究，囊泡是由液体或气体组成的颗粒，被膜包围，悬浮在流动中。 该项目中的囊泡充满液体，并被脂质双层膜包围，这使得它们类似于生物系统中发现的囊泡。 它们也类似于用于各种消费品和食品的合成囊泡。 该项目的目标是表征囊泡的流动动力学，利用囊泡的行为来表征其机械特性，并研究囊泡之间的碰撞及其粘附。 为了实现这些目标，已知组成的囊泡将被放置在微流体单元中，该微流体单元具有多个排列成使囊泡在周围流动中保持静止的流体通道。 通过显微镜观察单个囊泡的变形和囊泡之间的碰撞。 该项目的结果将使从业者能够设计出用于产品的坚固且稳定的囊泡。 该项目团队将参与伊利诺伊州 iRise 计划，该计划与教师合作，为 K-12 和高中生开发动手实验实验室。 该团队还将为参加当地男孩和女孩俱乐部的代表性不足群体的学生开发动手演示。单个囊泡的动力学将在精确控制的稳定和振荡拉伸流中进行研究。 将检查影响囊泡形状以及从稳定形状到不稳定形状转变的参数，包括流速、膜弯曲能、膜弹性和粘度。 将构建囊泡稳定性和拉伸流动变形的相图。 该项目的结果将有助于解决有关囊泡形状不稳定（例如珍珠化）发生的实验和模拟之间的差异。该项目中使用的振荡流将使囊泡暴露于时间依赖性应变，这将暴露不稳定对形状不稳定性的影响。 将表征自由悬浮的囊泡的碰撞和粘附。 大多数先前的工作都集中在弱相互作用极限上，其中膜弯曲能起主导作用，膜弹性不相关。然而，囊泡膜在碰撞时会变形和拉伸，预计这会导致相互作用增强和意外的聚集。该项目的实验将研究这些条件下的碰撞和粘附。

项目成果

Double-mode relaxation of highly deformed anisotropic vesicles

高度变形各向异性囊泡的双模式弛豫

• DOI: 10.1103/physreve.102.010605
• 发表时间: 2020
• 期刊: Physical Review E
• 影响因子: 2.4
• 作者: Kumar, Dinesh;Richter, Channing M.;Schroeder, Charles M.
• 通讯作者: Schroeder, Charles M.

Orientation control and nonlinear trajectory tracking of colloidal particles using microfluidics

使用微流体的胶体颗粒的方向控制和非线性轨迹跟踪

• DOI: 10.1103/physrevfluids.4.114203
• 发表时间: 2019
• 期刊: Physical Review Fluids
• 影响因子: 2.7
• 作者: Kumar, Dinesh;Shenoy, Anish;Li, Songsong;Schroeder, Charles M.
• 通讯作者: Schroeder, Charles M.

Flow Topology During Multiplexed Particle Manipulation Using a Stokes Trap

使用斯托克斯陷阱进行多重粒子操纵期间的流拓扑

• DOI: 10.1103/physrevapplied.12.054010
• 发表时间: 2019
• 期刊: Physical Review Applied
• 影响因子: 4.6
• 作者: Shenoy, Anish;Kumar, Dinesh;Hilgenfeldt, Sascha;Schroeder, Charles M.
• 通讯作者: Schroeder, Charles M.

Automation and flow control for particle manipulation

• DOI: 10.1016/j.coche.2020.02.006
• 发表时间: 2020-09-01
• 期刊: CURRENT OPINION IN CHEMICAL ENGINEERING
• 影响因子: 6.6
• 作者: Kumar, Dinesh;Shenoy, Anish;Schroeder, Charles M.
• 通讯作者: Schroeder, Charles M.

Conformational dynamics and phase behavior of lipid vesicles in a precisely controlled extensional flow

精确控制的拉伸流中脂质囊泡的构象动力学和相行为

• DOI: 10.1039/c9sm02048a
• 发表时间: 2020
• 期刊: Soft Matter
• 影响因子: 3.4
• 作者: Kumar, Dinesh;Richter, Channing M.;Schroeder, Charles M.
• 通讯作者: Schroeder, Charles M.