Conformational Equilibria of Biologically Relevant Oligosaccharides

生物学相关寡糖的构象平衡

• 批准号: 1707660
• 负责人: Anthony Serianni
• 金额: $ 75万
• 依托单位: University of Notre Dame
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1707660&HistoricalAwards=false
• 关键词: Conformational; Equilibria; Biologically; Relevant; Oligosaccharides

With this award, the Chemistry of Life Processes Program in the Chemistry Division is funding Professor Anthony Serianni from the University of Notre Dame to investigate the three-dimensional shape of sugar molecules in solution. Beyond their nutritional value, sugars play numerous other critical roles in biology. For example, they are attached to the outside of cells like an identification number for cell-cell recognition. Recognition depends on the specific shapes the molecules adopt on the cell surface. They fit together much like the pieces of a puzzle. The shapes of deoxyribonucleic acid (DNA) and protein molecules are determined routinely, but sugar structures defy solution so far. The research targets the discovery of a general method for the determination of sugar structures in solution based on both experiments and theory. When the experimental structures match the theoretical ones, there is confidence that the shape is obtained correctly. This new approach is entirely general, applicable to any sugar in solution. Senior investigators, postdoctoral fellows, graduate students and undergraduates with interests and expertise in experimental and computational science collaborate to achieve the goals of the project. Research by Professor Serianni has been commercialized at Omicron Biochemicals, Inc., which he cofounded in 1982. Omicron prepares sugars and their derivatives based on novel reactions. These sugars are used in fundamental chemical, biochemical and biological research, in biomedical and clinical studies, and in the development of diagnostic tools. Omicron regularly collaborates with the Professor Serianni's laboratory at Notre Dame by providing synthetic expertise and reagents to support fundamental and applied studies of sugar chemistry and biochemistry.This research project uses stable isotopically labeled oligosaccharides, nuclear magnetic resonance (NMR) spin-couplings, and density functional theory calculations and circular statistics to determine conformational populations of the flexible domains of oligosaccharides in solution. This experimental approach is possible because saccharides contain multiple redundant NMR spin-couplings that report on the same conformational domain, allowing quantitative treatments that yield populational models. Depending on the domain, single- and multi-state models are tested. Once a given domain is parameterized (e.g., an O-glycosidic linkage), its conformational properties are investigated in different structural contexts to determine the degree to which it adapts conformationally to environmental cues. Experimentally-derived models are compared to those obtained from molecular dynamics simulations to validate the latter. This work may lead to new experiment-based conformational classifications of O-glycosidic linkages in oligosaccharides, and to a deeper understanding of the multiple factors that influence oligosaccharide conformation in solution.

有了这个奖项，化学系的生命过程化学项目资助圣母大学的Anthony Serianni教授研究溶液中糖分子的三维形状。 除了它们的营养价值，糖在生物学中还发挥着许多其他关键作用。 例如，它们附着在细胞的外部，就像一个识别号码，用于细胞间的识别。 识别取决于分子在细胞表面上采用的特定形状。它们就像拼图一样拼凑在一起。 脱氧核糖核酸（DNA）和蛋白质分子的形状是常规确定的，但糖的结构迄今为止还无法解决。 本研究的目标是在实验和理论的基础上发现一种测定溶液中糖结构的通用方法。 当实验结构与理论结构相匹配时，可以确信正确获得了形状。 这种新方法是完全通用的，适用于溶液中的任何糖。 高级研究人员，博士后研究员，研究生和本科生的兴趣和专业知识在实验和计算科学合作，以实现该项目的目标。 Serianni教授的研究已经在Omicron Biochemicals，Inc.商业化，他在1982年与人共同创立了这家公司。Omicron基于新颖的反应制备糖及其衍生物。 这些糖用于基础化学，生物化学和生物学研究，生物医学和临床研究以及诊断工具的开发。Omicron定期与圣母大学Serianni教授的实验室合作，提供合成专业知识和试剂，以支持糖化学和生物化学的基础和应用研究。该研究项目使用稳定的同位素标记寡糖，核磁共振（NMR）自旋耦合，和密度泛函理论计算和循环统计，以确定低聚糖的柔性结构域的构象群体，溶液 这种实验方法是可能的，因为糖包含多个冗余的核磁共振自旋偶联，可以报告相同的构象域，从而允许定量处理，产生种群模型。根据域，单状态和多状态模型进行测试。 一旦给定域被参数化（例如，一个O-糖苷键），其构象特性进行了研究，在不同的结构背景下，以确定其适应构象环境线索的程度。 实验得出的模型进行比较，从分子动力学模拟，以验证后者。这项工作可能会导致新的实验为基础的构象分类的O-糖苷键的寡糖，并更深入地了解的多种因素，影响寡糖的构象在溶液中。

项目成果

Conformational analysis of the disaccharide methyl α- D -mannopyranosyl-(1→3)-2- O -acetyl-β- D -mannopyranoside monohydrate

二糖甲基α-D-吡喃甘露糖基-(1-3)-2-O-乙酰基-β-D-吡喃甘露糖苷一水合物的构象分析

• DOI: 10.1107/s2053229619004728
• 发表时间: 2019
• 期刊: Acta Crystallographica Section C Structural Chemistry
• 作者: Zhang, Wenhui;Wu, Qingquan;Oliver, Allen G.;Serianni, Anthony S.
• 通讯作者: Serianni, Anthony S.

O -Benzoyl side-chain conformations in 2,3,4,6-tetra- O -benzoyl-β- D -galactopyranosyl-(1→4)-1,2,6-tri- O -benzoyl-β- D -glucopyranose (ethyl acetate solvate) and 1,2,4,6-tetra- O -benzoyl-β- D -glucopyranose (acetone solvate)

2,3,4,6-四- O -苯甲酰基-β- D -吡喃半乳糖基-(1â4)-1,2,6-三- O -苯甲酰基-β- D 中的 O -苯甲酰基侧链构象

• DOI: 10.1107/s2053229619000822
• 发表时间: 2019
• 期刊: Acta Crystallographica Section C Structural Chemistry
• 作者: Turney, Toby;Pan, Qingfeng;Zhang, Wenhui;Oliver, Allen G.;Serianni, Anthony S.
• 通讯作者: Serianni, Anthony S.