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Bioorigami Hydrogels composed of Natural and Synthetic Biomaterials

由天然和合成生物材料组成的生物折纸水凝胶

基本信息

批准号：
1709349
负责人：
David Gracias
金额：
$ 42万
依托单位：
Johns Hopkins University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-06-01 至 2021-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1709349&HistoricalAwards=false
关键词：
Bioorigami Hydrogels composed Natural Synthetic

项目摘要

Non-technical:A common geometric architecture for naturally occurring biomaterials is a hierarchical curvature and folding design that can be observed in a wide range of living systems such as the gyri in the brain or the microvilli on intestinal cells or the curved sporangia in fungi. Yet a fundamental understanding of the behavior of cells in relationship to this curvature and folding is lacking. This award seeks to self-assemble and characterize both synthetic and cell-derived composite hydrogels in biomimetic curved and folded geometries and characterize the behavior of the biomaterials and of the cells in these environments. Insights gained through this work may inform efforts to understand, support, and enhance development and recovery from injury in both the plant and the animal kingdoms. In addition to research, an emphasis will also be made on augmenting graduate education through curriculum changes, undergraduate and K-12 education through research experiences, as well as outreach to the local school system and enhancing excitement of biomaterials and tissue engineering to the broader public and society through lecture-demonstrations and joint projects with educators in the creative arts. Technical:This award supports the creation and characterization of model bio-origami hydrogels composed of synthetic and cell-derived biomaterials, elucidation of the mechanisms for elevated activity of biochemical and small molecule production by cells in these geometries and investigation of the role of layering and fluidic gradients in curved and folded geometries during development, healing, and homeostasis. In the project, model hydrogel systems will be created so that the roles of folding pitch, curvature, three-dimensionality, and strain on cellular behavior can be individually isolated and interrogated. The methods used in this investigation will include the following: micro- and nano-patterning, three-dimensional structuring, strain engineering, material characterization, biochemical and cellular assays including in vitro reporters of signaling pathway activation and cytoskeletal reorganization, quantitative 3D image analysis and optical slicing, lattice light sheet microscopy, statistical design of experiments, and finite element modeling. This research program will catalyze multidisciplinary collaborative endeavor integrating cell biology and engineering, and create avenues for unique combinatorial training for graduate students in techniques and study designs that challenge the current state of the art.

非技术性：自然产生的生物材料的常见几何结构是层次化的曲率和折叠设计，可以在广泛的生命系统中观察到，例如大脑中的回回或肠道细胞上的微绒毛，或真菌中弯曲的孢子囊。然而，对细胞与这种弯曲和折叠的关系的行为缺乏基本的理解。该奖项旨在以仿生、弯曲和折叠的几何形状自组装和表征合成和细胞衍生的复合水凝胶，并表征生物材料和细胞在这些环境中的行为。通过这项工作获得的洞察力可能有助于理解、支持和加强植物和动物王国中的发展和从伤害中恢复的努力。除研究外，还将强调通过课程改革加强研究生教育，通过研究经验加强本科和K-12教育，扩大与当地学校系统的联系，并通过讲座示范和与创造性艺术教育工作者的联合项目，增强生物材料和组织工程对更广泛的公众和社会的兴奋。技术：该奖项支持由合成生物材料和细胞衍生生物材料组成的生物折纸水凝胶模型的创建和表征，阐明这些几何形状的细胞提高生化和小分子生产活性的机制，以及研究弯曲和折叠几何形状的分层和流体梯度在发育、愈合和动态平衡过程中的作用。在该项目中，将创建模型水凝胶系统，以便可以单独隔离和询问折叠间距、曲率、三维和应变对细胞行为的作用。研究中使用的方法包括：微纳图案化、三维结构、应变工程、材料表征、生化和细胞分析(包括信号通路激活和细胞骨架重组的体外报告)、定量三维图像分析和光学切片、晶格光片显微镜、实验统计设计和有限元建模。这项研究计划将促进整合细胞生物学和工程学的多学科合作努力，并为研究生在挑战当前艺术水平的技术和研究设计方面的独特组合培训创造途径。