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EAGER: Origami inspired 3D Biosensors for Single Cell Analysis

EAGER：受折纸启发的 3D 生物传感器用于单细胞分析

基本信息

批准号：
1442014
负责人：
David Gracias
金额：
$ 15万
依托单位：
Johns Hopkins University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2014
资助国家：
美国
起止时间：
2014-08-01 至 2016-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1442014&HistoricalAwards=false
关键词：
EAGER Origami inspired 3D Biosensors

项目摘要

A long standing challenge in human engineering has been the identification, capture and analysis of single cells with high sensitivity and selectivity. This challenge is motivated by the need to understand progressive or dynamical events in tissues that might otherwise be averaged away in a population wide analysis. The PI proposes to utilize origami inspired folding designs to create 3D patterned autonomous biosensors that can be used to actively capture and analyze single cells in the same microstructure. Such devices which do not exist at the present time would lead to significantly improved biosensing protocols for lab-on-a-chip or array-based diagnostic devices, enable ultra-high resolution tissue sampling and provide new tools for scientific discovery. The proposal seeks to pattern transparent biocompatible thin films in 2D using high resolution lithographic approaches and engineer strain within these patterned films so that they fold up around single cells. We propose to develop such self-folding devices with biomolecular patterns and show that we are able to simultaneously perform a single cell capture and assay it using optical methods within a single self-folded structure in a parallel and high throughput manner. In addition to arrays on substrates, our methods could also be adapted to create free floating self-folding grippers for single cell excision and analysis from tissue samples. The approach provides a new paradigm for 3D single cell biosensors and we will integrate our research with educational and outreach efforts directed at K-12, undergraduate and graduate students.

人类工程学中的一个长期挑战是以高灵敏度和选择性识别、捕获和分析单个细胞。这一挑战的动机是需要了解组织中的进行性或动力学事件，否则这些事件可能会在全人群分析中平均消失。PI建议利用折纸灵感的折叠设计来创建3D图案化自主生物传感器，这种传感器可以用来主动捕获和分析相同微观结构中的单个细胞。这种目前尚不存在的设备将大大改进芯片上实验室或基于阵列的诊断设备的生物传感协议，使超高分辨率组织采样成为可能，并为科学发现提供新的工具。该提案寻求使用高分辨率光刻方法在2D中形成透明的生物兼容薄膜，并在这些图案化的薄膜中设计应变，以便它们在单个细胞周围折叠。我们建议开发这种具有生物分子图案的自折叠设备，并表明我们能够同时执行单个细胞捕获，并以并行和高通量的方式在单个自折叠结构中使用光学方法对其进行分析。除了衬底上的阵列，我们的方法还可以用于制造自由浮动的自折叠夹持器，用于从组织样本中进行单细胞切除和分析。该方法为3D单细胞生物传感器提供了一个新的范例，我们将把我们的研究与针对K-12、本科生和研究生的教育和推广工作结合起来。