EAPSI: Portable Smartphone-Based Detection of Vaspin Protein in Human Blood Serum

EAPSI：基于便携式智能手机的人血清中 Vaspin 蛋白的检测

• 批准号: 1714046
• 负责人: Tiffany-Heather Ulep
• 金额: $ 0.54万
• 依托单位: Ulep Tiffany-HeatherT
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1714046&HistoricalAwards=false
• 关键词: EAPSI; Portable; Smartphone; Based; Detection

Vaspin, a protein secreted from fat tissue, has been shown in numerous studies to be closely related to type 2 diabetes, insulin sensitivity, obesity, and glucose metabolism. Aptamers, highly stable and specific molecular probes, can be linked onto gold nanoparticles for its use in biosensor applications. This project will explore the use of this vaspin targeting gold nanoparticle in a paper microfluidic assay as a cheaper, disposable, and portable colorimetric indicator. A smartphone-integrated capability will be designed, developed, and used for image analysis to detect vaspin concentration in human blood serum. The aim of this project is to fabricate a portable, low cost, point of care (POC) system that can be used for diagnostics and monitoring for insulin-related metabolism in areas of low resources and accessibilities. This research will be conducted in collaboration with Dr. Man Bock Gu, a noted expert on aptamer production, characterization, and analysis, at Korea University. In numerous studies Vaspin, an adipocytokine secreted from adipose tissue, has been closely related to type 2 diabetes, insulin sensitivity, obesity, and glucose metabolism. Aptamers, oligonucleotide sequences that can specifically bind to certain molecules of interest, have been a topic of interest in biosensors for its biocompatibility, low cost, and chemical stability in comparison to antibodies. A newly-developed cognate duo aptamer (V1 and V49) targets vaspin at two different binding sites, further increasing specificity and affinity. Aptamer-gold nanoparticle (aptamer-AuNP) conjugations were used as biosensors to detect and measure vaspin concentrations in both buffer solutions and human blood serum dilutions via sandwich-type surface plasmon resonance (SPR) gold chips. Using this vaspin targeting aptamer-AuNP conjugate, a colorimetric paper microfluidic assay can be developed with use of smart-phone-integrated capabilities and analysis. The aim of this project is to fabricate a portable, low cost, point of care (POC), diagnostic system that will be beneficial to areas of low resources and accessibility to healthcare. This award, under the East Asian and Pacific Summer Institutes program, supports summer research by a U.S. graduate student and is jointly funded by NSF and the National Research Foundation of Korea.

Vaspin是一种由脂肪组织分泌的蛋白质，许多研究表明它与2型糖尿病、胰岛素敏感性、肥胖和葡萄糖代谢密切相关。核酸适体是一种高度稳定和特异的分子探针，可以连接到金纳米颗粒上用于生物传感器的应用。该项目将探索在纸微流控分析中使用这种vaspin靶向金纳米颗粒作为更便宜，一次性和便携式比色指示剂。智能手机集成的能力将被设计，开发，并用于图像分析，以检测人血清中的vaspin浓度。该项目的目的是制造一种便携式，低成本，护理点（POC）系统，可用于诊断和监测胰岛素相关的代谢在资源和可及性低的地区。这项研究将与韩国大学适体生产、表征和分析方面的著名专家Man Bock Gu博士合作进行。在大量研究中，Vaspin是一种由脂肪组织分泌的脂肪细胞因子，与2型糖尿病、胰岛素敏感性、肥胖和葡萄糖代谢密切相关。适体，可以特异性地结合到某些感兴趣的分子的寡核苷酸序列，由于其生物相容性、低成本和与抗体相比的化学稳定性，已经成为生物传感器中感兴趣的主题。一种新开发的同源双适体（V1和V49）在两个不同的结合位点靶向vaspin，进一步提高了特异性和亲和力。使用适配体-金纳米颗粒（适配体-AuNP）缀合物作为生物传感器，通过三明治型表面等离子体共振（SPR）金芯片检测和测量缓冲液和人血清稀释液中的vaspin浓度。使用这种vaspin靶向适体-AuNP缀合物，可以利用智能手机集成的能力和分析开发比色纸微流体测定。该项目的目的是制造一种便携式，低成本，护理点（POC），诊断系统，这将有利于资源和医疗保健的可及性低的地区。 该奖项是东亚和太平洋夏季研究所计划下的一个奖项，支持美国研究生的夏季研究，由NSF和韩国国家研究基金会共同资助。