Autophagy-dependent and -independent mechanisms of RNA transport to the vacuole and their role in rRNA turnover and cellular homeostasis

RNA 转运至液泡的自噬依赖性和非依赖性机制及其在 rRNA 周转和细胞稳态中的作用

• 批准号: 1714996
• 负责人: Gustavo MacIntosh
• 金额: $ 90万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1714996&HistoricalAwards=false
• 关键词: Autophagy; dependent; independent; mechanisms; RNA

The pathways by which plant cells recycle their constituents are critical for growth and environmental stress tolerance, yet our knowledge of these pathways is very limited. This project will address these limitations by identifying the mechanisms by which cellular components are recycled once they are damaged or no longer needed. A better understanding of these pathways will allow the development of crops and other plants with an optimized balance between growth and adaptation to stress conditions such as nutrient deficiencies and drought. A number of graduate and undergraduate students will be trained in research during this project, and each year a high school teacher will be involved in the research through an ongoing Research Experiences for Teachers program, potentially impacting large numbers of high school students. Enhancements will be made to an educational computer learning tool on cell biology and it will be introduced into Freshman Biology laboratories. Ribosomes are essential and highly abundant components of cells, yet their degradation and recycling pathways are unclear. The goal of this project is to decipher these pathways by analyzing the transport and degradation of ribosomes and ribosomal components within plant vacuoles. A newly discovered pathway for transport of ribosomal RNA will be characterized, using a fluorescent RNA transport assay together with analysis of mutants that are defective in factors required for this pathway. The way in which ribosomes are recognized and selected for degradation, for example by accumulating damage, will be determined biochemically. Metabolome analysis by mass spectrometry will allow the effect of loss of ribosome degradation pathways on cell metabolism and homeostasis to be assessed.

植物细胞回收其成分的途径对于生长和环境胁迫耐受性至关重要，但我们对这些途径的了解非常有限。该项目将通过确定细胞成分一旦损坏或不再需要时回收的机制来解决这些限制。更好地了解这些途径将使作物和其他植物的发展在生长和适应营养缺乏和干旱等胁迫条件之间达到最佳平衡。一些研究生和本科生将在这个项目期间接受研究培训，每年一名高中教师将通过正在进行的教师研究经验计划参与研究，可能会影响大量的高中学生。将加强一个关于细胞生物学的教育计算机学习工具，并将其引入新生生物学实验室。核糖体是细胞的基本和高度丰富的组成部分，但其降解和回收途径尚不清楚。该项目的目标是通过分析植物液泡中核糖体和核糖体组分的运输和降解来破译这些途径。一个新发现的核糖体RNA的运输途径的特点是，使用荧光RNA运输试验连同分析突变体，这是缺陷的因素，这条途径所需的。核糖体被识别和选择降解的方式，例如通过累积损伤，将由生物化学决定。通过质谱法进行的代谢组分析将允许评估核糖体降解途径的丧失对细胞代谢和稳态的影响。

项目成果

Editorial: Sugars and Autophagy in Plants

• DOI: 10.3389/fpls.2019.01190
• 发表时间: 2019-09
• 期刊: Frontiers in Plant Science
• 影响因子: 5.6
• 作者: S. Signorelli;C. Masclaux-Daubresse;Y. Moriyasu;W. Van den Ende;D. Bassham

Arabidopsis PAP17 is a dual-localized purple acid phosphatase up-regulated during phosphate deprivation, senescence, and oxidative stress

• DOI: 10.1093/jxb/erab409
• 发表时间: 2021-09
• 期刊: Journal of Experimental Botany
• 影响因子: 6.9
• 作者: Bryden O'Gallagher;M. Ghahremani;Kyla A. Stigter;E. L. Walker;Michal Pyc;Ang-Yu Liu;G. C. Macintosh;R. Mullen;W. Plaxton

TOR mediates the autophagy response to altered nucleotide homeostasis in an RNase mutant

• DOI: 10.1093/jxb/eraa410
• 发表时间: 2020-12-31
• 期刊: JOURNAL OF EXPERIMENTAL BOTANY
• 影响因子: 6.9
• 作者: Kazibwe, Zakayo;Soto-Burgos, Junmarie;Bassham, Diane C.
• 通讯作者: Bassham, Diane C.

Ups and downs of lysosomal pH: conflicting roles of LAMP proteins?

溶酶体 pH 值的起伏：LAMP 蛋白的相互冲突的作用？

• DOI: 10.1080/15548627.2023.2274253
• 发表时间: 2023
• 期刊: Autophagy
• 影响因子: 13.3
• 作者: Handy, Jonathan;Macintosh, Gustavo C.;Jenny, Andreas
• 通讯作者: Jenny, Andreas

Lamp1 mediates lipid transport, but is dispensable for autophagy in Drosophila

• DOI: 10.1080/15548627.2022.2038999
• 发表时间: 2022-03-11
• 期刊: AUTOPHAGY
• 影响因子: 13.3
• 作者: Chaudhry, Norin;Sica, Margaux;Jenny, Andreas
• 通讯作者: Jenny, Andreas