MRI: Acquisition of a flow cytometer to promote microbe-focused research, training, and teaching at NC State University

北卡罗来纳州立大学(NCSU)被授予购买荧光激活细胞分选(FACS)的细胞仪。此次收购将支持NCSU生物技术(BIT)计划中新的(如单细胞技术)和改进的(如免疫学和高通量发现)教学模块，该计划的主要目标是在生物技术领域教育和培训本科生和研究生。BIT之外的课程也将得到支持；细胞仪将使本科生遗传学以及各种环境微生物学领域(如水生微生物学、生物海洋学、河口生物地球化学)有更多现代化的实验室/教学。累计，350名学生预计将通过这些课程获得流式细胞仪技术的经验。超过25名研究生和博士后学者将通过NCSU利用该系统的研究项目获得FACS的直接经验。为了提高北卡罗来纳州罗利地区未被充分代表的本科生对FACS(和一般的流式细胞术)技术的认识，FACS和流式细胞术研讨会将每年举办一次，为期4年。这些研讨会的参与者将在北卡罗来纳州自然科学博物馆发表演讲，向公众传达他们由此产生的科学素养和知识。该奖项主要致力于促进NCSU以及邻近大学的多样化科学研究计划。将支持的科学研究领域包括：水生浮游生物生态、有害藻华生态、污染物降解菌检测、极端微生物生态、共生、抗生素和酶的快速发现、恐龙细胞的遗传鉴定以及双壳类营养和微生物群。仅在NCSU，这些研究小组就代表了四所学院，并得到了当前研究奖项的广泛支持。FACS系统支持的所有研究结果将在声誉良好的同行评议出版物和科学会议上传播。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

An award is made to North Carolina State University (NCSU) to purchase a cytometer for fluorescence activated cell sorting (FACS). The acquisition will support new (e.g., Single-cell Technologies) and revamped (e.g., Immunology and High-Throughput Discovery) teaching modules within NCSU's Biotechnology (BIT) program, whose primary goal is to educate and train undergraduate and graduate students in areas of biotechnology. Courses outside BIT will also be supported; the cytometer will enable more modern labs/instruction in undergraduate Genetics, as well as in various environmental microbiology areas (e.g., Aquatic Microbiology, Biological Oceanography, Estuary Biogeochemistry). Cumulatively, 350 students are anticipated to gain experience with flow cytometry technology through these courses. More than 25 graduate students and postdoctoral scholars will gain direct experience with FACS through NCSU research projects utilizing the system. To increase awareness of FACS (and flow cytometry in general) technology among underrepresented undergraduates in the Raleigh, NC, area, FACS & Flow Cytometry workshops will be conducted annually for 4 years. The participants in these workshops will make presentations at the NC Museum of Natural Science to convey their resulting scientific literacy and knowledge to the general public.The award is heavily devoted to promoting diverse scientific research programs at NCSU, as well as at neighboring universities. Areas of scientific research that will be supported include: aquatic plankton ecology, harmful algal bloom ecology, detection of pollutant degrading bacteria, extremophile microbial ecology, symbiosis, rapid antibiotic and enzyme discovery, genetic identification of dinosaur cells, and bivalve nutrition and microbiomes. At NCSU alone, these research groups represent four colleges and extensive support from current research awards. All results from the research enabled by the FACS system will be disseminated in reputable peer-reviewed publications and at scientific meetings.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.