MRI: ACQUISITION OF A PULSED ELECTRON SPIN RESONANCE SPECTROMETER TO AID BIOPHYSICAL AND CHEMICAL RESEARCH, EDUCATION AND OUTREACH

MRI：购买脉冲电子自旋共振波谱仪以帮助生物物理和化学研究、教育和推广

• 批准号: 1725678
• 负责人: Sunil Saxena
• 金额: $ 61.6万
• 依托单位: University of Pittsburgh
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1725678&HistoricalAwards=false
• 关键词: MRI; ACQUISITION; PULSED; ELECTRON; SPIN

This award is supported by the Major Research Instrumentation (MRI) and the Chemistry Research Instrumentation and Facilities (CRIF) Programs. Professor Sunil Saxena from University of Pittsburgh and colleagues are acquiring an X-/Q-band pulsed electron paramagnetic resonance (EPR) spectrometer. This instrument allows research in a variety of fields such as those that provide insight on how biologically relevant species with unpaired electrons behave. The instrument also allows researchers to conduct advanced environmental studies. In general, an EPR spectrometer yields detailed information on the geometric and electronic structure of molecular and solid state materials. It is used to obtain information about the lifetimes of unpaired electrons (free radicals) - a short-lived, highly reactive species - involved in valuable chemical transformations. A number of graduate and undergraduate students use the instrument. This spectrometer expands access to pulsed EPR instrumentation to undergraduates at a 4-year college in Pennsylvania (Westminster) College) and a predominantly African American institution (Fisk University). The spectrometer furthers the development of an EPR Center of Excellence that is regional in nature, thus having a broad impact in research infrastructure for the entire tri-state area. Current users include groups at University of Pittsburgh, Carnegie Mellon University, Ohio State University, Fisk University and Westminster College. The results of the research help modernize the high-school curriculum by rapid dissemination of research ideas to high-school teachers via workshops. The spectrometer is used in classes to expose students to modern ideas in biophysics. This EPR spectrometer enhances research and education at all levels. It allows sensitive delineation of backbone versus spin label motions in proteins and macromolecules ,and enables high resolution measurements to determine constraints on macromolecular structure and conformational dynamics. The spectrometer also allows discrimination of metal-protein interactions and enables the determination of the identity of transient and stable radicals.

该奖项由主要研究仪器 (MRI) 和化学研究仪器和设施 (CRIF) 项目支持。匹兹堡大学的 Sunil Saxena 教授及其同事正在购买一台 X/Q 波段脉冲电子顺磁共振 (EPR) 波谱仪。该仪器允许在各个领域进行研究，例如那些提供有关具有不成对电子的生物学相关物种如何行为的见解的领域。 该仪器还允许研究人员进行高级环境研究。一般来说，EPR 光谱仪可提供分子和固态材料的几何和电子结构的详细信息。它用于获取有关不成对电子（自由基）寿命的信息 - 一种短命的、高活性物质 - 参与有价值的化学转化。许多研究生和本科生使用该仪器。该光谱仪扩大了宾夕法尼亚州一所四年制大学（威斯敏斯特学院）和一所以非裔美国人为主的机构（菲斯克大学）的本科生使用脉冲 EPR 仪器的机会。该光谱仪进一步促进了区域性 EPR 卓越中心的发展，从而对整个三州地区的研究基础设施产生了广泛的影响。目前的用户包括匹兹堡大学、卡内基梅隆大学、俄亥俄州立大学、菲斯克大学和威斯敏斯特学院的团体。研究结果通过研讨会向高中教师快速传播研究思想，有助于实现高中课程的现代化。光谱仪在课堂上使用，让学生了解生物物理学的现代思想。这款 EPR 光谱仪可增强各个层面的研究和教育。它可以灵敏地描绘蛋白质和大分子中的主链与自旋标签运动，并实现高分辨率测量以确定对大分子结构和构象动力学的限制。该光谱仪还可以区分金属-蛋白质相互作用，并能够确定瞬态和稳定自由基的身份。

项目成果

Double Histidine Based EPR Measurements at Physiological Temperatures Permit Site‐Specific Elucidation of Hidden Dynamics in Enzymes

生理温度下基于双组氨酸的 EPR 测量允许位点特定阐明酶中隐藏的动力学

• DOI: 10.1002/ange.202009982
• 发表时间: 2020
• 期刊: Angewandte Chemie
• 作者: Singewald, Kevin;Bogetti, Xiaowei;Sinha, Kaustubh;Rule, Gordon S.;Saxena, Sunil
• 通讯作者: Saxena, Sunil

Cu(II) EPR Reveals Two Distinct Binding Sites and Oligomerization of Innate Immune Protein Calgranulin C

• DOI: 10.1007/s00723-018-1053-7
• 发表时间: 2018-11-01
• 期刊: APPLIED MAGNETIC RESONANCE
• 影响因子: 1
• 作者: Ghosh, Shreya;Garcia, Velia;Saxena, Sunil
• 通讯作者: Saxena, Sunil

Increasing nitroxide lifetime in cells to enable in-cell protein structure and dynamics measurements by electron spin resonance spectroscopy

• DOI: 10.1016/j.jmr.2018.12.005
• 发表时间: 2019-02-01
• 期刊: JOURNAL OF MAGNETIC RESONANCE
• 影响因子: 2.2
• 作者: Singewald, Kevin;Lawless, Matthew J.;Saxena, Sunil
• 通讯作者: Saxena, Sunil

Cu2+-based distance measurements by pulsed EPR provide distance constraints for DNA backbone conformations in solution

• DOI: 10.1093/nar/gkaa133
• 发表时间: 2020-05-21
• 期刊: NUCLEIC ACIDS RESEARCH
• 影响因子: 14.9
• 作者: Ghosh, Shreya;Lawless, Matthew J.;Saxena, Sunil
• 通讯作者: Saxena, Sunil

Effects of MnO2 of different structures on activation of peroxymonosulfate for bisphenol A degradation under acidic conditions

• DOI: 10.1016/j.cej.2019.03.238
• 发表时间: 2019-08-15
• 期刊: CHEMICAL ENGINEERING JOURNAL
• 影响因子: 15.1
• 作者: Huang, Jianzhi;Dai, Yifan;Zhang, Huichun
• 通讯作者: Zhang, Huichun